Ryan A. Dunn , Hui-Ying Luk , Casey R. Appell , Nigel C. Jiwan , Marcos S. Keefe , Jan-Joseph S. Rolloque , Yasuki Sekiguchi
{"title":"在高温下进行肌肉损伤性偏心运动，可在今后暴露于高温之前和之后立即降低细胞应力。","authors":"Ryan A. Dunn , Hui-Ying Luk , Casey R. Appell , Nigel C. Jiwan , Marcos S. Keefe , Jan-Joseph S. Rolloque , Yasuki Sekiguchi","doi":"10.1016/j.cstres.2024.05.001","DOIUrl":null,"url":null,"abstract":"<div><p>Muscle-damaging exercise (e.g., downhill running [DHR]) or heat exposure bouts potentially reduce physiological and/or cellular stress during future exertional heat exposure; however, the true extent of their combined preconditioning effects is unknown. Therefore, this study investigated the effect of muscle-damaging exercise in the heat on reducing physiological and cellular stress during future exertional heat exposure. Ten healthy males (mean ± Standard Definition; age, 23 ± 3 years; body mass, 78.7 ± 11.5 kg; height, 176.9 ± 4.7 cm) completed this study. Participants were randomly assigned into two preconditioning groups: (a) DHR in the heat (ambient temperature [T<sub>amb</sub>], 35 °C; relative humidity [RH], 40%) and (b) DHR in thermoneutral (T<sub>amb</sub>, 20 °C; RH, 20%). Seven days following DHR, participants performed a 45-min flat run in the heat (Flat<sub>HEAT</sub> [T<sub>amb</sub>, 35 °C; RH, 40%]). During exercise, heart rate and rectal temperature (T<sub>rec</sub>) were recorded at baseline and every 5-min. Peripheral blood mononuclear cells were isolated to assess heat shock protein 72 (Hsp72) concentration between conditions at baseline, immediately post-DHR, and immediately pre-Flat<sub>HEAT</sub> and post-Flat<sub>HEAT</sub>. Mean T<sub>rec</sub> during Flat<sub>HEAT</sub> between hot (38.23 ± 0.38 °C) and thermoneutral DHR (38.26 ± 0.38 °C) was not significantly different (<em>P</em> = 0.68), with no mean heart rate differences during Flat<sub>HEAT</sub> between hot (172 ± 15 beats min<sup>−1</sup>) and thermoneutral conditions (174 ± 8 beats min<sup>−1</sup>; <em>P</em> = 0.58). Hsp72 concentration change from baseline to immediately pre-Flat<sub>HEAT</sub> was significantly lower in hot (−51.4%) compared to thermoneutral (+24.2%; <em>P</em> = 0.025) DHR, with Hsp72 change from baseline to immediately post-Flat<sub>HEAT</sub> also lower in hot (−52.6%) compared to thermoneutral conditions (+26.3%; <em>P</em> = 0.047). A bout of muscle-damaging exercise in the heat reduces cellular stress levels prior to and immediately following future exertional heat exposure.</p></div>","PeriodicalId":9684,"journal":{"name":"Cell Stress & Chaperones","volume":"29 3","pages":"Pages 472-482"},"PeriodicalIF":3.3000,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1355814524000737/pdfft?md5=7527cb8830b42a0ea4d640a6302a437a&pid=1-s2.0-S1355814524000737-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Eccentric muscle-damaging exercise in the heat lowers cellular stress prior to and immediately following future exertional heat exposure\",\"authors\":\"Ryan A. Dunn , Hui-Ying Luk , Casey R. Appell , Nigel C. Jiwan , Marcos S. Keefe , Jan-Joseph S. Rolloque , Yasuki Sekiguchi\",\"doi\":\"10.1016/j.cstres.2024.05.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Muscle-damaging exercise (e.g., downhill running [DHR]) or heat exposure bouts potentially reduce physiological and/or cellular stress during future exertional heat exposure; however, the true extent of their combined preconditioning effects is unknown. Therefore, this study investigated the effect of muscle-damaging exercise in the heat on reducing physiological and cellular stress during future exertional heat exposure. Ten healthy males (mean ± Standard Definition; age, 23 ± 3 years; body mass, 78.7 ± 11.5 kg; height, 176.9 ± 4.7 cm) completed this study. Participants were randomly assigned into two preconditioning groups: (a) DHR in the heat (ambient temperature [T<sub>amb</sub>], 35 °C; relative humidity [RH], 40%) and (b) DHR in thermoneutral (T<sub>amb</sub>, 20 °C; RH, 20%). Seven days following DHR, participants performed a 45-min flat run in the heat (Flat<sub>HEAT</sub> [T<sub>amb</sub>, 35 °C; RH, 40%]). During exercise, heart rate and rectal temperature (T<sub>rec</sub>) were recorded at baseline and every 5-min. Peripheral blood mononuclear cells were isolated to assess heat shock protein 72 (Hsp72) concentration between conditions at baseline, immediately