Amy M. Kahler , Jessica Hofstetter , Michael Arrowood , Anna Peterson , David Jacobson , Joel Barratt , Andre Luiz Biscaia Ribeiro da Silva , Camila Rodrigues , Mia C. Mattioli
{"title":"美国东南部种植环境中卡耶坦环孢子虫的来源和流行情况。","authors":"Amy M. Kahler , Jessica Hofstetter , Michael Arrowood , Anna Peterson , David Jacobson , Joel Barratt , Andre Luiz Biscaia Ribeiro da Silva , Camila Rodrigues , Mia C. Mattioli","doi":"10.1016/j.jfp.2024.100309","DOIUrl":null,"url":null,"abstract":"<div><p>Recent cyclosporiasis outbreaks associated with fresh produce grown in the United States highlight the need to better understand <em>Cyclospora cayetanensis</em> prevalence in U.S. agricultural environments. In this study, <em>C. cayetanensis</em> occurrence was assessed in municipal wastewater sludge, on-farm portable toilets, irrigation pond water, and spent packing house dump tank water in a Southeastern Georgia growing region over two years. Detection of the <em>C. cayetanensis</em> 18S rRNA qPCR gene target in pond samples was 0%, 28%, and 42% (<em>N</em> = 217) depending on the detection definition used, and ≤1% in dump tank samples (<em>N</em> = 46). However, no qPCR detections were confirmed by sequencing, suggesting false detection occurred due to cross-reactions. <em>C. cayetanensis</em> qPCR detections were confirmed in 9% of wastewater sludge samples (<em>N</em> = 76). The human-specific fecal markers HF183 and crAssphage were detected in 33% and 6% of pond samples, respectively, and 4% and 0% of dump tank samples, respectively. Despite community <em>Cyclospora</em> shedding and evidence of human fecal contamination in irrigation water, there was no correlation between <em>C. cayetanensis</em> and HF183 qPCR detections, further supporting that 18S gene target qPCR amplifications were due to cross−reactions. When evaluating <em>C. cayetanensis</em> qPCR environmental detection data, the impact of assay specificity and detection criteria should be considered. Moreover, additional sequence-based testing may be needed to appropriately interpret <em>Cyclospora</em> qPCR environmental data.</p></div>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0362028X24000930/pdfft?md5=e4aa66024a31dbe283a9daa18395bc5a&pid=1-s2.0-S0362028X24000930-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Sources and Prevalence of Cyclospora cayetanensis in Southeastern U.S. Growing Environments\",\"authors\":\"Amy M. Kahler , Jessica Hofstetter , Michael Arrowood , Anna Peterson , David Jacobson , Joel Barratt , Andre Luiz Biscaia Ribeiro da Silva , Camila Rodrigues , Mia C. Mattioli\",\"doi\":\"10.1016/j.jfp.2024.100309\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Recent cyclosporiasis outbreaks associated with fresh produce grown in the United States highlight the need to better understand <em>Cyclospora cayetanensis</em> prevalence in U.S. agricultural environments. In this study, <em>C. cayetanensis</em> occurrence was assessed in municipal wastewater sludge, on-farm portable toilets, irrigation pond water, and spent packing house dump tank water in a Southeastern Georgia growing region over two years. Detection of the <em>C. cayetanensis</em> 18S rRNA qPCR gene target in pond samples was 0%, 28%, and 42% (<em>N</em> = 217) depending on the detection definition used, and ≤1% in dump tank samples (<em>N</em> = 46). However, no qPCR detections were confirmed by sequencing, suggesting false detection occurred due to cross-reactions. <em>C. cayetanensis</em> qPCR detections were confirmed in 9% of wastewater sludge samples (<em>N</em> = 76). The human-specific fecal markers HF183 and crAssphage were detected in 33% and 6% of pond samples, respectively, and 4% and 0% of dump tank samples, respectively. Despite community <em>Cyclospora</em> shedding and evidence of human fecal contamination in irrigation water, there was no correlation between <em>C. cayetanensis</em> and HF183 qPCR detections, further supporting that 18S gene target qPCR amplifications were due to cross−reactions. When evaluating <em>C. cayetanensis</em> qPCR environmental detection data, the impact of assay specificity and detection criteria should be considered. Moreover, additional sequence-based testing may be needed to appropriately interpret <em>Cyclospora</em> qPCR environmental data.</p></div>\",\"PeriodicalId\":15903,\"journal\":{\"name\":\"Journal of food protection\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2024-05-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S0362028X24000930/pdfft?md5=e4aa66024a31dbe283a9daa18395bc5a&pid=1-s2.0-S0362028X24000930-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of food protection\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0362028X24000930\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of food protection","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0362028X24000930","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Sources and Prevalence of Cyclospora cayetanensis in Southeastern U.S. Growing Environments
Recent cyclosporiasis outbreaks associated with fresh produce grown in the United States highlight the need to better understand Cyclospora cayetanensis prevalence in U.S. agricultural environments. In this study, C. cayetanensis occurrence was assessed in municipal wastewater sludge, on-farm portable toilets, irrigation pond water, and spent packing house dump tank water in a Southeastern Georgia growing region over two years. Detection of the C. cayetanensis 18S rRNA qPCR gene target in pond samples was 0%, 28%, and 42% (N = 217) depending on the detection definition used, and ≤1% in dump tank samples (N = 46). However, no qPCR detections were confirmed by sequencing, suggesting false detection occurred due to cross-reactions. C. cayetanensis qPCR detections were confirmed in 9% of wastewater sludge samples (N = 76). The human-specific fecal markers HF183 and crAssphage were detected in 33% and 6% of pond samples, respectively, and 4% and 0% of dump tank samples, respectively. Despite community Cyclospora shedding and evidence of human fecal contamination in irrigation water, there was no correlation between C. cayetanensis and HF183 qPCR detections, further supporting that 18S gene target qPCR amplifications were due to cross−reactions. When evaluating C. cayetanensis qPCR environmental detection data, the impact of assay specificity and detection criteria should be considered. Moreover, additional sequence-based testing may be needed to appropriately interpret Cyclospora qPCR environmental data.
期刊介绍:
The Journal of Food Protection® (JFP) is an international, monthly scientific journal in the English language published by the International Association for Food Protection (IAFP). JFP publishes research and review articles on all aspects of food protection and safety. Major emphases of JFP are placed on studies dealing with:
Tracking, detecting (including traditional, molecular, and real-time), inactivating, and controlling food-related hazards, including microorganisms (including antibiotic resistance), microbial (mycotoxins, seafood toxins) and non-microbial toxins (heavy metals, pesticides, veterinary drug residues, migrants from food packaging, and processing contaminants), allergens and pests (insects, rodents) in human food, pet food and animal feed throughout the food chain;
Microbiological food quality and traditional/novel methods to assay microbiological food quality;
Prevention of food-related hazards and food spoilage through food preservatives and thermal/non-thermal processes, including process validation;
Food fermentations and food-related probiotics;
Safe food handling practices during pre-harvest, harvest, post-harvest, distribution and consumption, including food safety education for retailers, foodservice, and consumers;
Risk assessments for food-related hazards;
Economic impact of food-related hazards, foodborne illness, food loss, food spoilage, and adulterated foods;
Food fraud, food authentication, food defense, and foodborne disease outbreak investigations.