SERPING1 基因单核苷酸多态性与喀拉拉邦热带潮湿地区本地山羊对天然强疟原虫感染的抵抗力的关系

IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Small Ruminant Research Pub Date : 2024-05-14 DOI:10.1016/j.smallrumres.2024.107288
Reshmi Raveendran , Syamala K , Marykutty Thomas , Bindu Lakshmanan , Asha Rajagopal
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引用次数: 0

摘要

强直性脊柱炎对山羊种群构成严重威胁,尤其是在印度南部半岛喀拉拉邦等潮湿的热带地区。本研究旨在比较在半集约化饲养系统下饲养的阿塔帕迪黑山羊和马拉巴里山羊在宿主抵抗力和对自然强疟原虫感染的恢复力方面的品种差异。此外,我们还分析了丝氨酸蛋白酶抑制剂 G 族成员 1(SERPING1)基因中潜在的单核苷酸多态性(SNP)与这些性状的关联。粪蛋计数(FEC)和包装红细胞体积(VPRC)分别被用作宿主抵抗力和恢复力的指标。在一年的时间里,每两周从 109 只本地山羊(包括 58 只马拉巴里山羊和 51 只阿塔帕迪黑山羊)身上收集粪便卵数和红细胞体积的表型数据。与阿塔帕迪黑山羊相比,马拉巴里山羊对强直性脊柱炎表现出更强的抵抗力和复原力,表现为明显较低的FEC(p≤0.01)和较高的VPRC(p≤0.05)。皮尔逊相关分析表明,FEC 与 VPRC 之间存在着非常明显的负相关(r= -0.41,p<0.01)。利用改良的特定等位基因双向 PCR 扩增(Bi-PASA)技术对 SERPING1 基因中的 -312-C>T SNP 进行了基因分型。虽然 SERPING1 基因型与 FEC 之间的关联不显著,但观察到 SERPING1 基因型与 VPRC 之间存在显著关联(p<0.05)。这些发现强调了山羊对强疟原虫感染的抗病力存在品种特异性差异的重要性,并突出了 SERPING1 基因多态性在调节宿主抗病力特征方面的潜在作用。
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Association of SERPING1 gene single nucleotide polymorphism with resistance to natural strongyle infection among native goats in humid tropical Kerala

Strongylosis poses a significant threat to goat populations, particularly in humid tropical regions like Kerala, a southern peninsular state in India. This study aimed to compare breed differences in host resistance and resilience to natural strongyle infection between Attappady Black and Malabari goats reared under semi-intensive systems. Additionally, we analyzed the association of a potential single nucleotide polymorphism (SNP) in the Serine Proteinase Inhibitor Clade G member 1 (SERPING1) gene with these traits. Faecal egg count (FEC) and volume of packed red cells (VPRC) were employed as indicators of host resistance and resilience, respectively. Over the course of one year, fortnightly phenotypic data on FEC and VPRC were collected from 109 native goats, including 58 Malabari and 51 Attappady Black goats. The Malabari goats exhibited greater resistance and resilience to strongylosis, evidenced by significantly lower FEC (p≤0.01) and higher VPRC (p≤0.05) compared to Attappady Black goats. Pearson correlation analysis revealed a highly significant negative correlation between FEC and VPRC (r= −0.41, p<0.01). Genotyping of the −312-C>T SNP in SERPING1 gene was conducted using modified bidirectional PCR amplification of specific alleles (Bi-PASA). While the association between SERPING1 genotypes and FEC was not significant, a significant association was observed between SERPING1 genotypes and VPRC (p<0.05). These findings underscore the importance of breed-specific differences in goat resilience to strongyle infection and highlight the potential role of SERPING1 gene polymorphism in modulating host resilience traits.

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来源期刊
Small Ruminant Research
Small Ruminant Research 农林科学-奶制品与动物科学
CiteScore
3.10
自引率
11.10%
发文量
210
审稿时长
12.5 weeks
期刊介绍: Small Ruminant Research publishes original, basic and applied research articles, technical notes, and review articles on research relating to goats, sheep, deer, the New World camelids llama, alpaca, vicuna and guanaco, and the Old World camels. Topics covered include nutrition, physiology, anatomy, genetics, microbiology, ethology, product technology, socio-economics, management, sustainability and environment, veterinary medicine and husbandry engineering.
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