{"title":"supF 基因双链 DNA 长链上形成的超透明突变热点的解读","authors":"H. Grebneva","doi":"10.29039/rusjbpc.2023.0598","DOIUrl":null,"url":null,"abstract":"At present, the mechanism of formation of hot and cold spots of ultraviolet mutagenesis is not clear. I developed a polymerase-tautomeric model of the mechanism of formation of hot and cold spots of ultraviolet mutagenesis and showed that the probability of mutation formation depends on the processes of propagation of excitation energy along the DNA molecule. In my proposed polymerase-tautomeric model of ultraviolet mutagenesis, it was shown that mutations are formed opposite only those cis-syn cyclobutane pyrimidine dimers, one or both of which are in rare tautomeric forms. In the polymerase-tautomeric model of the mechanism of formation of hot and cold spots of ultraviolet mutagenesis, I have shown that the hot spots of ultraviolet mutagenesis are those cis-syn cyclobutane pyrimidine dimers to which the most excitation energy is transferred. In a number of works, I calculated the relative probabilities of mutations formed opposite the DNA bases that are part of the cis-syn cyclobutane pyrimidine dimers that appeared upon irradiation of double-stranded DNA of the supF gene. In this article, based on the results of previous calculations, I interpret experimental data in which hot spots of ultraviolet mutagenesis are DNA regions consisting of three or more pyrimidine DNA bases arranged in a row.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"102 15","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"INTERPRETATION OF HOT SPOTS OF ULTRAVOLETIC MUTAGENESIS FORMED ON A LAGGING STRAND OF DOUBLE-STRANDED DNA OF THE supF GENE\",\"authors\":\"H. Grebneva\",\"doi\":\"10.29039/rusjbpc.2023.0598\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"At present, the mechanism of formation of hot and cold spots of ultraviolet mutagenesis is not clear. I developed a polymerase-tautomeric model of the mechanism of formation of hot and cold spots of ultraviolet mutagenesis and showed that the probability of mutation formation depends on the processes of propagation of excitation energy along the DNA molecule. In my proposed polymerase-tautomeric model of ultraviolet mutagenesis, it was shown that mutations are formed opposite only those cis-syn cyclobutane pyrimidine dimers, one or both of which are in rare tautomeric forms. In the polymerase-tautomeric model of the mechanism of formation of hot and cold spots of ultraviolet mutagenesis, I have shown that the hot spots of ultraviolet mutagenesis are those cis-syn cyclobutane pyrimidine dimers to which the most excitation energy is transferred. In a number of works, I calculated the relative probabilities of mutations formed opposite the DNA bases that are part of the cis-syn cyclobutane pyrimidine dimers that appeared upon irradiation of double-stranded DNA of the supF gene. In this article, based on the results of previous calculations, I interpret experimental data in which hot spots of ultraviolet mutagenesis are DNA regions consisting of three or more pyrimidine DNA bases arranged in a row.\",\"PeriodicalId\":169374,\"journal\":{\"name\":\"Russian Journal of Biological Physics and Chemisrty\",\"volume\":\"102 15\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-05-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Russian Journal of Biological Physics and Chemisrty\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.29039/rusjbpc.2023.0598\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Russian Journal of Biological Physics and Chemisrty","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.29039/rusjbpc.2023.0598","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
目前,紫外线诱变冷热点的形成机制尚不清楚。我建立了紫外线诱变冷热点形成机制的聚合酶-同分异构体模型,并证明突变形成的概率取决于激发能量沿 DNA 分子传播的过程。在我提出的紫外线诱变的聚合酶-同分异构体模型中,只有那些顺式-辛式环丁烷嘧啶二聚体(其中一个或两个都是罕见的同分异构体)的对面才会形成突变。在紫外线诱变热点和冷点形成机制的聚合酶-同分异构体模型中,我已经证明紫外线诱变的热点是那些顺式-合成环丁烷嘧啶二聚体,激发能量传递到这些二聚体上的能量最多。在一些著作中,我计算了在辐照 supF 基因的双链 DNA 时,作为顺式-合成环丁烷嘧啶二聚体一部分的 DNA 碱基相对发生突变的相对概率。在这篇文章中,我根据以前的计算结果,解释了紫外线诱变热点是由三个或更多嘧啶 DNA 碱基排列成一行的 DNA 区域的实验数据。
INTERPRETATION OF HOT SPOTS OF ULTRAVOLETIC MUTAGENESIS FORMED ON A LAGGING STRAND OF DOUBLE-STRANDED DNA OF THE supF GENE
At present, the mechanism of formation of hot and cold spots of ultraviolet mutagenesis is not clear. I developed a polymerase-tautomeric model of the mechanism of formation of hot and cold spots of ultraviolet mutagenesis and showed that the probability of mutation formation depends on the processes of propagation of excitation energy along the DNA molecule. In my proposed polymerase-tautomeric model of ultraviolet mutagenesis, it was shown that mutations are formed opposite only those cis-syn cyclobutane pyrimidine dimers, one or both of which are in rare tautomeric forms. In the polymerase-tautomeric model of the mechanism of formation of hot and cold spots of ultraviolet mutagenesis, I have shown that the hot spots of ultraviolet mutagenesis are those cis-syn cyclobutane pyrimidine dimers to which the most excitation energy is transferred. In a number of works, I calculated the relative probabilities of mutations formed opposite the DNA bases that are part of the cis-syn cyclobutane pyrimidine dimers that appeared upon irradiation of double-stranded DNA of the supF gene. In this article, based on the results of previous calculations, I interpret experimental data in which hot spots of ultraviolet mutagenesis are DNA regions consisting of three or more pyrimidine DNA bases arranged in a row.