对市中心雨水收集区进行的径流微生物质量评估显示,病原体负荷随人类流动模式的不同而有所差异。

IF 4.5 2区 医学 Q1 INFECTIOUS DISEASES International journal of hygiene and environmental health Pub Date : 2024-05-22 DOI:10.1016/j.ijheh.2024.114391
Rayan Bouchali , Claire Mandon , Emmanuelle Danty - Berger , Alain Géloën , Laurence Marjolet , Benjamin Youenou , Adrien C.M. Pozzi , Sophie Vareilles , Wessam Galia , Gislain Lipeme Kouyi , Jean-Yves Toussaint , Benoit Cournoyer
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引用次数: 0

摘要

城市地面的卫生质量可能会受到多种微生物污染物来源的影响。这些地面会引发多种细菌类群的滋生,并在降雨时通过径流水的循环促进其传播。这些径流水通常被引入下水道网络、雨水渗透系统或滞留池,然后再排放到自然水道中。随着水资源短缺成为世界性的重大问题,这些径流水成为街道清洁和植物浇灌等某些用途的替代水源。与这些城市径流相关的微生物危害和监测准则必须加以界定,以有利于这些用途。通过经典的粪便指示菌(FIB)检测、定量 PCR 和 DNA 元条码分析,对最近实施的市中心雨水收集区的径流微生物质量进行了评估。研究还调查了社会城市化模式对这些城市微生物组组织的影响。在大多数径流中都检测到了人类特异性类杆菌和病原体(如金黄色葡萄球菌)的 FIB 和 DNA,并显示出广泛的分布模式。16S rRNA DNA 元条形码分析进一步确定了引起健康关注的核心重复类群,如醋酸杆菌、分枝杆菌、气单胞菌和假单胞菌,并根据社会城市化模式的两大类群划分了这些群落。其中一组受到繁忙交通的严重影响,并显示出涉及细菌碳氢化合物降解器的重复相关网络,这些降解器具有显著的毒力特性。基于 tpm 的元条码方法在物种水平上识别出了其中的 30 多个属。在这些类群中,记录了铜绿假单胞菌、副铜绿假单胞菌和鱼腥单胞菌等反复出现的病原体。铜绿假单胞菌和鱼腥酵母菌的 tpm 读数在研究地点均匀分布,但副铜绿假单胞菌的 tpm 读数在受交通繁忙影响的子流域较高。与这些径流副绿原酸杆菌新病原体相关的健康风险很高,并且对 A549 肺细胞具有很强的细胞毒性。径流水体中病原体的反复检测突出表明,在允许使用径流水体之前,有必要对其进行微生物监测。某些具有良好卫生习惯的子流域可以获得良好的微生物质量,但并非所有子流域都是如此。人类流动性和行为的重组很可能会引发这些细菌多样性模式的变化,并减少最有害菌群的出现。
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Runoff microbiome quality assessment of a city center rainwater harvesting zone shows a differentiation of pathogen loads according to human mobility patterns

The hygienic quality of urban surfaces can be impaired by multiple sources of microbiological contaminants. These surfaces can trigger the development of multiple bacterial taxa and favor their spread during rain events through the circulation of runoff waters. These runoff waters are commonly directed toward sewer networks, stormwater infiltration systems or detention tanks prior a release into natural water ways. With water scarcity becoming a major worldwide issue, these runoffs are representing an alternative supply for some usage like street cleaning and plant watering. Microbiological hazards associated with these urban runoffs, and surveillance guidelines must be defined to favor these uses. Runoff microbiological quality from a recently implemented city center rainwater harvesting zone was evaluated through classical fecal indicator bacteria (FIB) assays, quantitative PCR and DNA meta-barcoding analyses. The incidence of socio-urbanistic patterns on the organization of these urban microbiomes were investigated. FIB and DNA from Human-specific Bacteroidales and pathogens such as Staphylococcus aureus were detected from most runoffs and showed broad distribution patterns. 16S rRNA DNA meta-barcoding profilings further identified core recurrent taxa of health concerns like Acinetobacter, Mycobacterium, Aeromonas and Pseudomonas, and divided these communities according to two main groups of socio-urbanistic patterns. One of these was highly impacted by heavy traffic, and showed recurrent correlation networks involving bacterial hydrocarbon degraders harboring significant virulence properties. The tpm-based meta-barcoding approach identified some of these taxa at the species level for more than 30 genera. Among these, recurrent pathogens were recorded such as P. aeruginosa, P. paraeruginosa, and Aeromonas caviae. P. aeruginosa and A. caviae tpm reads were found evenly distributed over the study site but those of P. paraeruginosa were higher among sub-catchments impacted by heavy traffic. Health risks associated with these runoff P. paraeruginosa emerging pathogens were high and associated with strong cytotoxicity on A549 lung cells. Recurrent detections of pathogens in runoff waters highlight the need of a microbiological surveillance prior allowing their use. Good microbiological quality can be obtained for certain typologies of sub-catchments with good hygienic practices but not all. A reorganization of Human mobility and behaviors would likely trigger changes in these bacterial diversity patterns and reduce the occurrences of the most hazardous groups.

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来源期刊
CiteScore
11.50
自引率
5.00%
发文量
151
审稿时长
22 days
期刊介绍: The International Journal of Hygiene and Environmental Health serves as a multidisciplinary forum for original reports on exposure assessment and the reactions to and consequences of human exposure to the biological, chemical, and physical environment. Research reports, short communications, reviews, scientific comments, technical notes, and editorials will be peer-reviewed before acceptance for publication. Priority will be given to articles on epidemiological aspects of environmental toxicology, health risk assessments, susceptible (sub) populations, sanitation and clean water, human biomonitoring, environmental medicine, and public health aspects of exposure-related outcomes.
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