细胞结构生物学:注意概念化与实现之间的差距

IF 6.1 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Current opinion in structural biology Pub Date : 2024-05-23 DOI:10.1016/j.sbi.2024.102843
Fotis L. Kyrilis , Jason K.K. Low , Joel P. Mackay , Panagiotis L. Kastritis
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引用次数: 0

摘要

最近的技术进步加深了我们对细胞结构的认识。然而,大多数结构数据并非来自完整的细胞,这限制了我们对细胞过程的理解。在此,我们将讨论当前和未来的发展,这些发展将为我们带来细胞结构图。电子冷冻成像技术是标准的承载者,它能够提供细胞内快照。单颗粒电子显微镜(纯化的生物分子和复杂的混合物)和共价交联结合质谱法也能发挥重要作用,还有各种形式的人工智能算法。要整合这些多种方法,数据整理和标准化将至关重要--同样重要的是,我们需要将工作范围从目前以蛋白质为中心的视角扩展到维持生命的其他(宏)分子。
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Structural biology in cellulo: Minding the gap between conceptualization and realization

Recent technological advances have deepened our perception of cellular structure. However, most structural data doesn't originate from intact cells, limiting our understanding of cellular processes. Here, we discuss current and future developments that will bring us towards a structural picture of the cell. Electron cryotomography is the standard bearer, with its ability to provide in cellulo snapshots. Single-particle electron microscopy (of purified biomolecules and of complex mixtures) and covalent crosslinking combined with mass spectrometry also have significant roles to play, as do artificial intelligence algorithms in their many guises. To integrate these multiple approaches, data curation and standardisation will be critical – as is the need to expand efforts beyond our current protein-centric view to the other (macro)molecules that sustain life.

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来源期刊
Current opinion in structural biology
Current opinion in structural biology 生物-生化与分子生物学
CiteScore
12.20
自引率
2.90%
发文量
179
审稿时长
6-12 weeks
期刊介绍: Current Opinion in Structural Biology (COSB) aims to stimulate scientifically grounded, interdisciplinary, multi-scale debate and exchange of ideas. It contains polished, concise and timely reviews and opinions, with particular emphasis on those articles published in the past two years. In addition to describing recent trends, the authors are encouraged to give their subjective opinion of the topics discussed. In COSB, we help the reader by providing in a systematic manner: 1. The views of experts on current advances in their field in a clear and readable form. 2. Evaluations of the most interesting papers, annotated by experts, from the great wealth of original publications. [...] The subject of Structural Biology is divided into twelve themed sections, each of which is reviewed once a year. Each issue contains two sections, and the amount of space devoted to each section is related to its importance. -Folding and Binding- Nucleic acids and their protein complexes- Macromolecular Machines- Theory and Simulation- Sequences and Topology- New constructs and expression of proteins- Membranes- Engineering and Design- Carbohydrate-protein interactions and glycosylation- Biophysical and molecular biological methods- Multi-protein assemblies in signalling- Catalysis and Regulation
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