Yuhong Bao , Tianyi Dong , Tao Shao , Wenbo Wang , Theodoros Varzakas , Sofia Agriopoulou , Xianjun Yuan
{"title":"全株玉米青贮中热带醋酸杆菌 AT7 和植物乳杆菌 LP64 的抗真菌和霉菌毒素解毒潜力","authors":"Yuhong Bao , Tianyi Dong , Tao Shao , Wenbo Wang , Theodoros Varzakas , Sofia Agriopoulou , Xianjun Yuan","doi":"10.1016/j.anifeedsci.2024.115987","DOIUrl":null,"url":null,"abstract":"<div><p>Whole-plant corn silage is an important forage for ruminant livestock; however, it is vulnerable to contamination by spoilage molds and mycotoxins because corn is an excellent substrate for fungal growth. Mycotoxins in corn silage have been found to significantly impair the productivity, health status, and fertility of dairy herds. It is urgent to develop a biological control strategy to prevent toxigenic fungi and mitigate mycotoxins in silage. This study aimed to evaluate the biocontrol potential of two novel isolated strains against toxigenic fungi and their toxins in corn silage. <em>Acetobacter tropicalis</em> AT7 and <em>Lactiplantibacillus plantarum</em> LP64 were isolated from mold-contaminated silages based on their potential antifungal and detoxifying properties. The antifungal activity of the two strains was affected by pH and heating, the antifungal compounds against <em>A. flavus</em> might be proteinaceous substances. The supernatants and cell pellets of the two isolates exhibited mycotoxin removal abilities for Aflatoxin B<sub>1</sub> (AFB<sub>1</sub>), Zearalenone (ZEN), and Deoxynivalenol (DON), indicating that both adsorption and biodegradation contributed to mycotoxin detoxification. The two strains were ensiled with artificial fungal-infected (FI) or non-fungal-infected (NFI) whole-plant corn for 180 d. The FI silages had higher concentrations of aflatoxin B<sub>1</sub> and deoxynivalenol than NFI silages. Both <em>A. tropicalis</em> AT7 and <em>L. plantarum</em> LP64 decreased aflatoxin B<sub>1</sub> and zearalenone concentrations. Two strains affected the bacterial and fungal communities during the ensiling, indicated by the decline in the relative abundance (RA) of <em>Pseudopithomyces</em> and <em>Periconia</em> as compared to untreated corn silages. Inoculating <em>A. tropicalis</em> AT7 increased microbial network stability of NFI silages. In summary, <em>A. tropicalis</em> AT7 has the potential to be used as a biological preservative for reducing mycotoxigenic fungi and mycotoxin contamination in silages.</p></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Antifungal and mycotoxin detoxification potentials of Acetobacter tropicalis AT7 and Lactiplantibacillus plantarum LP64 in whole-plant corn silage\",\"authors\":\"Yuhong Bao , Tianyi Dong , Tao Shao , Wenbo Wang , Theodoros Varzakas , Sofia Agriopoulou , Xianjun Yuan\",\"doi\":\"10.1016/j.anifeedsci.2024.115987\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Whole-plant corn silage is an important forage for ruminant livestock; however, it is vulnerable to contamination by spoilage molds and mycotoxins because corn is an excellent substrate for fungal growth. Mycotoxins in corn silage have been found to significantly impair the productivity, health status, and fertility of dairy herds. It is urgent to develop a biological control strategy to prevent toxigenic fungi and mitigate mycotoxins in silage. This study aimed to evaluate the biocontrol potential of two novel isolated strains against toxigenic fungi and their toxins in corn silage. <em>Acetobacter tropicalis</em> AT7 and <em>Lactiplantibacillus plantarum</em> LP64 were isolated from mold-contaminated silages based on their potential antifungal and detoxifying properties. The antifungal activity of the two strains was affected by pH and heating, the antifungal compounds against <em>A. flavus</em> might be proteinaceous substances. The supernatants and cell pellets of the two isolates exhibited mycotoxin removal abilities for Aflatoxin B<sub>1</sub> (AFB<sub>1</sub>), Zearalenone (ZEN), and Deoxynivalenol (DON), indicating that both adsorption and biodegradation contributed to mycotoxin detoxification. The two strains were ensiled with artificial fungal-infected (FI) or non-fungal-infected (NFI) whole-plant corn for 180 d. The FI silages had higher concentrations of aflatoxin B<sub>1</sub> and deoxynivalenol than NFI silages. Both <em>A. tropicalis</em> AT7 and <em>L. plantarum</em> LP64 decreased aflatoxin B<sub>1</sub> and zearalenone concentrations. Two strains affected the bacterial and fungal communities during the ensiling, indicated by the decline in the relative abundance (RA) of <em>Pseudopithomyces</em> and <em>Periconia</em> as compared to untreated corn silages. Inoculating <em>A. tropicalis</em> AT7 increased microbial network stability of NFI silages. In summary, <em>A. tropicalis</em> AT7 has the potential to be used as a biological preservative for reducing mycotoxigenic fungi and mycotoxin contamination in silages.