酸挑战会加剧接受放射治疗的恒牙中基质金属蛋白酶的活化。

IF 1.5 4区 医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Brazilian oral research Pub Date : 2024-05-13 eCollection Date: 2024-01-01 DOI:10.1590/1807-3107bor-2024.vol38.0034
Alexandra Mussolino de Queiroz, Claudia María Carpio Bonilla, Taíssa Cássia de Souza Furtado, Regina Guenka Palma-Dibb, Harley Francisco de Oliveira, Maya Fernanda Manfrin Arnez, Fabrício Kitazono de Carvalho, Francisco Wanderley Garcia Paula-Silva
{"title":"酸挑战会加剧接受放射治疗的恒牙中基质金属蛋白酶的活化。","authors":"Alexandra Mussolino de Queiroz, Claudia María Carpio Bonilla, Taíssa Cássia de Souza Furtado, Regina Guenka Palma-Dibb, Harley Francisco de Oliveira, Maya Fernanda Manfrin Arnez, Fabrício Kitazono de Carvalho, Francisco Wanderley Garcia Paula-Silva","doi":"10.1590/1807-3107bor-2024.vol38.0034","DOIUrl":null,"url":null,"abstract":"<p><p>The aim of this study was to investigate the effect of acid challenge on the activation of matrix metalloproteinases (MMPs) in the Dentinoenamel junction of primary and permanent teeth submitted to radiotherapy. For this purpose, a total of 178 dental fragments obtained from molars were used, and randomly divided into 2 groups (primary and permanent teeth) / 4 experimental subgroups (irradiated and non-irradiated, demineralized and non-demineralized). The fragments were exposed to radiation, with a dose fraction of 2 Gy, for 5 consecutive days, until a total dose of 60 Gy was reached, with a total of 30 cycles, for 6 weeks. To determine the activity of MMPs on the dentinoenamel junction (DEJ), in situ zymography assays on 0.6mm dental fragments were performed. To assess whether MMP activity would be impacted by an acidic environment, the fragments were placed in a demineralizing solution (pH of 4.8). The finding was that irradiation activated MMPs in DEJ and these effects were more evident in permanent when compared with primary teeth. When the effect of an acid challenge on MMPs activity was investigated, demineralization was observed not to increase MMPs activity in non-irradiated teeth, but it did increase MMPs activity in irradiated teeth. In conclusion, an acid challenge was found to exacerbate activation of MMPs in DEJ of permanent teeth submitted to irradiation, but not in primary teeth.</p>","PeriodicalId":9240,"journal":{"name":"Brazilian oral research","volume":"38 ","pages":"e034"},"PeriodicalIF":1.5000,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11376634/pdf/","citationCount":"0","resultStr":"{\"title\":\"Acid challenge exacerbates activation of matrix metalloproteinases in permanent teeth undergoing radiotherapy.\",\"authors\":\"Alexandra Mussolino de Queiroz, Claudia María Carpio Bonilla, Taíssa Cássia de Souza Furtado, Regina Guenka Palma-Dibb, Harley Francisco de Oliveira, Maya Fernanda Manfrin Arnez, Fabrício Kitazono de Carvalho, Francisco Wanderley Garcia Paula-Silva\",\"doi\":\"10.1590/1807-3107bor-2024.vol38.0034\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The aim of this study was to investigate the effect of acid challenge on the activation of matrix metalloproteinases (MMPs) in the Dentinoenamel junction of primary and permanent teeth submitted to radiotherapy. For this purpose, a total of 178 dental fragments obtained from molars were used, and randomly divided into 2 groups (primary and permanent teeth) / 4 experimental subgroups (irradiated and non-irradiated, demineralized and non-demineralized). The fragments were exposed to radiation, with a dose fraction of 2 Gy, for 5 consecutive days, until a total dose of 60 Gy was reached, with a total of 30 cycles, for 6 weeks. To determine the activity of MMPs on the dentinoenamel junction (DEJ), in situ zymography assays on 0.6mm dental fragments were performed. To assess whether MMP activity would be impacted by an acidic environment, the fragments were placed in a demineralizing solution (pH of 4.8). The finding was that irradiation activated MMPs in DEJ and these effects were more evident in permanent when compared with primary teeth. When the effect of an acid challenge on MMPs activity was investigated, demineralization was observed not to increase MMPs activity in non-irradiated teeth, but it did increase MMPs activity in irradiated teeth. In conclusion, an acid challenge was found to exacerbate activation of MMPs in DEJ of permanent teeth submitted to irradiation, but not in primary teeth.</p>\",\"PeriodicalId\":9240,\"journal\":{\"name\":\"Brazilian oral research\",\"volume\":\"38 \",\"pages\":\"e034\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2024-05-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11376634/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Brazilian oral research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1590/1807-3107bor-2024.vol38.0034\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Brazilian oral research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1590/1807-3107bor-2024.vol38.0034","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
引用次数: 0

