LncRNA 心肌梗死相关转录本 (MIAT)/miR-505-5p 轴调控高血压小鼠血管平滑肌细胞的增殖和迁移

IF 2.9 4区 医学 Q1 Medicine Journal of biomedical nanotechnology Pub Date : 2024-05-01 DOI:10.1166/jbn.2024.3824
Hua Yang, Hongfei Song, Pan Xiong
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引用次数: 0

摘要

目的:阐明长非编码 RNA(lncRNA)MIAT 通过下调 microRNA-505-5p (miR-505-5p)调节高血压小鼠血管内皮细胞增殖和迁移能力的作用。检测了 20 名高血压患者和 20 名健康志愿者血清中 MIAT 和 miR-505-5p 的水平。提取了高血压小鼠和健康小鼠的血管内皮细胞。研究了 MIAT 和 miR-505-5p 对血管内皮细胞增殖和迁移能力的调控作用。最后,通过双荧光素酶报告实验和拯救实验探讨了 MIAT 和 miR-505-5p 之间的相互作用。结果发现,高血压患者血清中的 MIAT 水平高于健康人,而 miR-505-5p 则出现下调。高血压患者血清中 MIAT 的水平与 miR-505-5p 的水平呈负相关。敲除 MIAT 可抑制高血压小鼠血管内皮细胞的增殖和迁移能力。此外,敲除 MIAT 会上调 E-cadherin,下调 Vimentin 和 Snail-1。MiR-505-5p 被证实是结合 MIAT 的靶标。敲除 miR-505-5p 逆转了 MIAT 对 VSMC 表型的调控作用。LncRNA MIAT通过下调miR-505-5p刺激高血压小鼠的VSMC增殖和迁移,这可能是诊断和治疗高血压的一个有前景的靶点。
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LncRNA Myocardial Infarction-Associated Transcript (MIAT)/miR-505-5p Axis Regulates Proliferation and Migration of Vascular Smooth Muscle Cells of Hypertension Mice
To clarify the role of long non-coding RNA (lncRNA) MIAT in regulating proliferative and migratory abilities in VSMCs extracted from hypertension mice via downregulating microRNA-505-5p (miR-505-5p). Serum levels of MIAT and miR-505-5p in enrolled 20 hypertension patients and 20 healthy volunteers were detected. VSMCs were extracted from hypertension mice and healthy mice. Regulatory effects of MIAT and miR-505-5p on proliferative and migratory abilities in VSMCs were examined. At last, the interaction between MIAT and miR-505-5p was explored by dual-luciferase reporter assay and rescue experiments. Serum level of MIAT was higher in hypertension patients than those of healthy subjects, while miR-505-5p was downregulated. MIAT level was negatively correlated to miR-505-5p level in serum of hypertension patients. Knockdown of MIAT suppressed proliferative and migratory abilities in VSMCs extracted from hypertension mice. In addition, knockdown of MIAT upregulated E-cadherin and downregulated Vimentin and Snail-1. MiR-505-5p was verified to be the target binding MIAT. Knockdown of miR-505-5p reversed regulatory effects of MIAT on VSMCs phenotypes. LncRNA MIAT stimulates VSMCs in hypertension mice to proliferate and migrate through downregulating miR-505-5p, which may be a promising target for diagnosis and treatment of hypertension.
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CiteScore
4.30
自引率
17.20%
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145
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2.3 months
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