运动诱导的肌肉激素 "鸢尾素 "可控制牛促性腺激素分泌黄体生成素和卵泡刺激素。

IF 2.2 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Animal Reproduction Science Pub Date : 2024-05-29 DOI:10.1016/j.anireprosci.2024.107516
Yvan Bienvenu Niyonzima, Denis Karani Wanjiru, Hiroya Kadokawa
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引用次数: 0

摘要

鸢尾素是肌肉在运动时分泌的一种激素。鸢尾素受体(IrisinR)是整合素αV(ITGAV)和整合素β5(ITGB5)亚基的异二聚体。由于鸢尾素可能介导运动对动物繁殖的一些有益影响,我们对牛性腺表达鸢尾素受体(IrisinR)和鸢尾素刺激性腺分泌黄体生成素(LH)和促卵泡激素(FSH)的假设进行了测试。逆转录聚合酶链反应用于检测青春期后母牛垂体前叶(AP)和小鼠促性腺激素细胞系 "LβT2 "中 ITGAV 和 ITGB5 的 mRNA 表达。采用 Western 印迹法检测牛垂体前叶的蛋白表达。免疫荧光显微镜使用相同的抗体在大多数性腺细胞的质膜上观察到了IrisinR。我们从健康的青春期后母牛身上制备了AP细胞,培养了3.5天,然后在不处理或促性腺激素释放激素(GnRH)刺激前用浓度递增(0、0.01、0.1、1或10 nM)的鸢尾素处理它们5分钟。2 小时后,收获培养基进行 LH 和 FSH 检测。鸢尾素(0.1-10 nM)可刺激基础 LH 和 FSH 分泌,细胞外信号调节激酶或 SMAD 通路抑制剂可抑制这些刺激作用。在有 GnRH 的情况下,0.01-1 nM 的鸢尾素能刺激 LH 和 FSH 的分泌。然而,较高剂量的鸢尾素(10 nM)会抑制 GnRH 诱导的 LH 和 FSH 水平。总之,牛性腺细胞表达鸢尾素R,鸢尾素控制牛性腺细胞的LH和FSH分泌。
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Exercise-induced muscle hormone “irisin” controls luteinizing hormone and follicle-stimulating hormone secretion by bovine gonadotrophs

Irisin is a hormone secreted by muscle in response to exercise. The irisin receptor (IrisinR) is a heterodimer of integrin alpha V (ITGAV) and integrin beta 5 (ITGB5) subunits. Since irisin may mediate some beneficial effects of exercise on animal reproduction, we tested the hypothesis that bovine gonadotrophs express IrisinR and irisin stimulates luteinizing hormone (LH) and follicle stimulating hormone (FSH) secretion by gonadotrophs. Reverse transcription polymerase chain reaction was used to detect the mRNA expression of both ITGAV and ITGB5 in the anterior pituitary glands (APs) of post pubertal heifers and mouse gonadotroph cell line “LβT2.” Western blotting was used to detect protein expression in bovine APs. Immunofluorescence microscopy, utilizing the same antibody, visualized IrisinR on the plasma membrane of majority of gonadotrophs. We prepared AP cells from healthy postpubertal heifers, cultured them for 3.5 d, and treated them with increasing concentrations (0, 0.01, 0.1, 1, or 10 nM) of irisin for 5 min before either no treatment or gonadotropin-releasing hormone (GnRH) stimulation. After 2 h, media were harvested for LH and FSH assays. Irisin (0.1–10 nM) stimulated basal LH and FSH secretion, and these stimulatory effects were inhibited by the extracellular signal-regulated kinase or SMAD pathway inhibitors. In the presence of GnRH, irisin at 0.01–1 nM stimulated LH and FSH secretion. A higher dose of irisin (10 nM), however, suppressed the GnRH-induced LH and FSH levels. In conclusion, bovine gonadotrophs expressed IrisinR, and irisin controlled LH and FSH secretion from bovine gonadotrophs.

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来源期刊
Animal Reproduction Science
Animal Reproduction Science 农林科学-奶制品与动物科学
CiteScore
4.50
自引率
9.10%
发文量
136
审稿时长
54 days
期刊介绍: Animal Reproduction Science publishes results from studies relating to reproduction and fertility in animals. This includes both fundamental research and applied studies, including management practices that increase our understanding of the biology and manipulation of reproduction. Manuscripts should go into depth in the mechanisms involved in the research reported, rather than a give a mere description of findings. The focus is on animals that are useful to humans including food- and fibre-producing; companion/recreational; captive; and endangered species including zoo animals, but excluding laboratory animals unless the results of the study provide new information that impacts the basic understanding of the biology or manipulation of reproduction. The journal''s scope includes the study of reproductive physiology and endocrinology, reproductive cycles, natural and artificial control of reproduction, preservation and use of gametes and embryos, pregnancy and parturition, infertility and sterility, diagnostic and therapeutic techniques. The Editorial Board of Animal Reproduction Science has decided not to publish papers in which there is an exclusive examination of the in vitro development of oocytes and embryos; however, there will be consideration of papers that include in vitro studies where the source of the oocytes and/or development of the embryos beyond the blastocyst stage is part of the experimental design.
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