{"title":"拓宽靶向空间：PAM 灵活 Cas 蛋白的工程设计与发现。","authors":"Zhenkun Cai, Weiyu Xie, Zehua Bao","doi":"10.1016/j.tim.2024.05.004","DOIUrl":null,"url":null,"abstract":"<p><p>The application of CRISPR-Cas systems has been hindered by their requirement for long protospacer-adjacent motifs (PAMs). Recent engineering and discovery of PAM-flexible Cas proteins have substantially broadened the targetable DNA sequence space, thereby facilitating genome editing and improving derivative technologies such as gene regulation, seamless cloning, and large-scale genetic screens.</p>","PeriodicalId":23275,"journal":{"name":"Trends in Microbiology","volume":null,"pages":null},"PeriodicalIF":14.0000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Broadening the targetable space: engineering and discovery of PAM-flexible Cas proteins.\",\"authors\":\"Zhenkun Cai, Weiyu Xie, Zehua Bao\",\"doi\":\"10.1016/j.tim.2024.05.004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The application of CRISPR-Cas systems has been hindered by their requirement for long protospacer-adjacent motifs (PAMs). Recent engineering and discovery of PAM-flexible Cas proteins have substantially broadened the targetable DNA sequence space, thereby facilitating genome editing and improving derivative technologies such as gene regulation, seamless cloning, and large-scale genetic screens.</p>\",\"PeriodicalId\":23275,\"journal\":{\"name\":\"Trends in Microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":14.0000,\"publicationDate\":\"2024-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Trends in Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1016/j.tim.2024.05.004\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/5/29 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Trends in Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1016/j.tim.2024.05.004","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/5/29 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
CRISPR-Cas系统的应用一直受制于对长原位相邻基序（PAM）的要求。最近，PAM 灵活 Cas 蛋白的工程设计和发现大大拓宽了可靶向的 DNA 序列空间，从而促进了基因组编辑，并改善了基因调控、无缝克隆和大规模基因筛选等衍生技术。
Broadening the targetable space: engineering and discovery of PAM-flexible Cas proteins.
The application of CRISPR-Cas systems has been hindered by their requirement for long protospacer-adjacent motifs (PAMs). Recent engineering and discovery of PAM-flexible Cas proteins have substantially broadened the targetable DNA sequence space, thereby facilitating genome editing and improving derivative technologies such as gene regulation, seamless cloning, and large-scale genetic screens.
期刊介绍：
Trends in Microbiology serves as a comprehensive, multidisciplinary forum for discussing various aspects of microbiology, spanning cell biology, immunology, genetics, evolution, virology, bacteriology, protozoology, and mycology. In the rapidly evolving field of microbiology, technological advancements, especially in genome sequencing, impact prokaryote biology from pathogens to extremophiles, influencing developments in drugs, vaccines, and industrial enzyme research.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
