Lignocellulolytic degradation using secondary metabolites of Trichoderma reesei UMK04

Aims: Fungi species received much attention due to their numerical ability to manufacture various enzymes that can be used to break down cellulose, starch and lipids. However, the conventional way of mycelial incorporation in lignocellulolytic agriculture materials shows less quantity for the degradation of organic compounds. Therefore, fungal metabolites extract received much attention for large amounts of degradation. On the other hand, the effectiveness of fungal metabolite extracts depends on the solvent reaction process. Thus, this study compared degrading organic compounds such as lignin and cellulose in a plate assay experiment using Trichoderma reesei UMK04 secondary metabolites extract using ethyl acetate and hexene solvents. Methodology and results: The T. reesei UMK04 was cultured in potato dextrose broth (PDB) media. Secondary metabolites from the broth culture of T. reseei were extracted using two different solvents of ethyl acetate and hexene separately. The degradation of organic compounds was determined in a plate assay experiment using Jensen media and Tannic acid media. Metabolite extract of T. reesei was used in concentrations of 5 mg/mL, 10 mg/mL, 15 mg/mL and 20 mg/mL. The hollow zone method was used to determine the degradation range. The ethyl acetate solvent extract showed the highest cellulose degradation-like pigmentation formation, which appeared at 6.6 cm on the plate, compared to lignin, which was 3.6 cm. Conclusion, significance and impact of study: The secondary metabolites of T. reesei using ethyl acetate extracts showed higher cellulose degradation, which showed that solvents play the role of more effective compound extraction.