人沃顿果冻间充质干细胞(hWJ-MSCs)分泌物凝胶在紫外线诱导的小鼠模型中的潜在抗衰老活性。

IF 2.1 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Iranian Journal of Basic Medical Sciences Pub Date : 2024-01-01 DOI:10.22038/IJBMS.2024.70825.15385
Wahyu Widowati, Ahmad Faried, Achmad Adam, Deni Rahmat, Hanna Sari Widya Kusuma, Nindia Salsabila Mia Dewi, Marisca Evalina Gondokesumo, Rizal Rizal, Ita Margaretha Nainggolan, Massoud Vosough
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引用次数: 0

摘要

目的:皮肤老化是一个退化过程,可由紫外线照射诱发。紫外线辐射会产生活性氧化应激,导致皮肤过早老化。本研究的目的是在紫外线诱导的小鼠模型中研究人沃顿果冻间充质干细胞(hWJ-MSCs)分泌物凝胶(SC)的抗衰老潜力:从人沃顿果冻间充质干细胞(hWJ-MSCs)中提取分泌物并制成凝胶状。雄性小鼠接受紫外线照射,每天两次,每次 15 分钟,连续 14 天。将凝胶局部涂抹于小鼠背侧皮肤。泌素凝胶有两种处理方法:泌素 1 在紫外线照射后涂抹一次,泌素 2 在紫外线照射后每天涂抹两次。用 RT-PCR 法测定 TGF-β1、IL-10 和 IL-18 基因的表达。采用苏木精染色法观察皮肤组织的炎症和胶原蛋白密度。免疫组化法分析 P53、COL4A1、MMP-2 和 MMP-13 的蛋白表达。采用方差分析对数据进行统计分析,然后进行Tukey事后检验(PResults:紫外线诱导导致胶原蛋白流失、炎症加剧和衰老介质的高表达。SC增加了TGF-β1和IL-10的基因表达,降低了IL-18的基因表达。组织病理学测试表明,SG 增加了胶原蛋白密度,降低了炎症反应,修复了皮肤组织的细胞损伤。免疫组化检测显示,SC 降低了 MMP-2、MMP-13 和 P53 的表达,相反,增加了 COL4A1 的表达:结论:hWJ-间充质干细胞的分泌物凝胶具有抗衰老活性,有望预防和治疗皮肤衰老。
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Potential antiaging activity of secretome gel of human Wharton's jelly mesenchymal stem cells (hWJ-MSCs) in UV-induced mice models.

Objectives: Skin aging is a degenerative process that can be induced by UV irradiation. UV radiation can produce reactive oxidate stress which causes premature aging. This study aims to examine the antiaging potential of secretome gel (SC) from human Wharton Jelly Mesenchymal Stem Cells (hWJ-MSCs) in a UVB-induced mice model.

Materials and methods: The secretome was obtained from hWJ-MSCs and made in gel form. Male mice were radiated by UVB for 15 min twice daily for 14 days. The gel was topically applied to the mice's dorsal skin. Two treatments of secretome gel: secretome 1 is applied once and secretome 2 is applied twice daily after UVB radiation. TGF-β1, IL-10, and IL-18 gene expression was determined using RT-PCR. Hematoxylin Eosin staining was used to observe the inflammation and collagen density of skin tissue. An immunohistochemistry assay was used to analyze the protein expression of P53, COL4A1, MMP-2, and MMP-13. The data were statistically analyzed using the ANOVA test followed by the Tukey post hoc test (P<0.05).

Results: UVB induction caused loss of collagen, increasing inflammation and high expression of aging mediators. SC increased the gene expression of TGF-β1 and IL-10 and decreased IL-18 gene expression. Histopathological tests showed that SG increased collagen density, lowered inflammation, and repaired cell damage in skin tissue. Immunohistochemistry test showed that SC decreased MMP-2, MMP-13, and P53 expression, in contrast, increased COL4A1.

Conclusion: The secretome gel of hWJ-MSCs showed antiaging activities with potential for preventing and curing skin aging.

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来源期刊
Iranian Journal of Basic Medical Sciences
Iranian Journal of Basic Medical Sciences MEDICINE, RESEARCH & EXPERIMENTAL-PHARMACOLOGY & PHARMACY
CiteScore
4.00
自引率
4.50%
发文量
142
审稿时长
6-12 weeks
期刊介绍: The Iranian Journal of Basic Medical Sciences (IJBMS) is a peer-reviewed, monthly publication by Mashhad University of Medical Sciences (MUMS), Mashhad, Iran . The Journal of "IJBMS” is a modern forum for scientific communication. Data and information, useful to investigators in any discipline in basic medical sciences mainly including Anatomical Sciences, Biochemistry, Genetics, Immunology, Microbiology, Pathology, Pharmacology, Pharmaceutical Sciences, and Physiology, will be published after they have been peer reviewed. This will also include reviews and multidisciplinary research.
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