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Evaluating IgG Levels, Vaccination Effects, and COVID-19 Infection Severity in Renal Transplant Recipients 评估肾移植受者的 IgG 水平、疫苗接种效果和 COVID-19 感染严重程度
Pub Date : 2024-05-03 DOI: 10.59786/bmtj.214
Zana Mohammed, Awny Jafar Ahmed, M. H. Mustafa, Domiana Shamoon Warda, Masood Ahmed Hameed, N. Hussein
Several studies have reported that different factors play an important role in the production of IgG after VOVID-19 vaccination. This study aimed at studying COVID-19 infection rates and severity in renal transplant recipients and vaccine responsiveness. comorbidity hemodialysis requirements, and immune suppression treatment regimens. In this cross-sectional study, IgG levels was measured in renal transplant recipients using automated benchtop immunoanalyzer Vidas at the Duhok Center for Kidney Disease and Transplantation. In this study group, only 30 individuals experienced the SARS-COVID-2 infection, with only three cases being severe. The most frequently reported symptoms of SARS-COVID-2 virus infection were fatigue (18/30) 60%, high temperature (17/30) 56%, headache (12/30) 40%, and poor endurance (11/30) 36.6%. The IgG titers were significantly different between the vaccinated 33.067 ± 2.5 versus unvaccinated individuals 23.916 ± 3.31with p value = 0.025.  On the other hand, demographic characteristics of the cohort including: comorbidities, hemodialysis, different age groups, gender, infection/no-infection differences had no statistically significant impact on the IgG titers.  Vaccination significantly increased the levels of protective IgG level in kidney transplant recipients. It is crucial to focus on increasing the acceptance of vaccination among kidney transplant recipients to prevent infections from other COVID-19 variants or pathogen outbreaks.
一些研究报告称,不同因素在接种 VOVID-19 疫苗后产生 IgG 的过程中起着重要作用。本研究旨在研究肾移植受者的 COVID-19 感染率和严重程度以及疫苗反应性、合并症、血液透析要求和免疫抑制治疗方案。在这项横断面研究中,杜霍克肾病和移植中心使用自动台式免疫分析仪 Vidas 对肾移植受者的 IgG 水平进行了测量。在该研究小组中,只有 30 人感染了 SARS-COVID-2,其中只有 3 例病情严重。最常报告的 SARS-COVID-2 病毒感染症状是疲劳(18/30)60%、高烧(17/30)56%、头痛(12/30)40% 和耐力差(11/30)36.6%。接种疫苗者的 IgG 滴度(33.067 ± 2.5)与未接种者的 IgG 滴度(23.916 ± 3.31)之间存在明显差异,P 值 = 0.025。 另一方面,人群的人口统计学特征(包括合并症、血液透析、不同年龄组、性别、感染/未感染差异)对 IgG 滴度没有统计学意义上的影响。 接种疫苗可明显提高肾移植受者的保护性 IgG 水平。关键是要重视提高肾移植受者对疫苗接种的接受度,以防止感染其他 COVID-19 变体或病原体爆发。
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引用次数: 0
Molecular Epidemiology of SARS-CoV-2 Variants in Vaccinated and Non-Vaccinated Patients of Erbil Province, Kurdistan Region-Iraq 伊拉克库尔德斯坦地区埃尔比勒省已接种和未接种疫苗的 SARS-CoV-2 变体的分子流行病学研究
Pub Date : 2024-05-03 DOI: 10.59786/bmtj.213
Dlshad Abdullah Hasan, S. Maulud, R. F. Rashid, Jivan Qasim, Rezhna Kheder, Article Info
Multiple new SARS-CoV-2 variants of concern (VOC) have emerged globally since the onset of the COVID-19 pandemic. With the virus continuing to evolve, more are expected. This emphasizes the need for rapid diagnostic methods for the detection of circulating lineages. Variants-specific real-time reverse transcription (rRT)-PCR method can be used as an alternative to genome sequencing, which is expensive and labored for identifying these variants, especially in settings with limited resources. We assessed the prevalence of various SARS-CoV-2 variants spreading in the Erbil province using a diagnostic screening RT-PCR-based method. A total of 144 SARS-CoV-2 positive samples were prospectively tested for known SARS-CoV2 variants using ViroQ® SC2 Variant rRT-PCR. Furthermore, the technique was validated using 25 SARS-CoV-2 negative nasal samples. Out of 144 SARS-CoV-2 positive samples, 118 (81.9%) were B.1.617.2 (Delta), 5 (3.5%) were Epsilon B.1.427/B.1.429, 1(0.7%) was Eta B.1.525, 2(1.4%) were SARS-CoV-2 Wild type, while 18 (12.5%) were undefined variant, and the delta strain was the most prevalent SARS-CoV-2 strain. Our study showed that variant-specific RT-PCR could be a useful tool for the rapid screening of SARS-CoV-2 variants.
