多菌种细菌入侵人类宿主细胞。

IF 2.7 4区 医学 Q3 IMMUNOLOGY Pathogens and disease Pub Date : 2024-02-07 DOI:10.1093/femspd/ftae012
Charlotte Abell-King, Alaska Pokhrel, Scott A Rice, Iain G Duggin, Bill Söderström
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引用次数: 0

摘要

尿路感染(UTI)是全球最常见的细菌感染之一,是一种典型的多微生物感染。虽然人们从宏观上了解了整个感染过程,但在细胞水平上对细菌的行为还不完全了解,多菌种感染过程中细菌的病理生理学特征也不十分明确。在这里,我们利用临床相关细菌、人类膀胱上皮细胞和人类尿液,结合高分辨率成像技术建立了共同感染模型,以比较三种常见泌尿病原体(致病性大肠杆菌(UPEC)、肺炎克雷伯菌和粪肠球菌)的单菌种和多菌种膀胱细胞侵袭事件。虽然这三种病原体都会侵入膀胱细胞,但在流动条件下,革兰氏阳性的粪肠球菌与革兰氏阴性的尿路致病性大肠杆菌和肺炎克雷伯菌相比,侵入性明显较低。在感染实验中,如果同时引入三种细菌,它们有时会侵入同一个膀胱细胞,但侵入频率不同,这表明细菌种类与膀胱细胞之间存在复杂的相互作用。在宿主细胞内,我们观察到粪肠球菌菌落专门被 UPEC 包囊。在随后离开宿主细胞的过程中,只有革兰氏阴性细菌发生了感染相关丝状化(IRF)。总之,我们的数据表明,细菌多菌种入侵单个膀胱细胞的现象很常见,并支持了早先的研究,这些研究显示了UTI期间菌种间在生化水平上的合作。
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Multispecies bacterial invasion of human host cells.

Urinary tract infection (UTI), one of the most common bacterial infections worldwide, is a typical example of an infection that is often polymicrobial in nature. While the overall infection course is known on a macroscale, bacterial behavior is not fully understood at the cellular level and bacterial pathophysiology during multispecies infection is not well characterized. Here, using clinically relevant bacteria, human epithelial bladder cells and human urine, we establish co-infection models combined with high resolution imaging to compare single- and multi-species bladder cell invasion events in three common uropathogens: uropathogenic Escherichia coli (UPEC), Klebsiella pneumoniae and Enterococcus faecalis. While all three species invaded the bladder cells, under flow conditions the Gram-positive E. faecalis was significantly less invasive compared to the Gram-negative UPEC and K. pneumoniae. When introduced simultaneously during an infection experiment, all three bacterial species sometimes invaded the same bladder cell, at differing frequencies suggesting complex interactions between bacterial species and bladder cells. Inside host cells, we observed encasement of E. faecalis colonies specifically by UPEC. During subsequent dispersal from the host cells, only the Gram-negative bacteria underwent infection-related filamentation (IRF). Taken together, our data suggest that bacterial multispecies invasions of single bladder cells are frequent and support earlier studies showing intraspecies cooperation on a biochemical level during UTI.

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来源期刊
Pathogens and disease
Pathogens and disease IMMUNOLOGY-INFECTIOUS DISEASES
CiteScore
7.40
自引率
3.00%
发文量
44
期刊介绍: Pathogens and Disease publishes outstanding primary research on hypothesis- and discovery-driven studies on pathogens, host-pathogen interactions, host response to infection and their molecular and cellular correlates. It covers all pathogens – eukaryotes, prokaryotes, and viruses – and includes zoonotic pathogens and experimental translational applications.
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