</p></div>\",\"PeriodicalId\":7861,\"journal\":{\"name\":\"Animal Feed Science and Technology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2024-05-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Animal Feed Science and Technology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0377840124001159\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animal Feed Science and Technology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0377840124001159","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
Antifungal and mycotoxin detoxification potentials of Acetobacter tropicalis AT7 and Lactiplantibacillus plantarum LP64 in whole-plant corn silage
Whole-plant corn silage is an important forage for ruminant livestock; however, it is vulnerable to contamination by spoilage molds and mycotoxins because corn is an excellent substrate for fungal growth. Mycotoxins in corn silage have been found to significantly impair the productivity, health status, and fertility of dairy herds. It is urgent to develop a biological control strategy to prevent toxigenic fungi and mitigate mycotoxins in silage. This study aimed to evaluate the biocontrol potential of two novel isolated strains against toxigenic fungi and their toxins in corn silage. Acetobacter tropicalis AT7 and Lactiplantibacillus plantarum LP64 were isolated from mold-contaminated silages based on their potential antifungal and detoxifying properties. The antifungal activity of the two strains was affected by pH and heating, the antifungal compounds against A. flavus might be proteinaceous substances. The supernatants and cell pellets of the two isolates exhibited mycotoxin removal abilities for Aflatoxin B1 (AFB1), Zearalenone (ZEN), and Deoxynivalenol (DON), indicating that both adsorption and biodegradation contributed to mycotoxin detoxification. The two strains were ensiled with artificial fungal-infected (FI) or non-fungal-infected (NFI) whole-plant corn for 180 d. The FI silages had higher concentrations of aflatoxin B1 and deoxynivalenol than NFI silages. Both A. tropicalis AT7 and L. plantarum LP64 decreased aflatoxin B1 and zearalenone concentrations. Two strains affected the bacterial and fungal communities during the ensiling, indicated by the decline in the relative abundance (RA) of Pseudopithomyces and Periconia as compared to untreated corn silages. Inoculating A. tropicalis AT7 increased microbial network stability of NFI silages. In summary, A. tropicalis AT7 has the potential to be used as a biological preservative for reducing mycotoxigenic fungi and mycotoxin contamination in silages.
期刊介绍：
Animal Feed Science and Technology is a unique journal publishing scientific papers of international interest focusing on animal feeds and their feeding.
Papers describing research on feed for ruminants and non-ruminants, including poultry, horses, companion animals and aquatic animals, are welcome.
The journal covers the following areas:
Nutritive value of feeds (e.g., assessment, improvement)
Methods of conserving and processing feeds that affect their nutritional value
Agronomic and climatic factors influencing the nutritive value of feeds
Utilization of feeds and the improvement of such
Metabolic, production, reproduction and health responses, as well as potential environmental impacts, of diet inputs and feed technologies (e.g., feeds, feed additives, feed components, mycotoxins)
Mathematical models relating directly to animal-feed interactions
Analytical and experimental methods for feed evaluation
Environmental impacts of feed technologies in animal production.
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