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引用次数: 0
摘要
丛枝菌根(AM)共生是 AM 真菌与大多数植物物种之间的营养合作关系,在全球无处不在,具有重要的生态和农业意义。研究 AM 共生的过程受到其高度时空动态性质的困扰。虽然已有显微镜方法可以探测这种植物-真菌相互作用的空间方面,但时间方面仍然更具挑战性,因为可靠的深组织延时成像要求共生双方在长时间内保持不受干扰。在这里,我们介绍 AMSlide:一种非侵入式、高分辨率、实时成像系统,是 AM 共生研究的最佳选择。我们展示了 AMSlide 在菌根共聚焦显微镜中的应用,从整个定植区到亚细胞结构,时间范围从几分钟到几周不等。此外,还概述了 AMSlide 在不同显微镜设置、成像技术以及植物和真菌物种方面的多功能性。希望 AMSlide 能在未来的研究中得到应用,以填补我们对 AM 共生以及更广泛的根部和根圈过程的认识上的时间空白。
The AMSlide for noninvasive time-lapse imaging of arbuscular mycorrhizal symbiosis.
Arbuscular mycorrhizal (AM) symbiosis, the nutritional partnership between AM fungi and most plant species, is globally ubiquitous and of great ecological and agricultural importance. Studying the processes of AM symbiosis is confounded by its highly spatiotemporally dynamic nature. While microscopy methods exist to probe the spatial side of this plant-fungal interaction, the temporal side remains more challenging, as reliable deep-tissue time-lapse imaging requires both symbiotic partners to remain undisturbed over prolonged time periods. Here, we introduce the AMSlide: a noninvasive, high-resolution, live-imaging system optimised for AM symbiosis research. We demonstrate the AMSlide's applications in confocal microscopy of mycorrhizal roots, from whole colonisation zones to subcellular structures, over timeframes from minutes to weeks. The AMSlide's versatility for different microscope set-ups, imaging techniques, and plant and fungal species is also outlined. It is hoped that the AMSlide will be applied in future research to fill in the temporal blanks in our understanding of AM symbiosis, as well as broader root and rhizosphere processes.
期刊介绍:
The Journal of Microscopy is the oldest journal dedicated to the science of microscopy and the only peer-reviewed publication of the Royal Microscopical Society. It publishes papers that report on the very latest developments in microscopy such as advances in microscopy techniques or novel areas of application. The Journal does not seek to publish routine applications of microscopy or specimen preparation even though the submission may otherwise have a high scientific merit.
The scope covers research in the physical and biological sciences and covers imaging methods using light, electrons, X-rays and other radiations as well as atomic force and near field techniques. Interdisciplinary research is welcome. Papers pertaining to microscopy are also welcomed on optical theory, spectroscopy, novel specimen preparation and manipulation methods and image recording, processing and analysis including dynamic analysis of living specimens.
Publication types include full papers, hot topic fast tracked communications and review articles. Authors considering submitting a review article should contact the editorial office first.