自 COVID-19 大流行以来,全球出现了多个新的 SARS-CoV-2 变异株 (VOC)。随着病毒的不断进化,预计还会出现更多变种。这就强调了对快速诊断方法的需求,以检测流行的变异株。变异株特异性实时反转录(rRT)-PCR 方法可用于替代基因组测序,而基因组测序在确定这些变异株方面既昂贵又费力,尤其是在资源有限的情况下。我们采用基于 RT-PCR 的诊断筛选方法评估了埃尔比勒省传播的各种 SARS-CoV-2 变异株的流行情况。我们使用 ViroQ® SC2 变异 rRT-PCR 对 144 份 SARS-CoV-2 阳性样本进行了已知 SARS-CoV2 变异的前瞻性检测。此外,还使用 25 份 SARS-CoV-2 阴性鼻腔样本对该技术进行了验证。在 144 份 SARS-CoV-2 阳性样本中,118 份(81.9%)为 B.1.617.2(Delta),5 份(3.5%)为 Epsilon B.1.427/B.1.429,1 份(0.7%)为 Eta B.1.525,2 份(1.4%)为 SARS-CoV-2 野生型,18 份(12.5%)为未定义的变异株,Delta 株是最常见的 SARS-CoV-2 株。我们的研究表明,变异株特异性 RT-PCR 是快速筛选 SARS-CoV-2 变异株的有效工具。
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引用次数: 0
Prevalence of Asymptomatic Bacteriuria among Patients Attending Diabetic Clinic at Fort Port Regional Referral Hospital, Uganda Patients 乌干达港堡地区转诊医院糖尿病门诊患者无症状性细菌尿的患病率 患者
Pub Date : 2024-05-03 DOI: 10.59786/bmtj.216
Wardat Rashid Ali, Alina Peris, Stella Nabirye, Awil Abdi, Nihfadh Tamali, Venance Emmanuel, Dayyabu Shehu
The availability of high concentration of sugar in the blood of diabetic patients makes them more susceptible to developing bacterial infections which are asymptomatic commonly referred to as asymptomatic bacteriuria (ASB) than the non-diabetic patients. Diabetes patients with ASB have a much higher risk of developing diabetic complications than individuals without ASB. There is a paucity of data on the burden of ASB and antimicrobial susceptibility in diabetes patients in Uganda. Between March and May 2023, a cross-sectional study was carried out at the Fort Portal Regional Referral Hospital, Uganda outpatient diabetic clinic and 160 diabetes patients were recruited for the study. Mid-stream urine was taken for culture and sensitivity. Data analysis were performed using IBM SPSS version 20. A pie chart was used to display the prevalence and antimicrobial susceptibility patterns of common uropathogens described in frequency and percentages. The overall prevalence of ASB among the study participants was 11%. The most common uropathogen isolated was Escherichia coli, followed by Klebsiella and S. aureus. The most sensitive drug in all the isolates was Nitrofrantoin and resistant drug was Ampicillin. In conclusion, routine testing for ASB and treatment should be according to the antimicrobial susceptibility of diabetic patients.
