Gera Dinesh, K. Ramesh, Gautami S Penmetsa, P. Swetha, NVS Sruthima G, P. M. Kumar
{"title":"假牙修复后健康人和糖尿病人种植体周围的微生物学和免疫学评估:前瞻性病例对照研究","authors":"Gera Dinesh, K. Ramesh, Gautami S Penmetsa, P. Swetha, NVS Sruthima G, P. M. Kumar","doi":"10.1177/23202068241242626","DOIUrl":null,"url":null,"abstract":"Microbial colonization contributes to periodontitis and peri-implantitis with an increase in proinflammatory cytokines like IL 17. This has a vital role in the destructive process of tissue and bone around the implant. Type 2 diabetes mellitus has the potential to increase these microbial colonies thus becoming a risk factor. To compare and correlate the association of peri-implant sulcular microflora and salivary IL-17 between healthy and controlled diabetic individuals. A total of 30 participants who have undergone dental implant placement with screw-retained prosthesis were included in the study. Participants were divided into two groups, healthy ( n = 15) and diabetic ( n = 15). The subgingival plaque was collected from peri-implant sites into TE buffer vials and the salivary sample was collected into Eppendorf tubes at the 7th, 14th, and 30th day. Microbiological counts of P. gingivalis, T. denticola, T .forsythia, and F. nucleatum were evaluated by polymerase chain reaction, and salivary interleukin (IL)-17 levels were obtained by enzyme-linked immunosorbent assay. The bacterial count was increased in both healthy and diabetic individuals but more prevalent in diabetes. The salivary IL-17 levels were increased in diabetic individuals which was statistically significant. Increased microbiota in both healthy and diabetic individuals along with increased IL-17 levels can lead to peri-implantitis which provides an insight into microbiological and immunological aspects in individuals with dental implants further acting as a predictor for peri-implantitis.","PeriodicalId":43017,"journal":{"name":"Journal of Advanced Oral Research","volume":null,"pages":null},"PeriodicalIF":0.6000,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Microbiological and Immunological Assessment of Peri-implant Sites in Healthy and Diabetic Individuals After Prosthetic Rehabilitation: A Prospective Case Control Study\",\"authors\":\"Gera Dinesh, K. Ramesh, Gautami S Penmetsa, P. Swetha, NVS Sruthima G, P. M. Kumar\",\"doi\":\"10.1177/23202068241242626\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Microbial colonization contributes to periodontitis and peri-implantitis with an increase in proinflammatory cytokines like IL 17. This has a vital role in the destructive process of tissue and bone around the implant. Type 2 diabetes mellitus has the potential to increase these microbial colonies thus becoming a risk factor. To compare and correlate the association of peri-implant sulcular microflora and salivary IL-17 between healthy and controlled diabetic individuals. A total of 30 participants who have undergone dental implant placement with screw-retained prosthesis were included in the study. Participants were divided into two groups, healthy ( n = 15) and diabetic ( n = 15). The subgingival plaque was collected from peri-implant sites into TE buffer vials and the salivary sample was collected into Eppendorf tubes at the 7th, 14th, and 30th day. Microbiological counts of P. gingivalis, T. denticola, T .forsythia, and F. nucleatum were evaluated by polymerase chain reaction, and salivary interleukin (IL)-17 levels were obtained by enzyme-linked immunosorbent assay. The bacterial count was increased in both healthy and diabetic individuals but more prevalent in diabetes. The salivary IL-17 levels were increased in diabetic individuals which was statistically significant. Increased microbiota in both healthy and diabetic individuals along with increased IL-17 levels can lead to peri-implantitis which provides an insight into microbiological and immunological aspects in individuals with dental implants further acting as a predictor for peri-implantitis.\",\"PeriodicalId\":43017,\"journal\":{\"name\":\"Journal of Advanced Oral Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.6000,\"publicationDate\":\"2024-05-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Advanced Oral Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1177/23202068241242626\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Advanced Oral Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1177/23202068241242626","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Microbiological and Immunological Assessment of Peri-implant Sites in Healthy and Diabetic Individuals After Prosthetic Rehabilitation: A Prospective Case Control Study
Microbial colonization contributes to periodontitis and peri-implantitis with an increase in proinflammatory cytokines like IL 17. This has a vital role in the destructive process of tissue and bone around the implant. Type 2 diabetes mellitus has the potential to increase these microbial colonies thus becoming a risk factor. To compare and correlate the association of peri-implant sulcular microflora and salivary IL-17 between healthy and controlled diabetic individuals. A total of 30 participants who have undergone dental implant placement with screw-retained prosthesis were included in the study. Participants were divided into two groups, healthy ( n = 15) and diabetic ( n = 15). The subgingival plaque was collected from peri-implant sites into TE buffer vials and the salivary sample was collected into Eppendorf tubes at the 7th, 14th, and 30th day. Microbiological counts of P. gingivalis, T. denticola, T .forsythia, and F. nucleatum were evaluated by polymerase chain reaction, and salivary interleukin (IL)-17 levels were obtained by enzyme-linked immunosorbent assay. The bacterial count was increased in both healthy and diabetic individuals but more prevalent in diabetes. The salivary IL-17 levels were increased in diabetic individuals which was statistically significant. Increased microbiota in both healthy and diabetic individuals along with increased IL-17 levels can lead to peri-implantitis which provides an insight into microbiological and immunological aspects in individuals with dental implants further acting as a predictor for peri-implantitis.