VIR 系列中根部颜色对比强烈的甜菜品种中甜菜素生物合成关键基因的等位基因差异

Q4 Biochemistry, Genetics and Molecular Biology Proceedings on Applied Botany, Genetics and Breeding Pub Date : 2024-05-03 DOI:10.30901/2227-8834-2024-1-139-151
A. Mikhailova, D. V. Sokolova, N. Shvachko, V. S. Popov, E. Khlestkina
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引用次数: 0

摘要

背景。甜菜(Beta vulgaris L.)含有大量天然红色甜菜素。由于国内植物原料储备不足,一些企业被迫进口国外染料。有鉴于此,目前需要培育甜菜苷色素含量高的栽培品种。采用 Sanger DNA 测序方法,在 VIR 收集的不同根色的甜菜品种中筛选甜菜素生物合成途径关键基因的等位基因差异。我们首次在黄肉甜菜品种'Serdolik'中发现了 CYP76AD1 基因的无义突变,它导致了 P450 功能域的截断。我们认为,检测到的多态性与表型转换有关,因为众所周知,CYP76AD1 的作用对于红色甜菜红素的积累至关重要。此外,我们还在品种 "雪崩 "的 BvDODA1 基因的第一个外显子中发现了一些错义突变。这些突变可能与不着色表型的表达有关。一项硅分析发现,CYP76AD5 的拷贝高度同源,串联在第 9 号染色体上。应注意这些拷贝以及 CYP76AD6 基因,因为它们似乎是提高红色素含量的最理想的基因敲除目标。从之前筛选出的高甜菜素群体中,我们可以找出最适合进一步进行基因组编辑的甜菜品种。此外,这项研究还揭示了甜菜苷生物合成途径关键基因的等位基因差异。这些结果将有助于开发 DNA 分子标记,以促进具有所需特性的甜菜品种的选育。
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Allelic differences in the key genes of betalain biosynthesis in table beet accessions with contrasting root color from the VIR collection
Background. Table beet (Beta vulgaris L.) contains a great amount of naturally red-colored betanins. A number of enterprises are forced to import foreign dyes because the reserves of domestic plant raw materials are insufficient. With this in view, the development of cultivars with high betalain pigment content is now required.Materials and methods. Allelic differences were screened in the key genes of the betalain biosynthesis pathway among table beet accessions with various root color from the VIR collection using the Sanger DNA sequencing method.Results and discussion. For the first time we identified a nonsense mutation in the CYP76AD1 gene in cv. ‘Serdolik’ with yellow flesh; it led to the truncation of the functional P450 domain. We suggested that the detected polymorphism correlated with phenotypic switching because the well-known role of CYP76AD1 was essential for the red betalain accumulation. Moreover, a number of missense mutations in cv. ‘Avalanche’ in the first exon of the BvDODA1 gene were found. These mutations were probably associated with the expression of the uncolored phenotype. An in silico analysis revealed highly homologous copies of CYP76AD5, located tandemly on chromosome 9. Attention should be paid to these copies, together with the CYP76AD6 gene, as they seem the most preferable targets for a knockout to increase the red pigment content.Conclusion. It is possible to identify the best table beet accessions for further genome editing among a previously selected high-betanin group. In addition, this study revealed the allelic differences in the key genes of the betalain biosynthesis pathway. These results will be useful for the development of DNA molecular markers to facilitate the selection of table beet forms with required properties.
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来源期刊
Proceedings on Applied Botany, Genetics and Breeding
Proceedings on Applied Botany, Genetics and Breeding Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
0.70
自引率
0.00%
发文量
65
审稿时长
12 weeks
期刊最新文献
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