摘要

本研究旨在探讨酸挑战对接受放射治疗的原牙和恒牙牙釉质交界处基质金属蛋白酶(MMPs)活化的影响。为此,研究人员使用了178颗从磨牙中提取的牙片,并将其随机分为2组(基牙和恒牙)/4个实验分组(辐照组和非辐照组、脱矿组和非脱矿组)。这些牙片连续 5 天接受剂量分数为 2 Gy 的辐射,直到总剂量达到 60 Gy,共 30 个周期,持续 6 周。为了确定牙本质釉质交界处(DEJ)上 MMPs 的活性,对 0.6 毫米的牙片进行了原位酶谱分析。为了评估 MMP 的活性是否会受到酸性环境的影响,将牙片置于脱矿物质溶液(pH 值为 4.8)中。结果发现,辐照激活了 DEJ 中的 MMPs,与基牙相比,这些影响在恒牙中更为明显。在研究酸挑战对 MMPs 活性的影响时,发现脱矿不会增加未受辐照牙齿中 MMPs 的活性,但会增加受辐照牙齿中 MMPs 的活性。总之,研究发现酸挑战会加剧辐照后恒牙DEJ中MMPs的活化,但不会加剧基牙中MMPs的活化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Acid challenge exacerbates activation of matrix metalloproteinases in permanent teeth undergoing radiotherapy.

The aim of this study was to investigate the effect of acid challenge on the activation of matrix metalloproteinases (MMPs) in the Dentinoenamel junction of primary and permanent teeth submitted to radiotherapy. For this purpose, a total of 178 dental fragments obtained from molars were used, and randomly divided into 2 groups (primary and permanent teeth) / 4 experimental subgroups (irradiated and non-irradiated, demineralized and non-demineralized). The fragments were exposed to radiation, with a dose fraction of 2 Gy, for 5 consecutive days, until a total dose of 60 Gy was reached, with a total of 30 cycles, for 6 weeks. To determine the activity of MMPs on the dentinoenamel junction (DEJ), in situ zymography assays on 0.6mm dental fragments were performed. To assess whether MMP activity would be impacted by an acidic environment, the fragments were placed in a demineralizing solution (pH of 4.8). The finding was that irradiation activated MMPs in DEJ and these effects were more evident in permanent when compared with primary teeth. When the effect of an acid challenge on MMPs activity was investigated, demineralization was observed not to increase MMPs activity in non-irradiated teeth, but it did increase MMPs activity in irradiated teeth. In conclusion, an acid challenge was found to exacerbate activation of MMPs in DEJ of permanent teeth submitted to irradiation, but not in primary teeth.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
3.70
自引率
4.00%
发文量
107
审稿时长
12 weeks
期刊最新文献
Assessment of Brazilian hospital and healthcare service infrastructure for cleft lip and palate patients. Comparison of the analgesic efficacy of spray and tablet flurbiprofen for pain after soft tissue surgery. Efficacy of xenogeneic collagen matrix in the treatment of gingival recessions: a controlled clinical trial. Facial morphology analysis of Caucasian Brazilian adult women using stereophotogrammetry. Impact of an educational intervention regarding tobacco counseling on dentists and dental students.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1