伊拉克库尔德斯坦地区埃尔比勒省已接种和未接种疫苗的 SARS-CoV-2 变体的分子流行病学研究

Dlshad Abdullah Hasan, S. Maulud, R. F. Rashid, Jivan Qasim, Rezhna Kheder, Article Info
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摘要

自 COVID-19 大流行以来,全球出现了多个新的 SARS-CoV-2 变异株 (VOC)。随着病毒的不断进化,预计还会出现更多变种。这就强调了对快速诊断方法的需求,以检测流行的变异株。变异株特异性实时反转录(rRT)-PCR 方法可用于替代基因组测序,而基因组测序在确定这些变异株方面既昂贵又费力,尤其是在资源有限的情况下。我们采用基于 RT-PCR 的诊断筛选方法评估了埃尔比勒省传播的各种 SARS-CoV-2 变异株的流行情况。我们使用 ViroQ® SC2 变异 rRT-PCR 对 144 份 SARS-CoV-2 阳性样本进行了已知 SARS-CoV2 变异的前瞻性检测。此外,还使用 25 份 SARS-CoV-2 阴性鼻腔样本对该技术进行了验证。在 144 份 SARS-CoV-2 阳性样本中,118 份(81.9%)为 B.1.617.2(Delta),5 份(3.5%)为 Epsilon B.1.427/B.1.429,1 份(0.7%)为 Eta B.1.525,2 份(1.4%)为 SARS-CoV-2 野生型,18 份(12.5%)为未定义的变异株,Delta 株是最常见的 SARS-CoV-2 株。我们的研究表明,变异株特异性 RT-PCR 是快速筛选 SARS-CoV-2 变异株的有效工具。
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Molecular Epidemiology of SARS-CoV-2 Variants in Vaccinated and Non-Vaccinated Patients of Erbil Province, Kurdistan Region-Iraq
Multiple new SARS-CoV-2 variants of concern (VOC) have emerged globally since the onset of the COVID-19 pandemic. With the virus continuing to evolve, more are expected. This emphasizes the need for rapid diagnostic methods for the detection of circulating lineages. Variants-specific real-time reverse transcription (rRT)-PCR method can be used as an alternative to genome sequencing, which is expensive and labored for identifying these variants, especially in settings with limited resources. We assessed the prevalence of various SARS-CoV-2 variants spreading in the Erbil province using a diagnostic screening RT-PCR-based method. A total of 144 SARS-CoV-2 positive samples were prospectively tested for known SARS-CoV2 variants using ViroQ® SC2 Variant rRT-PCR. Furthermore, the technique was validated using 25 SARS-CoV-2 negative nasal samples. Out of 144 SARS-CoV-2 positive samples, 118 (81.9%) were B.1.617.2 (Delta), 5 (3.5%) were Epsilon B.1.427/B.1.429, 1(0.7%) was Eta B.1.525, 2(1.4%) were SARS-CoV-2 Wild type, while 18 (12.5%) were undefined variant, and the delta strain was the most prevalent SARS-CoV-2 strain. Our study showed that variant-specific RT-PCR could be a useful tool for the rapid screening of SARS-CoV-2 variants.
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