Mengjie Yang, Haisheng Zhang, Ethan Burcar, William Winchester, Erin Witherspoon, Zeinhom M. El-Bahy, Mohamed H. Helal, Hui Dang, Mohammed A. Amin, Baoshan Zhang, Yu Zhao, Xianpan Bu, Zhe Wang
{"title":"在真菌生长过程中，黄曲霉和金耳朵菌丝体相互抑制以防止竞争性病害的发生","authors":"Mengjie Yang, Haisheng Zhang, Ethan Burcar, William Winchester, Erin Witherspoon, Zeinhom M. El-Bahy, Mohamed H. Helal, Hui Dang, Mohammed A. Amin, Baoshan Zhang, Yu Zhao, Xianpan Bu, Zhe Wang","doi":"10.1007/s42114-024-00898-3","DOIUrl":null,"url":null,"abstract":"<div><p><i>Auricularia auricula</i> is one of the main edible fungi widely cultivated in China. <i>Aspergillus flavus</i> is the most common class of pathogenic bacteria fungi that produces the high toxicity of the aflatoxins, which is one of the fungal diseases of <i>A. auricula.</i> Morphological observation of <i>A. flavus</i> mycelium and <i>A. auricula</i> mycelium in plate confrontation, mutual inhibition of growth at different germination times, and the interaction of <i>A. flavus</i> liquid culture solution and <i>A. flavus</i> volatiles with <i>A. auricula</i> mycelium were used to investigate the mechanism of the interaction between <i>A. flavus</i> and <i>A. auricula</i> mycelium. Mycelium of <i>A. auricula</i> and <i>A. flavus</i> had a mutual inhibitory effect, but <i>A. auricula</i> mycelia had a stronger inhibitory effect on the growth of <i>A. flavus</i> mycelia. The results of the interaction between <i>A. flavus</i> volatiles and <i>A. auricula</i> volatiles were also the same and the inhibition of <i>A. flavus</i> by volatiles from <i>A. auricula</i> mycelium about 11%. After 240 h, the inhibition rate of <i>A. flavus</i> liquid culture solution on <i>A. auricula</i> mycelium reached up to about 20%. Some antimicrobial substances such as small peptides and organic acids produced in the metabolites of <i>A. flavus</i> liquid culture solution were the main reasons of the growth inhibition of <i>A. auricula</i> mycelium. The main inhibitory substances were 2-butanone, 2-butanone dimer, etc. Further study of AFT B<sub>1</sub> and AFT B<sub>2</sub> revealed that aflatoxins could migrate from the medium to the mycelia and the fruiting body of <i>A. auricula</i>, but the migration rate was basically lower than 10‱. The mycelia of <i>A. auricula</i> had a strong degradation of aflatoxins in the growth process. So, the mechanism of antifungal activity of these substances was studied to provide a theoretical basis for future chemical synthesis.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7220,"journal":{"name":"Advanced Composites and Hybrid Materials","volume":null,"pages":null},"PeriodicalIF":23.2000,"publicationDate":"2024-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Mutual inhibition of Aspergillus flavus and Auricularia auricula mycelium for the prevention of competing diseases during growth of fungi\",\"authors\":\"Mengjie Yang, Haisheng Zhang, Ethan Burcar, William Winchester, Erin Witherspoon, Zeinhom M. El-Bahy, Mohamed H. Helal, Hui Dang, Mohammed A. Amin, Baoshan Zhang, Yu Zhao, Xianpan Bu, Zhe Wang\",\"doi\":\"10.1007/s42114-024-00898-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><i>Auricularia auricula</i> is one of the main edible fungi widely cultivated in China. <i>Aspergillus flavus</i> is the most common class of pathogenic bacteria fungi that produces the high toxicity of the aflatoxins, which is one of the fungal diseases of <i>A. auricula.</i> Morphological observation of <i>A. flavus</i> mycelium and <i>A. auricula</i> mycelium in plate confrontation, mutual inhibition of growth at different germination times, and the interaction of <i>A. flavus</i> liquid culture solution and <i>A. flavus</i> volatiles with <i>A. auricula</i> mycelium were used to investigate the mechanism of the interaction between <i>A. flavus</i> and <i>A. auricula</i> mycelium. Mycelium of <i>A. auricula</i> and <i>A. flavus</i> had a mutual inhibitory effect, but <i>A. auricula</i> mycelia had a stronger inhibitory effect on the growth of <i>A. flavus</i> mycelia. The results of the interaction between <i>A. flavus</i> volatiles and <i>A. auricula</i> volatiles were also the same and the inhibition of <i>A. flavus</i> by volatiles from <i>A. auricula</i> mycelium about 11%. After 240 h, the inhibition rate of <i>A. flavus</i> liquid culture solution on <i>A. auricula</i> mycelium reached up to about 20%. Some antimicrobial substances such as small peptides and organic acids produced in the metabolites of <i>A. flavus</i> liquid culture solution were the main reasons of the growth inhibition of <i>A. auricula</i> mycelium. The main inhibitory substances were 2-butanone, 2-butanone dimer, etc. Further study of AFT B<sub>1</sub> and AFT B<sub>2</sub> revealed that aflatoxins could migrate from the medium to the mycelia and the fruiting body of <i>A. auricula</i>, but the migration rate was basically lower than 10‱. The mycelia of <i>A. auricula</i> had a strong degradation of aflatoxins in the growth process. 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Mutual inhibition of Aspergillus flavus and Auricularia auricula mycelium for the prevention of competing diseases during growth of fungi
Auricularia auricula is one of the main edible fungi widely cultivated in China. Aspergillus flavus is the most common class of pathogenic bacteria fungi that produces the high toxicity of the aflatoxins, which is one of the fungal diseases of A. auricula. Morphological observation of A. flavus mycelium and A. auricula mycelium in plate confrontation, mutual inhibition of growth at different germination times, and the interaction of A. flavus liquid culture solution and A. flavus volatiles with A. auricula mycelium were used to investigate the mechanism of the interaction between A. flavus and A. auricula mycelium. Mycelium of A. auricula and A. flavus had a mutual inhibitory effect, but A. auricula mycelia had a stronger inhibitory effect on the growth of A. flavus mycelia. The results of the interaction between A. flavus volatiles and A. auricula volatiles were also the same and the inhibition of A. flavus by volatiles from A. auricula mycelium about 11%. After 240 h, the inhibition rate of A. flavus liquid culture solution on A. auricula mycelium reached up to about 20%. Some antimicrobial substances such as small peptides and organic acids produced in the metabolites of A. flavus liquid culture solution were the main reasons of the growth inhibition of A. auricula mycelium. The main inhibitory substances were 2-butanone, 2-butanone dimer, etc. Further study of AFT B1 and AFT B2 revealed that aflatoxins could migrate from the medium to the mycelia and the fruiting body of A. auricula, but the migration rate was basically lower than 10‱. The mycelia of A. auricula had a strong degradation of aflatoxins in the growth process. So, the mechanism of antifungal activity of these substances was studied to provide a theoretical basis for future chemical synthesis.
期刊介绍：
Advanced Composites and Hybrid Materials is a leading international journal that promotes interdisciplinary collaboration among materials scientists, engineers, chemists, biologists, and physicists working on composites, including nanocomposites. Our aim is to facilitate rapid scientific communication in this field.
The journal publishes high-quality research on various aspects of composite materials, including materials design, surface and interface science/engineering, manufacturing, structure control, property design, device fabrication, and other applications. We also welcome simulation and modeling studies that are relevant to composites. Additionally, papers focusing on the relationship between fillers and the matrix are of particular interest.
Our scope includes polymer, metal, and ceramic matrices, with a special emphasis on reviews and meta-analyses related to materials selection. We cover a wide range of topics, including transport properties, strategies for controlling interfaces and composition distribution, bottom-up assembly of nanocomposites, highly porous and high-density composites, electronic structure design, materials synergisms, and thermoelectric materials.
Advanced Composites and Hybrid Materials follows a rigorous single-blind peer-review process to ensure the quality and integrity of the published work.
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