从巴基斯坦旁遮普省拉合尔市的羊肉中分离和鉴定产气荚膜梭菌及其毒素。

IF 0.5 4区 农林科学 Q4 VETERINARY SCIENCES Veterinaria italiana Pub Date : 2023-12-31 DOI:10.12834/VetIt.2926.20055.3
Suqaina Tul Abiha, Shahan Azeem, Aftab Ahmad Anjum, Muhammad Hassan Mushtaq
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引用次数: 0

摘要

本研究旨在检测巴基斯坦旁遮普省拉合尔市收集的羊肉样本中的产气荚膜杆菌并鉴定其毒素。研究人员从该市四个地区的当地肉店和零售店共采集了 40 份碎羊肉和非碎羊肉样本。对样品进行酶联免疫吸附试验(ELISA),以检测产气荚膜杆菌的α、β和ε毒素。同时对样本进行细菌分离处理。通过生化测试和针对产气荚膜杆菌的α、β和epsilon毒素基因的多重聚合酶链式反应,对分离出的细菌进行了确认。10%的样本(4/40)对产气荚膜杆菌α毒素呈阳性,17.5%的样本(7/40)对α毒素基因呈阳性。本研究表明,从本地肉店采集的样本受到产气荚膜杆菌及其毒素的污染。有趣的是,从肉类零售店采集的样本均未检出此类污染。总之,肉店的卫生条件不当可能导致羊肉受到产气荚膜杆菌及其毒素的污染。
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Isolation and identification of Clostridium perfringens and its toxins from mutton in Lahore City, Punjab, Pakistan.

The present study was aimed to detect C. perfringens and identify its toxins in mutton samples collected from Lahore City in the Punjab Province of Pakistan. A total of 40 samples of minced and non‑minced mutton were collected from local butcher and retail shops representing four areas of the city. The samples were subjected to ELISA for the detection of C. perfringens alpha, beta and epsilon toxins. The samples were simultaneously processed for bacterial isolation. The isolates were confirmed both by biochemical testing and a multiplex PCR targeting alpha, beta and epsilon toxin genes of C. perfringens. While 10% (4/40) of the samples were positive for C. perfringens alpha toxins, 17.5% (7/40) of the samples were positive for the alpha toxin gene. The present study indicated that the samples collected from the local butcher shops were contaminated with C. perfringens and its toxins. Interestingly, no such contamination was detected in any of the samples collected from retail meat shops. In conclusion, improper hygienic conditions at butcher shops could lead to the contamination of mutton with C. perfringens and its toxins.

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来源期刊
Veterinaria italiana
Veterinaria italiana VETERINARY SCIENCES-
CiteScore
1.50
自引率
0.00%
发文量
2
审稿时长
>12 weeks
期刊介绍: The journal was created as the Croce Azzurra in 1950. A quarterly peer-reviewed journal devoted to veterinary public health and other aspects of veterinary science and medicine, Veterinaria Italiana is published by the Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ (Istituto Zooprofilattico Sperimentale dell''Abruzzo e del Molise) in Teramo, Italy. The goal of the journal is to provide an international platform for veterinary public health information from Italy and other countries, particularly those in Eastern Europe and Africa, Asia and South America. Veterinarians and veterinary public health specialists are encouraged to share their knowledge and experience on this platform.
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