揭示植物特殊代谢途径的化学工具

IF 8.3 2区 生物学 Q1 PLANT SCIENCES Current opinion in plant biology Pub Date : 2024-05-30 DOI:10.1016/j.pbi.2024.102554
Benedikt Seligmann , Shenyu Liu , Jakob Franke
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引用次数: 0

摘要

阐明植物中特殊代谢物的生化途径是实现或改进其可持续生物技术生产的关键。化学工具可以极大地促进生物合成基因和酶的发现。在此,我们总结了跨学科方法,其中化学和化学生物学的方法有助于克服途径阐明的关键挑战。根据最近的实例,我们介绍了最先进的同位素标记实验如何指导候选生物合成基因的选择,基于亲和力的探针如何使新型酶的鉴定成为可能,半合成如何提高难以捉摸的途径中间体的可用性,以及仿生反应如何使人们更好地了解固有的化学反应性。我们预计,此类化学方法的更广泛应用将加快植物途径阐明的步伐。
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Chemical tools for unpicking plant specialised metabolic pathways

Elucidating the biochemical pathways of specialised metabolites in plants is key to enable or improve their sustainable biotechnological production. Chemical tools can greatly facilitate the discovery of biosynthetic genes and enzymes. Here, we summarise transdisciplinary approaches where methods from chemistry and chemical biology helped to overcome key challenges of pathway elucidation. Based on recent examples, we describe how state-of-the-art isotope labelling experiments can guide the selection of biosynthetic gene candidates, how affinity-based probes enable the identification of novel enzymes, how semisynthesis can improve the availability of elusive pathway intermediates, and how biomimetic reactions provide a better understanding of inherent chemical reactivity. We anticipate that a wider application of such chemical methods will accelerate the pace of pathway elucidation in plants.

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来源期刊
Current opinion in plant biology
Current opinion in plant biology 生物-植物科学
CiteScore
16.30
自引率
3.20%
发文量
131
审稿时长
6-12 weeks
期刊介绍: Current Opinion in Plant Biology builds on Elsevier's reputation for excellence in scientific publishing and long-standing commitment to communicating high quality reproducible research. It is part of the Current Opinion and Research (CO+RE) suite of journals. All CO+RE journals leverage the Current Opinion legacy - of editorial excellence, high-impact, and global reach - to ensure they are a widely read resource that is integral to scientists' workflow.
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