与非糖尿病患者相比,糖尿病患者血液中高浓度的糖分使他们更容易发生无症状的细菌感染,通常称为无症状菌尿(ASB)。与无症状菌尿的患者相比,有无症状菌尿的糖尿病患者发生糖尿病并发症的风险要高得多。有关乌干达糖尿病患者ASB负担和抗菌药物敏感性的数据很少。2023 年 3 月至 5 月期间,一项横断面研究在乌干达波特尔堡地区转诊医院的糖尿病门诊进行,共招募了 160 名糖尿病患者参与研究。采集中段尿液进行培养和药敏试验。数据分析使用 IBM SPSS 20 版本进行。采用饼图显示常见尿路病原体的流行率和抗菌药敏感性模式,以频率和百分比表示。在研究参与者中,ASB 的总体流行率为 11%。最常见的尿路病原体是大肠埃希菌,其次是克雷伯氏菌和金黄色葡萄球菌。所有分离菌中最敏感的药物是硝基呋喃妥因,耐药药物是氨苄西林。总之,应根据糖尿病患者对抗菌药的敏感性对 ASB 进行常规检测和治疗。
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引用次数: 0
Evaluation of Melatonin and Antioxidant Levels in the Serum of Breast Cancer Patients 评估乳腺癌患者血清中的褪黑激素和抗氧化剂水平
Pub Date : 2024-05-03 DOI: 10.59786/bmtj.215
Amjad Mahmood Qadir, Arzu Karatepe, Ayşegül Yazici, R. F. Rashid, P. Koparir, R. Omer, Shamal R. Hama
Metabolites and antioxidants can be altered in patients with various diseases, particularly in those with cancer. This study aims to measure melatonin and total antioxidant levels in the serum of breast cancer patients and compare them with a healthy control group. Both groups had serum samples collected at 2:00 a.m. and 9:00 a.m. Melatonin levels were determined using High-performance liquid chromatography (HPLC), while total antioxidant levels were assessed by Enzyme-linked immunosorbent assay (ELISA). Levels of melatonin and total antioxidants differed between the groups. In the control group, the levels of melatonin and total antioxidants were considerably greater compared to the diagnostic group, with a p-value of 0.001. Melatonin and total antioxidant levels were consistently greater at 2:00 than at 9:00. The maximum melatonin levels recorded in the diagnostic and control groups were 39.3 pg/mL and 65.9 pg/mL, respectively. Melatonin levels varied based on the age of the participants, with higher amounts observed in younger participants compared to older people in both groups. The BMI affected the levels of melatonin, with a greater BMI leading to elevated melatonin concentrations. The diagnostic group had a total antioxidant level of 39.3 pg/mL, while the control group had a level of 65.9 pg/mL. The elevated levels of melatonin and total antioxidants in the control group indicate a healthy status. The research demonstrated a significant correlation between melatonin and antioxidant levels in breast cancer patients, with reduced melatonin levels and total antioxidant levels in the diagnosed group. Elevated BMI was linked to reduced melatonin levels.
各种疾病的患者,尤其是癌症患者体内的代谢物和抗氧化剂会发生变化。本研究旨在测量乳腺癌患者血清中的褪黑激素和总抗氧化剂水平,并与健康对照组进行比较。褪黑素水平采用高效液相色谱法(HPLC)测定,总抗氧化剂水平采用酶联免疫吸附法(ELISA)评估。各组的褪黑激素和总抗氧化剂水平存在差异。对照组的褪黑激素和总抗氧化剂水平明显高于诊断组,P 值为 0.001。褪黑激素和总抗氧化剂水平在 2:00 始终高于 9:00。诊断组和对照组的褪黑激素最高水平分别为 39.3 pg/mL 和 65.9 pg/mL。褪黑激素水平因参与者的年龄而异,在两组中,年轻参与者的褪黑激素水平均高于老年人。体重指数会影响褪黑激素水平,体重指数越大,褪黑激素浓度越高。诊断组的总抗氧化剂水平为 39.3 pg/mL,而对照组为 65.9 pg/mL。对照组中褪黑激素和总抗氧化剂水平的升高表明他们处于健康状态。研究表明,乳腺癌患者的褪黑激素和抗氧化剂水平之间存在明显的相关性,确诊组的褪黑激素水平和总抗氧化剂水平降低。体重指数升高与褪黑激素水平降低有关。
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引用次数: 0
Toxicological Effects of Commonly Used Mosquito Coil Smoke on Liver and Lung Function in Rats 常用蚊香烟雾对大鼠肝脏和肺功能的毒理影响
Pub Date : 2024-05-03 DOI: 10.59786/bmtj.212
Hadiza Abubakar, Yahuza Gimba Muhammed, Alhassan Muhammad Wudil, Dayyabu Shehu, Murtala Yaú
The utilization of mosquito coils as a household-level strategy for mosquito control is widely practiced in malaria-endemic regions spanning Africa, Asia, and South America. However, the continuous application of mosquito coils has prompted significant apprehensions concerning environmental and health implications. This research aims to evaluate the potential hepatic and pulmonary toxicities associated with the inhalation of mosquito coil smoke in rat subjects over both sub-chronic and chronic durations. A questionnaire was used to make inquiries regarding the prevalent types and brands of mosquito coils employed, the manner in which they are used, the durations for which they are utilized. A total of twenty-four albino rats were categorized into three distinct groups: the control group and two exposed groups. These groups were subjected to distinct brands of mosquito coil smoke across two distinct exposure regimens – a sub-chronic exposure lasting 4 weeks and a chronic exposure spanning 12 weeks. Following the exposure periods, blood samples were collected for the analysis of serum-based and biochemical markers, including serum aspartate aminotransferase (AST), alanine aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), bilirubin, serum bicarbonate, and Lactate dehydrogenase (LDH), oxygen saturation, C-reactive protein, total protein, and microscopic analysis of lung tissue and liver tissue. The histopathological analysis of lung tissue was appropriately included. However, the analysis of liver tissue was omitted. The results demonstrated a significant elevation in the levels of these biochemical markers among the exposed rats in comparison to the control group. Concurrently, a decrease in blood oxygen saturation was noted in the exposed rats when contrasted with the control group. Conversely, the serum concentrations of high-sensitivity C-reactive protein, total protein, and alanine aminotransferase (ALT) did not exhibit notable differences in relation to the control group. Additionally, microscopic analysis of lung tissue indicated the presence of histopathological lesions in the exposed rats, suggesting a detrimental impact of mosquito coil smoke exposure in a time-dependent progression.
