实验诱发肝脓肿的母牛回肠上皮组织转录本图谱*†.

IF 1.4 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Applied Animal Science Pub Date : 2024-06-01 DOI:10.15232/aas.2023-02503
Amanda K. Lindholm-Perry , Brittney N. Keel , Kristin E. Hales , James E. Wells , Larry A. Kuehn , John W. Keele , Matthew S. Crouse , Dan J. Nonneman , T.G. Nagaraja , Ty E. Lawrence , Raghavendra G. Amachawadi , Jeff A. Carroll , Nicole C. Burdick Sanchez , Paul R. Broadway
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引用次数: 0

摘要

目的 牛肝脓肿对整个肉牛业的生产造成负面影响和经济损失。最近,人们开发了一种诱发肝脓肿的营养模型,该模型将为评估肝脓肿发生的潜在机制提供一种方法。本研究的目的是评估使用新模型的小牛回肠组织的基因表达。在该模型中,给小牛喂食诱发瘤胃酸中毒的饲料,然后在瘤胃内接种通常与肝脓肿有关的细菌。接种后第21天,犊牛被安乐死。13 头小牛中有 7 头出现肝脓肿。收集回肠组织并分离总 RNA 进行 RNA 测序。结果与讨论与未患肝脓肿的犊牛相比,患肝脓肿的犊牛共有 26 个基因有不同程度的表达。两个基因(COX2、COX3)是电子链转移途径中细胞色素 c 氧化酶的组成成分,这两个基因在肝脓肿动物中的表达量都有所下降。还发现了其他参与细胞重组、化合物跨细胞膜运输、保护 RNA 和保护蛋白质产物的基因。这些功能表明,回肠上皮细胞的变化与肝脓肿的发生是相对应的。 意义和应用我们发现,在使用酸中毒诱导饮食和腔内接种细菌相结合的方法治疗肝脓肿的小牛回肠组织中,有七种基因的表达量不同。我们的研究结果表明,回肠在肝脓肿的形成过程中起着一定的作用,今后有必要进行研究以验证回肠粘膜屏障是否参与了肝脓肿的形成。
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Ileal epithelial tissue transcript profiles of steers with experimentally induced liver abscesses*†

Objective

Liver abscesses in cattle negatively affect production and cause economic losses across the beef cat- tle industry. Recently, a nutritional model has been devel- oped to induce liver abscesses that will provide a method to evaluate the underlying mechanisms driving their de- velopment. The objective of this study was to evaluate gene expression of ileal tissue from calves challenged using the new model.

Materials and Methods

In the model, calves were fed a diet to induce ruminal acidosis and were then in- traruminally inoculated with bacteria typically associated with liver abscesses. On d 21, following inoculation, calves were euthanized. Seven of the 13 calves had liver abscess- es. Ileal tissues were collected and total RNA was isolated for RNA-sequencing.

Results and Discussion

A total of 26 genes were dif- ferentially expressed in the calves with liver abscesses com- pared with those without. Two genes (COX2, COX3) are components of the cytochrome c oxidase enzyme in the electron chain transport pathway, and both were downreg- ulated among the animals with liver abscesses. Other genes involved in cellular restructuring, transport of compounds across cell membranes, protection of RNA, and protection of protein products were also identified. These functions il- lustrate that there are changes in the ileal epithelium that correspond to the development of liver abscesses.

Implications and Applications

We identified sev- eral genes that were differentially expressed in the ileal tissue of calves that developed liver abscesses using the combination of an acidosis-inducing diet and intraruminal inoculation of bacteria. Our results suggest that the ileum has a role in liver abscess development, and future studies to validate the involvement of the mucosal barrier of the ileum are warranted.

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来源期刊
Applied Animal Science
Applied Animal Science AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
3.00
自引率
6.70%
发文量
68
期刊最新文献
Table of Contents Editorial Board Call for Submissions Fabrication yields and allometric growth coefficients of carcass components of serially slaughtered implanted or non-implanted beef steers Comparison of a single extended-release implant and a re-implant strategy on performance and carcass characteristics of beef finishing heifers
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