与纸浆和储存的玉米淀粉有关的微生物的分离和鉴定

O. J.O., O. J.I, Aphiar, A.E., Ukolobi O.
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引用次数: 0

摘要

这项研究分离并鉴定了与纸浆和储存的玉米淀粉有关的微生物。这样做是为了帮助描述与储存的玉米淀粉和纸浆有关的腐败微生物。这项研究是通过对纸浆样本和储存了 7 天的样本进行实验室分析实现的。结果显示,纸浆样品受到细菌污染。评估的细菌概况显示,在保存期间,细菌数量呈上升趋势,分离出的细菌包括假单胞菌、芽孢杆菌和乳酸杆菌,这些细菌在加热制备(纸浆)时并没有被完全消除。从纸浆样本中分离出的真菌表明,纸浆可能是潜在病原微生物的传播途径。不过,纸浆中没有分离出根瘤菌和曲霉菌等真菌,因为它们属于中嗜性真菌,无法在 60OC 以上的温度范围内存活。对样品的比较表明,纸浆制备是一种有效的消灭真菌的技术,但在彻底消灭细菌方面效果明显不佳。因此可以得出结论,烹饪纸浆并不能完全消除病原微生物,因此在食用纸浆时应谨慎。
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Isolation and Identification of Microorganisms Associated with Pap and Stored Corn Starch
This study isolated and identified microorganisms associated with pap and stored corn starch. This was done to help portray spoilage microorganisms associated with stored corn starch and pap. The study was achieved through laboratory analysis of pap sample and stored sample over a period of 7days. Result obtained shows that the pap samples are contaminated with bacteria. The profile of bacteria evaluated shows an increasing trend over the retention period with isolates of bacteria such as Pseudomonas sp., Bacillus sp and Lactobacillus sp. which are not entirely eliminated when prepared in heat (pap). The isolation of fungi from the pap samples revealed that it could serve as a means for the transmission of potentially pathogenic microorganisms. However, fungi isolates such Rhizopus sp. and Aspergillus sp. was not present in pap due to the fact that they are mesophilic fungi and cannot survive at temperature ranges above 60OC. Comparison of samples showed that pap preparation is an efficient technique for elimination of fungi but significantly ineffective in complete elimination of bacteria. It can therefore be concluded that cooking of pap does not entirely eliminate pathogenic microbes and hence caution should be taken in pap consumption.
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