从葫芦科植物唾液中生物合成的银纳米粒子对 HepG2 细胞的细胞毒性作用

Luma Jasim Witwit
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引用次数: 0

摘要

原发性肝细胞癌是一种毁灭性的肝癌。银纳米粒子(AgNPs)已被评估用于多种用途,包括作为抗癌剂进行测试。本研究旨在通过简单的 MTT 试验,评估从水蛭唾液中生物合成的 AgNPs 对 HepG2 细胞的细胞毒性。HepG2 细胞来自伊朗巴斯德研究所的细胞库。在这项研究中,以每孔 104 个细胞的密度培养 AgNP 处理过的 HepG2 细胞,并在每孔中加入 100 μL 浓度为 0.5 mg/mL 的 MTT,然后让处理过的细胞培养 4 小时。具有代表性的细胞图像显示,当 AgNP 浓度为 25 和 50 μg/mL 时,细胞形态发生了显著变化。48 小时后,纳米粒子的 IC50 值为 50 μg/mL。我们的研究表明,水蛭唾液提取物衍生的 AgNPs 对 HepG2 细胞具有细胞毒性。
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Cytotoxic effect of silver nanoparticles biosynthesized from Hirudo medicinalis saliva on HepG2 cells
Primary hepatocellular carcinoma is a devastating type of liver cancer. Silver nanoparticles (AgNPs) have been assessed for a variety of purposes, including being tested as an anticancer agent. The aim of this study was to assess the cytotoxicity of AgNPs that were biosynthesized from leech saliva on HepG2 cells, through the undertaking of a simple MTT assay. HepG2 cells were obtained from the cell bank of the Pasteur Institute of Iran. In this study, AgNP-treated HepG2 cells were cultured at a density of 104 cells per well, and 100 μL of MTT at a concentration of 0.5 mg/mL were added to each well; the treated cells were then let to incubate for 4 h. Subsequently, a plate reader device operating at a wavelength of 570 nm was used in order to determine the concentration of the chemical dissolved in isopropanol. Representative images of the cells show remarkable changes in their morphology at AgNP concentrations of 25 and 50 μg/mL. At 48 h, the nanoparticle’s IC50 value was 50 μg/mL. Our study shows that leech salivary extract-derived AgNPs are cytotoxic to HepG2 cells.
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