本地嗜卤细菌 Halomonas elongata SEK2 的亚砷酸盐耐受性和去除潜力。

IF 4.1 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Biometals Pub Date : 2024-06-01 DOI:10.1007/s10534-024-00612-2
Nazanin Tavoosi, Abbas Akhavan Sepahi, Vahid Kiarostami, Mohammad Ali Amoozegar
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引用次数: 0

摘要

从伊朗卢特沙漠马利克-穆罕默德洞的高盐度土壤中分离出的本地嗜卤抗砷细菌 Halomonas elongata 菌株 SEK2 在 SW-10 琼脂培养基中可耐受分别高达 800 和 40 mM 的高浓度砷酸盐(As5+)和亚砷酸盐(As3+)。分离出的菌株还能耐受相当高浓度的其他有毒重金属和氧阴离子,包括镉(Cd2+)、铬酸盐(Cr6+)、铅(Pb2+)和亚硒酸盐(Se4+),在培养基盐度较高(总盐浓度为 10%(w/v))的情况下,分离出的 SEK2 的耐受潜力是前所未有的。通过二乙基二硫代氨基甲酸银(SDDC)法检测了分离株 SEK2 的生物去除潜力,结果表明,培养 48 小时后,分离株 SEK2 可从含亚砷酸盐的生长培养基中去除 60% 的亚砷酸盐,而不会将其转化为砷酸盐。傅立叶变换红外光谱(FTIR)、扫描电子显微镜(SEM)和能量色散 X 射线(EDX)分析证实了嗜卤细菌对亚砷酸盐的吸附或吸收。此外,透射电子显微镜(TEM)分析表明,在亚砷酸盐存在的情况下,细菌细胞的超结构发生了改变,这可能是由于亚砷酸盐在细胞内积累所致。基因组测序分析表明,该细菌菌株的基因组中存在亚砷酸盐抗性基因以及其他重金属/氧化物抗性基因。因此,Halomonas elongata 菌株 SEK2 被首次鉴定为耐亚砷酸盐的嗜卤细菌,可用于亚砷酸盐污染的盐碱环境的生物修复。
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Arsenite tolerance and removal potential of the indigenous halophilic bacterium, Halomonas elongata SEK2.

The indigenous halophilic arsenite-resistant bacterium Halomonas elongata strain SEK2 isolated from the high saline soil of Malek Mohammad hole, Lut Desert, Iran, could tolerate high concentrations of arsenate (As5+) and arsenite (As3+) up to 800 and 40 mM in the SW-10 agar medium, respectively. The isolated strain was able to tolerate considerable concentrations of other toxic heavy metals and oxyanions, including Cadmium (Cd2+), Chromate (Cr6+), lead (Pb2+), and selenite (Se4+), regarding the high salinity of the culture media (with a total salt concentration of 10% (w/v)), the tolerance potential of the isolate SEK2 was unprecedented. The bioremoval potential of the isolate SEK2 was examined through the Silver diethyldithiocarbamate (SDDC) method and demonstrated that the strain SEK2 could remove 60% of arsenite from arsenite-containing growth medium after 48 h of incubation without converting it to arsenate. The arsenite adsorption or uptake by the halophilic bacterium was investigated and substantiated through Fourier-transform infrared spectroscopy (FTIR), Scanning Electron Microscope (SEM), and Energy Dispersive X-ray (EDX) analyses. Furthermore, Transmission electron microscope (TEM) analysis revealed ultra-structural alterations in the presence of arsenite that could be attributed to intracellular accumulation of arsenite by the bacterial cell. Genome sequencing analysis revealed the presence of arsenite resistance as well as other heavy metals/oxyanion resistance genes in the genome of this bacterial strain. Therefore, Halomonas elongata strain SEK2 was identified as an arsenite-resistant halophilic bacterium for the first time that could be used for arsenite bioremediation in saline arsenite-polluted environments.

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来源期刊
Biometals
Biometals 生物-生化与分子生物学
CiteScore
5.90
自引率
8.60%
发文量
111
审稿时长
3 months
期刊介绍: BioMetals is the only established journal to feature the important role of metal ions in chemistry, biology, biochemistry, environmental science, and medicine. BioMetals is an international, multidisciplinary journal singularly devoted to the rapid publication of the fundamental advances of both basic and applied research in this field. BioMetals offers a forum for innovative research and clinical results on the structure and function of: - metal ions - metal chelates, - siderophores, - metal-containing proteins - biominerals in all biosystems. - BioMetals rapidly publishes original articles and reviews. BioMetals is a journal for metals researchers who practice in medicine, biochemistry, pharmacology, toxicology, microbiology, cell biology, chemistry, and plant physiology who are based academic, industrial and government laboratories.
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