E.B. Sobotik , K. Russo , S.P. Lerner , D. Sandvang , A. Meuter , H. McBride , R. Sayed , G. Girgis
{"title":"短讯:基于三株芽孢杆菌的商品益生菌对商品蛋鸡肠炎沙门氏菌盲肠定植的影响","authors":"E.B. Sobotik , K. Russo , S.P. Lerner , D. Sandvang , A. Meuter , H. McBride , R. Sayed , G. Girgis","doi":"10.1016/j.vas.2024.100362","DOIUrl":null,"url":null,"abstract":"<div><p>A commercial triple-strain <em>Bacillus</em>-based probiotic was tested to determine its effect on the colonization of the ceca by <em>Salmonella</em> Enteritidis (SE) in commercial layer pullets. Two treatments were tested, each with containing 128 day-of-hatch LSL layer chicks. On top of a standard diet: 1) no supplement (Control, CON), and 2) Probiotic (GalliPro® Fit, 500 g/MT, 1.6 × 10<sup>6</sup> CFU/g of finished feed, PRO). Environmental swabs were collected from each treatment group and tested to ensure freedom from SE prior to challenge. At 21 days of age, the SE challenge strain was administered orally at a dose of 3.3 × 10<sup>8</sup> CFU/bird. Pullets from each treatment group (n=32) were euthanized at 6-, 10-, 14-, and 18-days post infection (dpi). Contents from the ceca were aseptically collected and assessed for presence and abundance of SE. No differences in the prevalence of SE positive ceca following oral inoculation (<em>P</em>>0.05) were observed between treatment groups at 6-, 10-, 14-, or 18-dpi. Counts of SE in the ceca of the PRO group were not significantly different (<em>P</em>>0.05) from those of CON at 6- or 10-dpi. However, significantly lower counts of SE in the ceca of the PRO group were observed at 14-dpi (<em>P</em><0.05) and 18-dpi (<em>P</em><0.05) compared with CON. SE counts were 1.24 and 1.34 logs lower than CON at 14- and 18-dpi, respectively. In conclusion, supplementation of the triple-strain <em>Bacillus</em>-based probiotic resulted in lower cecal counts of SE compared to those that did not receive an effective probiotic, thereby reducing the risk of foodborne pathogens prior to harvest through sustainable, natural methods.</p></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":null,"pages":null},"PeriodicalIF":1.9000,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2451943X24000292/pdfft?md5=52816f3019762a49f4446487f17a7376&pid=1-s2.0-S2451943X24000292-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Short communication: Effects of a commercial triple-strain Bacillus-based probiotic on cecal colonization with Salmonella Enteritidis in commercial layer pullets\",\"authors\":\"E.B. Sobotik , K. Russo , S.P. Lerner , D. Sandvang , A. Meuter , H. McBride , R. Sayed , G. Girgis\",\"doi\":\"10.1016/j.vas.2024.100362\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A commercial triple-strain <em>Bacillus</em>-based probiotic was tested to determine its effect on the colonization of the ceca by <em>Salmonella</em> Enteritidis (SE) in commercial layer pullets. Two treatments were tested, each with containing 128 day-of-hatch LSL layer chicks. On top of a standard diet: 1) no supplement (Control, CON), and 2) Probiotic (GalliPro® Fit, 500 g/MT, 1.6 × 10<sup>6</sup> CFU/g of finished feed, PRO). Environmental swabs were collected from each treatment group and tested to ensure freedom from SE prior to challenge. At 21 days of age, the SE challenge strain was administered orally at a dose of 3.3 × 10<sup>8</sup> CFU/bird. Pullets from each treatment group (n=32) were euthanized at 6-, 10-, 14-, and 18-days post infection (dpi). Contents from the ceca were aseptically collected and assessed for presence and abundance of SE. No differences in the prevalence of SE positive ceca following oral inoculation (<em>P</em>>0.05) were observed between treatment