酸挑战会加剧接受放射治疗的恒牙中基质金属蛋白酶的活化。

IF 1.5 4区 医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Brazilian oral research Pub Date : 2024-05-13 eCollection Date: 2024-01-01 DOI:10.1590/1807-3107bor-2024.vol38.0034
Alexandra Mussolino de Queiroz, Claudia María Carpio Bonilla, Taíssa Cássia de Souza Furtado, Regina Guenka Palma-Dibb, Harley Francisco de Oliveira, Maya Fernanda Manfrin Arnez, Fabrício Kitazono de Carvalho, Francisco Wanderley Garcia Paula-Silva
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引用次数: 0

摘要

本研究旨在探讨酸挑战对接受放射治疗的原牙和恒牙牙釉质交界处基质金属蛋白酶(MMPs)活化的影响。为此,研究人员使用了178颗从磨牙中提取的牙片,并将其随机分为2组(基牙和恒牙)/4个实验分组(辐照组和非辐照组、脱矿组和非脱矿组)。这些牙片连续 5 天接受剂量分数为 2 Gy 的辐射,直到总剂量达到 60 Gy,共 30 个周期,持续 6 周。为了确定牙本质釉质交界处(DEJ)上 MMPs 的活性,对 0.6 毫米的牙片进行了原位酶谱分析。为了评估 MMP 的活性是否会受到酸性环境的影响,将牙片置于脱矿物质溶液(pH 值为 4.8)中。结果发现,辐照激活了 DEJ 中的 MMPs,与基牙相比,这些影响在恒牙中更为明显。在研究酸挑战对 MMPs 活性的影响时,发现脱矿不会增加未受辐照牙齿中 MMPs 的活性,但会增加受辐照牙齿中 MMPs 的活性。总之,研究发现酸挑战会加剧辐照后恒牙DEJ中MMPs的活化,但不会加剧基牙中MMPs的活化。
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Acid challenge exacerbates activation of matrix metalloproteinases in permanent teeth undergoing radiotherapy.

The aim of this study was to investigate the effect of acid challenge on the activation of matrix metalloproteinases (MMPs) in the Dentinoenamel junction of primary and permanent teeth submitted to radiotherapy. For this purpose, a total of 178 dental fragments obtained from molars were used, and randomly divided into 2 groups (primary and permanent teeth) / 4 experimental subgroups (irradiated and non-irradiated, demineralized and non-demineralized). The fragments were exposed to radiation, with a dose fraction of 2 Gy, for 5 consecutive days, until a total dose of 60 Gy was reached, with a total of 30 cycles, for 6 weeks. To determine the activity of MMPs on the dentinoenamel junction (DEJ), in situ zymography assays on 0.6mm dental fragments were performed. To assess whether MMP activity would be impacted by an acidic environment, the fragments were placed in a demineralizing solution (pH of 4.8). The finding was that irradiation activated MMPs in DEJ and these effects were more evident in permanent when compared with primary teeth. When the effect of an acid challenge on MMPs activity was investigated, demineralization was observed not to increase MMPs activity in non-irradiated teeth, but it did increase MMPs activity in irradiated teeth. In conclusion, an acid challenge was found to exacerbate activation of MMPs in DEJ of permanent teeth submitted to irradiation, but not in primary teeth.

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来源期刊
CiteScore
3.70
自引率
4.00%
发文量
107
审稿时长
12 weeks
期刊最新文献
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