血清 IgG 免疫球蛋白水平与自然感染美洲野牛(Bison bison)中牛支原体 PCR 检测率降低有关。

IF 1.1 4区 农林科学 Q3 VETERINARY SCIENCES Journal of Wildlife Diseases Pub Date : 2024-07-01 DOI:10.7589/JWD-D-23-00151
Bryan S Kaplan, Jennifer L Malmberg, Kerry S Sondgeroth, Kaitlyn Sarlo Davila, Rohana P Dassanayake, Randy E Sacco, Eduardo Casas, Danielle E Buttke
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引用次数: 0

摘要

牛支原体是北美野牛(Bison bison)的一种重要病原体,与高发病率和高死亡率的呼吸道和生殖疾病流行有关。尽管这种疾病对野牛的健康有很大的负面影响,但人们对其发病动力学和宿主对感染的免疫反应却知之甚少。为了解决这些问题,我们建立了一个野牛小牛群,并在 12 个月的时间内对其进行了 5 次连续采样,每 2-3 个月采样一次。每次采样时都会采集鼻拭子样本,并通过 PCR 检测是否存在牛乳杆菌。此外,还收集血清样本,并使用商业和内部 ELISA 方法评估牛海绵状芽孢杆菌特异性抗体。总体而言,PCR 检测呈阳性的野牛有 19/41 头(46.3%),血清阳性的野牛有 31/41 头(75.6%)。在研究过程中,PCR 阳性鼻拭子的频率和 ELISA 分数都有所下降,但在最后一次 PCR 阳性检测后的至少 6 个月内,血清样本仍呈阳性。根据内部 ELISA 的结果,野牛被分为高应答组(7 头)、低应答组(5 头)和血清阴性组(7 头)。与低应答组和血清阴性组相比,高应答组的牛支原体特异性 IgG 抗体水平明显升高。其中 3/5 个采样期的差异具有统计学意义。高应答组的 IgG2 水平呈上升趋势。高总 IgG 反应与鼻拭子 PCR 检测阳性率下降相关。这些数据证明,强烈的体液反应是有益的,而且很可能参与了野牛牛线虫的清除。
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Serum IgG Immunoglobulin Levels are Associated with Reduced PCR Detection of Mycoplasma bovis in Naturally Infected American Bison (Bison bison).

Mycoplasma bovis (M. bovis) is an important pathogen of American bison (Bison bison), associated with high morbidity and mortality epizootics of respiratory and reproductive disease. Despite the significant negative impact on bison health, little is known about the kinetics of disease and the host immune response to infection. To address these questions, a cohort of bison calves was created and serially sampled 5 times, once every 2-3 mo, over a 12-mo period. At each sampling period nasal swab samples were collected and tested by PCR for the presence of M. bovis. Serum samples were also collected and assessed for M. bovis-specific antibodies using both a commercial and an in-house ELISA. Overall, 19/41 bison (46.3%) had positive PCR tests, and 31/41 (75.6%) were seropositive. Over the course of the study, the frequency of PCR-positive nasal swabs and the ELISA scores decreased, although serum samples remained positive for at least 6 mo following the final positive PCR test. Bison were grouped according to results from the in-house ELISA into high-responder (n=7), low-responder (n=5), and seronegative (n=7) groups. M. bovis-specific IgG antibody levels were significantly elevated in the high-responder group compared to the low-responder and seronegative groups. The differences were statistically significant for 3/5 sampling periods. A trend toward increased IgG2 levels was observed in the high-responder group. High total IgG responses correlated with a decline in positive PCR tests from nasal swabs. These data provide evidence that a strong humoral response is beneficial and is probably involved in the clearance of M. bovis from bison.

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来源期刊
Journal of Wildlife Diseases
Journal of Wildlife Diseases 农林科学-兽医学
CiteScore
2.70
自引率
0.00%
发文量
213
审稿时长
6-16 weeks
期刊介绍: The JWD publishes reports of wildlife disease investigations, research papers, brief research notes, case and epizootic reports, review articles, and book reviews. The JWD publishes the results of original research and observations dealing with all aspects of infectious, parasitic, toxic, nutritional, physiologic, developmental and neoplastic diseases, environmental contamination, and other factors impinging on the health and survival of free-living or occasionally captive populations of wild animals, including fish, amphibians, reptiles, birds, and mammals. Papers on zoonoses involving wildlife and on chemical immobilization of wild animals are also published. Manuscripts dealing with surveys and case reports may be published in the Journal provided that they contain significant new information or have significance for better understanding health and disease in wild populations. Authors are encouraged to address the wildlife management implications of their studies, where appropriate.
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