groups at 6-, 10-, 14-, or 18-dpi. Counts of SE in the ceca of the PRO group were not significantly different (<em>P</em>>0.05) from those of CON at 6- or 10-dpi. However, significantly lower counts of SE in the ceca of the PRO group were observed at 14-dpi (<em>P</em><0.05) and 18-dpi (<em>P</em><0.05) compared with CON. SE counts were 1.24 and 1.34 logs lower than CON at 14- and 18-dpi, respectively. In conclusion, supplementation of the triple-strain <em>Bacillus</em>-based probiotic resulted in lower cecal counts of SE compared to those that did not receive an effective probiotic, thereby reducing the risk of foodborne pathogens prior to harvest through sustainable, natural methods.</p></div>\",\"PeriodicalId\":37152,\"journal\":{\"name\":\"Veterinary and Animal Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2024-05-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2451943X24000292/pdfft?md5=52816f3019762a49f4446487f17a7376&pid=1-s2.0-S2451943X24000292-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary and Animal Science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2451943X24000292\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary and Animal Science","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2451943X24000292","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
引用次数: 0
摘要
我们对一种基于三株芽孢杆菌的商用益生菌进行了试验,以确定其对商用蛋鸡肠炎沙门氏菌(SE)在盲肠定植的影响。试验采用了两种处理方法,每种处理都含有 128 只孵化一天的 LSL 蛋鸡。在标准日粮的基础上1)不添加补充剂(对照组,CON);2)添加益生菌(GalliPro® Fit,500 克/公吨,1.6 × 106 CFU/克成品饲料,PRO)。从每个处理组收集环境拭子并进行检测,以确保在挑战之前没有 SE。21 日龄时,按 3.3 × 108 CFU/只的剂量口服 SE 挑战菌株。在感染后 6、10、14 和 18 天(dpi)对各处理组(n=32)的幼鸟实施安乐死。无菌收集盲肠内容物,并评估 SE 的存在和丰度。在口服接种后 6、10、14 和 18 dpi,各处理组之间 SE 阳性盲囊的发生率无差异(P>0.05)。在6-或10-dpi时,PRO组盲肠中的SE计数与CON组无明显差异(P>0.05)。然而,在14dpi(P<0.05)和18dpi(P<0.05)时,PRO组盲肠中的SE计数明显低于CON组。在 14 dpi 和 18 dpi,SE 数量分别比 CON 低 1.24 和 1.34 logs。总之,与未接受有效益生菌的动物相比,补充三株芽孢杆菌益生菌可降低 SE 的盲肠计数,从而通过可持续的自然方法降低收获前食源性病原体的风险。
Short communication: Effects of a commercial triple-strain Bacillus-based probiotic on cecal colonization with Salmonella Enteritidis in commercial layer pullets
A commercial triple-strain Bacillus-based probiotic was tested to determine its effect on the colonization of the ceca by Salmonella Enteritidis (SE) in commercial layer pullets. Two treatments were tested, each with containing 128 day-of-hatch LSL layer chicks. On top of a standard diet: 1) no supplement (Control, CON), and 2) Probiotic (GalliPro® Fit, 500 g/MT, 1.6 × 106 CFU/g of finished feed, PRO). Environmental swabs were collected from each treatment group and tested to ensure freedom from SE prior to challenge. At 21 days of age, the SE challenge strain was administered orally at a dose of 3.3 × 108 CFU/bird. Pullets from each treatment group (n=32) were euthanized at 6-, 10-, 14-, and 18-days post infection (dpi). Contents from the ceca were aseptically collected and assessed for presence and abundance of SE. No differences in the prevalence of SE positive ceca following oral inoculation (P>0.05) were observed between treatment groups at 6-, 10-, 14-, or 18-dpi. Counts of SE in the ceca of the PRO group were not significantly different (P>0.05) from those of CON at 6- or 10-dpi. However, significantly lower counts of SE in the ceca of the PRO group were observed at 14-dpi (P<0.05) and 18-dpi (P<0.05) compared with CON. SE counts were 1.24 and 1.34 logs lower than CON at 14- and 18-dpi, respectively. In conclusion, supplementation of the triple-strain Bacillus-based probiotic resulted in lower cecal counts of SE compared to those that did not receive an effective probiotic, thereby reducing the risk of foodborne pathogens prior to harvest through sustainable, natural methods.