间歇压缩力可调节人牙周韧带细胞中基质金属蛋白酶和组织金属蛋白酶抑制剂的表达。

IF 2.2 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Archives of oral biology Pub Date : 2024-05-27 DOI:10.1016/j.archoralbio.2024.106011
Novena Dameria Pakpahan , Maythwe Kyawsoewin , Jeeranan Manokawinchoke , Worachat Namangkalakul , Chutimon Termkwancharoen , Hiroshi Egusa , Phoonsuk Limraksasin , Thanaphum Osathanon
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引用次数: 0

摘要

研究目的设计:将人牙周韧带细胞(hPDLCs)置于1.5 g/cm2的间歇压缩力(ICF)作用下并负载24 h,使用RT-PCR和ELISA分析评估几种MMPs和TIMPs的mRNA和蛋白表达。结果:mRNA 和蛋白质分析表明,ICF 显著诱导了 TIMP1 和 TIMP3 的表达,但降低了 MMP1 的表达。用 TGF-β 抑制剂孵育并应用于 ICF 显示 TIMP3 的表达下调,但 MMP1 的表达未受影响:结论:ICF 可能通过调节 MMP1 和 TIMPs 的表达来影响 hPDLC 的 ECM 稳态。此外,TGF-β1 还能调节 TIMP3 的表达。这些研究结果表明,ICF 可减少 ECM 的降解,因此可能对维持 PDL 的稳态至关重要。
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Intermittent compressive force regulates matrix metalloproteinases and tissue inhibitors of metalloproteinases expression in human periodontal ligament cells

Objective

This study aims to evaluate the effects of intermittent compressive force (ICF) on the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) by human periodontal ligament cells (hPDLCs).

Design

hPDLCs were subjected to ICF with a magnitude of 1.5 g/cm2 and loaded for 24 h. mRNA and protein expression of several MMPs and TIMPs were assessed using RT-PCR and ELISA analyses. An inhibitor of TGF-β (SB431542) was used to assess a possible role of TGF-β in the expression of MMPs and TIMPs under ICF.

Results

mRNA and protein analyses showed that ICF significantly induced expression of TIMP1 and TIMP3, but decreased expression of MMP1. Incubation with the TGF-β inhibitor and applied to ICF showed a downregulation of TIMP3, but expression of MMP1 was not affected.

Conclusion

ICF is likely to affect ECM homeostasis by hPDLCs by regulating the expression of MMP1 and TIMPs. Moreover, TGF-β1 regulated expression of TIMP3. These findings suggest ICF may decrease the degradation of ECM and may thus be essential for maintaining PDL homeostasis.

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来源期刊
Archives of oral biology
Archives of oral biology 医学-牙科与口腔外科
CiteScore
5.10
自引率
3.30%
发文量
177
审稿时长
26 days
期刊介绍: Archives of Oral Biology is an international journal which aims to publish papers of the highest scientific quality in the oral and craniofacial sciences. The journal is particularly interested in research which advances knowledge in the mechanisms of craniofacial development and disease, including: Cell and molecular biology Molecular genetics Immunology Pathogenesis Cellular microbiology Embryology Syndromology Forensic dentistry
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