在非洲、亚洲和南美洲的疟疾流行地区,人们广泛使用蚊香作为家庭灭蚊策略。然而,蚊香的持续应用引起了人们对环境和健康影响的极大担忧。本研究旨在评估大鼠在亚慢性和慢性吸入蚊香烟雾后可能产生的肝脏和肺部毒性。研究采用问卷调查的方式,对蚊香的普遍类型和品牌、使用方式、使用时间等进行了调查。共有二十四只白化大鼠被分为三个不同的组别:对照组和两个暴露组。这些组别在两种不同的暴露方案中接受了不同品牌的蚊香烟雾,一种是持续 4 周的亚慢性暴露,另一种是持续 12 周的慢性暴露。暴露期结束后,采集血液样本分析血清和生化指标,包括血清天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)、γ-谷氨酰转移酶(GGT)、胆红素、血清碳酸氢盐、乳酸脱氢酶(LDH)、血氧饱和度、C 反应蛋白、总蛋白,以及肺组织和肝组织的显微分析。肺组织的组织病理学分析被适当纳入。然而,肝组织的分析却被省略了。结果表明,与对照组相比,暴露大鼠的这些生化指标水平明显升高。同时,与对照组相比,暴露大鼠的血氧饱和度也有所下降。相反,高敏 C 反应蛋白、总蛋白和丙氨酸氨基转移酶(ALT)的血清浓度与对照组相比没有明显差异。此外,肺组织的显微镜分析表明,暴露于蚊香烟雾的大鼠出现了组织病理学病变,这表明暴露于蚊香烟雾会随着时间的推移而产生有害影响。
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引用次数: 0
Efficient Machine Learning and Deep Learning Techniques for Detection of Breast Cancer Tumor 用于检测乳腺癌肿瘤的高效机器学习和深度学习技术
Pub Date : 2024-05-03 DOI: 10.59786/bmtj.211
Dlshad Abdalrahman Mahmood, Sadegh Abdullah Aminfar
The detection of cancer tumors is an essential component that has important consequences for the speedy involvement of medical professionals and the enhancement of patient outcomes. This review paper presents a complete study of the current body of research and methodology, as well as an in-depth assessment of the use of machine learning (ML) and deep learning (DL) in the detection of cancer tumors. In addition, the article gives a full analysis of the approaches involved. Machine learning and deep learning, which effectively handle ambiguity in the identification of malignant tumors, provide an alternative method for dealing with the complexity of brain tissue. This method is offered by a combination of machine learning and deep learning. The first part of the review draws attention to the significance of making an accurate diagnosis of breast cancer, highlights the limits of traditional diagnostic methods, and investigates the cutting-edge area of medical imaging technology. After that, it investigates the fundamentals of ML and DL and how they might be used to deal with the challenges that are inherent in the interpretation of complicated imaging data. In addition, the paper explores the ways in which models enhance the processes of feature extraction, picture segmentation, and classification in breast tumor detection systems.
癌症肿瘤的检测是一个重要环节,对医疗专业人员的快速介入和提高患者的治疗效果有着重要影响。本综述论文对当前的研究和方法进行了全面研究,并对机器学习(ML)和深度学习(DL)在癌症肿瘤检测中的应用进行了深入评估。此外,文章还对相关方法进行了全面分析。机器学习和深度学习能有效处理恶性肿瘤识别中的模糊性,为处理脑组织的复杂性提供了另一种方法。这种方法由机器学习和深度学习相结合提供。综述的第一部分提请人们注意准确诊断乳腺癌的意义,强调了传统诊断方法的局限性,并研究了医学影像技术的前沿领域。随后,论文探讨了 ML 和 DL 的基本原理,以及如何利用它们来应对复杂成像数据解读过程中固有的挑战。此外,论文还探讨了模型如何在乳腺肿瘤检测系统中增强特征提取、图片分割和分类过程。
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引用次数: 0
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BioMed Target Journal
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