post-DHR, and immediately pre-Flat<sub>HEAT</sub> and post-Flat<sub>HEAT</sub>. Mean T<sub>rec</sub> during Flat<sub>HEAT</sub> between hot (38.23 ± 0.38 °C) and thermoneutral DHR (38.26 ± 0.38 °C) was not significantly different (<em>P</em> = 0.68), with no mean heart rate differences during Flat<sub>HEAT</sub> between hot (172 ± 15 beats min<sup>−1</sup>) and thermoneutral conditions (174 ± 8 beats min<sup>−1</sup>; <em>P</em> = 0.58). Hsp72 concentration change from baseline to immediately pre-Flat<sub>HEAT</sub> was significantly lower in hot (−51.4%) compared to thermoneutral (+24.2%; <em>P</em> = 0.025) DHR, with Hsp72 change from baseline to immediately post-Flat<sub>HEAT</sub> also lower in hot (−52.6%) compared to thermoneutral conditions (+26.3%; <em>P</em> = 0.047). A bout of muscle-damaging exercise in the heat reduces cellular stress levels prior to and immediately following future exertional heat exposure.</p></div>\",\"PeriodicalId\":9684,\"journal\":{\"name\":\"Cell Stress & Chaperones\",\"volume\":\"29 3\",\"pages\":\"Pages 472-482\"},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2024-05-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S1355814524000737/pdfft?md5=7527cb8830b42a0ea4d640a6302a437a&pid=1-s2.0-S1355814524000737-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Cell Stress & Chaperones\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1355814524000737\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CELL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cell Stress & Chaperones","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1355814524000737","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
Eccentric muscle-damaging exercise in the heat lowers cellular stress prior to and immediately following future exertional heat exposure
Muscle-damaging exercise (e.g., downhill running [DHR]) or heat exposure bouts potentially reduce physiological and/or cellular stress during future exertional heat exposure; however, the true extent of their combined preconditioning effects is unknown. Therefore, this study investigated the effect of muscle-damaging exercise in the heat on reducing physiological and cellular stress during future exertional heat exposure. Ten healthy males (mean ± Standard Definition; age, 23 ± 3 years; body mass, 78.7 ± 11.5 kg; height, 176.9 ± 4.7 cm) completed this study. Participants were randomly assigned into two preconditioning groups: (a) DHR in the heat (ambient temperature [Tamb], 35 °C; relative humidity [RH], 40%) and (b) DHR in thermoneutral (Tamb, 20 °C; RH, 20%). Seven days following DHR, participants performed a 45-min flat run in the heat (FlatHEAT [Tamb, 35 °C; RH, 40%]). During exercise, heart rate and rectal temperature (Trec) were recorded at baseline and every 5-min. Peripheral blood mononuclear cells were isolated to assess heat shock protein 72 (Hsp72) concentration between conditions at baseline, immediately post-DHR, and immediately pre-FlatHEAT and post-FlatHEAT. Mean Trec during FlatHEAT between hot (38.23 ± 0.38 °C) and thermoneutral DHR (38.26 ± 0.38 °C) was not significantly different (P = 0.68), with no mean heart rate differences during FlatHEAT between hot (172 ± 15 beats min−1) and thermoneutral conditions (174 ± 8 beats min−1; P = 0.58). Hsp72 concentration change from baseline to immediately pre-FlatHEAT was significantly lower in hot (−51.4%) compared to thermoneutral (+24.2%; P = 0.025) DHR, with Hsp72 change from baseline to immediately post-FlatHEAT also lower in hot (−52.6%) compared to thermoneutral conditions (+26.3%; P = 0.047). A bout of muscle-damaging exercise in the heat reduces cellular stress levels prior to and immediately following future exertional heat exposure.
期刊介绍：
Cell Stress and Chaperones is an integrative journal that bridges the gap between laboratory model systems and natural populations. The journal captures the eclectic spirit of the cellular stress response field in a single, concentrated source of current information. Major emphasis is placed on the effects of climate change on individual species in the natural environment and their capacity to adapt. This emphasis expands our focus on stress biology and medicine by linking climate change effects to research on cellular stress responses of animals, micro-organisms and plants.
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