用于治疗糖尿病伤口的含桔梗提取物的电纺明胶伤口敷料:体外和体内研究。

IF 3.1 4区 医学 Q2 BIOPHYSICS Journal of Applied Biomaterials & Functional Materials Pub Date : 2024-01-01 DOI:10.1177/22808000241245298
Jing Zhang, Shen Shen, Shijie Zhu, Fan Jia, Jin Li, Yu Sun
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引用次数: 0

摘要

在目前的研究中,本草纲目提取物被载入电纺明胶支架,用于糖尿病伤口愈合。通过机械测试、细胞培养试验、电子显微镜、细胞迁移试验和抗菌试验对支架进行了体外表征。在大鼠糖尿病伤口模型中进行了体内伤口愈合研究。体外研究显示,我们开发的敷料具有纤维结构和抗炎特性。此外,敷料还能防止细菌渗透。体内研究表明,与其他组别相比,负载了蛇床子提取物的支架在伤口缩小、胶原沉积和上皮厚度方面都有显著提高。基因表达研究表明,所生产的伤口敷料能明显提高糖尿病伤口中血管内皮生长因子和 IGF 基因的表达。
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Cnicus benedictus extract-loaded electrospun gelatin wound dressing for treating diabetic wounds: An in vitro and in vivo study.

In the current study, Cnicus benedictus extract was loaded into electrospun gelatin scaffolds for diabetic wound healing applications. Scaffolds were characterized in vitro by mechanical testing, cell culture assays, electron microscopy, cell migration assay, and antibacterial assay. In vivo wound healing study was performed in a rat model of diabetic wound. In vitro studies revealed fibrous architecture of our developed dressings and their anti-inflammatory properties. In addition, Cnicus benedictus extract-loaded wound dressings prevented bacterial penetration. In vivo study showed that wound size reduction, collagen deposition, and epithelial thickness were significantly greater in Cnicus benedictus extract-loaded scaffolds than other groups. Gene expression studies showed that the produced wound dressings significantly upregulated VEGF and IGF genes expression in diabetic wounds.

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来源期刊
Journal of Applied Biomaterials & Functional Materials
Journal of Applied Biomaterials & Functional Materials BIOPHYSICS-ENGINEERING, BIOMEDICAL
CiteScore
4.40
自引率
4.00%
发文量
36
审稿时长
>12 weeks
期刊介绍: The Journal of Applied Biomaterials & Functional Materials (JABFM) is an open access, peer-reviewed, international journal considering the publication of original contributions, reviews and editorials dealing with clinical and laboratory investigations in the fast growing field of biomaterial sciences and functional materials. The areas covered by the journal will include: • Biomaterials / Materials for biomedical applications • Functional materials • Hybrid and composite materials • Soft materials • Hydrogels • Nanomaterials • Gene delivery • Nonodevices • Metamaterials • Active coatings • Surface functionalization • Tissue engineering • Cell delivery/cell encapsulation systems • 3D printing materials • Material characterization • Biomechanics
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