激素替代疗法可调节刺激性挑战对绝经后妇女皮肤屏障和骨髓驻留免疫细胞的影响。

IF 11 1区 医学 Q1 DERMATOLOGY British Journal of Dermatology Pub Date : 2024-10-17 DOI:10.1093/bjd/ljae226
Orsolya Kiss, Rajia Bahri, Rachel E B Watson, Chidera Chike, Abigail K Langton, Victoria L Newton, Mike Bell, Christopher E M Griffiths, Silvia Bulfone-Paus, Suzanne M Pilkington
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引用次数: 0

摘要

背景:绝经过渡期的性激素变化会导致皮肤健康状况下降。然而,更年期及其激素替代疗法(HRT)如何影响皮肤屏障和免疫系统尚不清楚。因此,我们研究了绝经和激素替代疗法如何影响绝经后妇女在接受刺激物挑战后的皮肤屏障和免疫细胞组成:研究招募了两组绝经后妇女,一组未接受治疗(HRT-;n = 10;平均年龄 56.5 岁 [范围 48-63 岁]),另一组接受 HRT(n = 8;平均年龄 54 岁 [范围 48-63 岁])。在闭塞的臀部皮肤上局部涂抹 1.25% 的十二烷基硫酸钠(SLS),持续 48 小时,诱发皮肤过敏。24 小时后进行临床评估,然后对受 SLS 刺激和未受刺激的皮肤进行活组织检查,用免疫荧光法分析皮肤屏障蛋白和免疫细胞分布:在临床上,服用或未服用 HRT(包括皮肤发红和血流量增加)的人的皮肤刺激反应没有明显差异。在应对 SLS 挑战时,经表皮失水率(pConclusion)显著增加:接受 HRT 的人对 SLS 挑战的皮肤屏障反应增强,丝胶蛋白变厚,角蛋白-10 阳性表皮细胞层增加。接受 SLS 挑战后,HRT 使用者的真皮层中 LCs、炎性 DCs 和巨噬细胞的数量增加。这可能会使皮肤更容易发炎,也更有能力消除炎症,同时促进皮肤修复。
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The impact of irritant challenge on the skin barrier and myeloid-resident immune cells in women who are postmenopausal is modulated by hormone replacement therapy.

Background: Sex hormone changes during menopausal transition contribute to declining skin health. However, how menopause and its treatment by hormone replacement therapy (HRT) impact the skin barrier and immune system is unclear.

Objectives: To examine how menopause and HRT affect the skin barrier and immune cell composition in postmenopausal women following irritant challenge.

Methods: Two cohorts of postmenopausal women were recruited to the study. The first cohort consisted of 10 untreated women [HRT-; mean (SEM) age 56.5 (1.6) years (range 48-63)] and the second was composed of 8 women receiving HRT [HRT+; mean (SEM) age 54.0 (2.1) years (range 48-63)]. Skin irritation was induced by applying topical sodium lauryl sulfate (SLS) 1.25% to occluded buttock skin for 48 h. Clinical assessment was conducted after 24 h, followed by biopsy of both SLS-challenged and unchallenged skin for analysis of skin barrier proteins and immune cell distribution using immunofluorescence.

Results: Clinically, there were no significant differences in skin irritant responses between those taking or not taking HRT (including increased skin redness and blood flow). In response to SLS challenge a significant increase in transepidermal water loss (P < 0.05), filaggrin deposition and cytokeratin 10 (K10)+ cell layers (P < 0.01) was observed in individuals receiving HRT compared with the HRT- group. Following SLS challenge in individuals taking HRT, a significant (P < 0.01) reduction in CD207+ cells in the epidermis was observed, accompanied by an increase of CD207+ cells in the dermis, indicative of migrating Langerhans cells (LCs). Significantly fewer migrating LCs were found in those who were not receiving HRT (P < 0.01). Furthermore, the numbers of dermal dendritic cells (DCs), macrophages, and CD11c+CD206- and CD68+CD206- subsets were found to be significantly (P < 0.05) higher in those taking HRT following SLS challenge.

Conclusions: Individuals receiving HRT displayed enhanced skin barrier response to SLS challenge with thicker filaggrin and increased K10+ epidermal cell layers. Following challenge, HRT users exhibited elevated LC, inflammatory DC and macrophage counts in the dermis. These may render skin both more prone to inflammation and more capable of resolving it, while also promoting skin repair.

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来源期刊
British Journal of Dermatology
British Journal of Dermatology 医学-皮肤病学
CiteScore
16.30
自引率
3.90%
发文量
1062
审稿时长
2-4 weeks
期刊介绍: The British Journal of Dermatology (BJD) is committed to publishing the highest quality dermatological research. Through its publications, the journal seeks to advance the understanding, management, and treatment of skin diseases, ultimately aiming to improve patient outcomes.
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