应用MicroRNA-124载体脂质体纳米粒子通过靶向BECN1抑制胰腺癌细胞进展和抑制自噬

IF 2.9 4区 医学 Q1 Medicine Journal of biomedical nanotechnology Pub Date : 2024-06-01 DOI:10.1166/jbn.2024.3856
Weizhong Yang, Lu Xu, Xiaohong Qin
{"title":"应用MicroRNA-124载体脂质体纳米粒子通过靶向BECN1抑制胰腺癌细胞进展和抑制自噬","authors":"Weizhong Yang, Lu Xu, Xiaohong Qin","doi":"10.1166/jbn.2024.3856","DOIUrl":null,"url":null,"abstract":"This study determines the efficacy of microRNA (miR)-124-loaded liposome nanoparticles on pancreatic cancer (PC). Herein, pancreatic cells were co-cultured with miR-124-loaded nanoparticles, pure liposome nanoparticles (empty vector group) or cultured alone (control group). The cells\n were administered with BECN1 inhibitor, and negative controls. The expression of autophagy-related factors (BECN1, P62, LC3) was determined by Western blot and cancer cell migration capacity was assessed by Transwell assay. The relation of miR-124 with BECN1 was assessed by bioinformatics\n analysis and dual-luciferase reporter gene assay. Compared with control group and the empty vector group, treatment with miR-124-loaded nanoparticles resulted in reduced number of migrated cells, scratch rate, and decreased expression of BECN1, P62, and LC3 (P < 0.05) without difference\n between control group and empty vector group (P > 0.05). Additional administration of BECN1 inhibitor further decreased migration and invasion of PC cells and obtained lower level of BECN1, P62, and LC3 protein, which was significantly lower than control group and miR-124+BECN1 NC\n group (P < 0.05). miR-124+BECN1 NC group exhibited lower expressions of BECN1, P62, and LC3 than control group (P < 0.05). Mechanistically, miR-124 targeted BECN1 to influence biological behaviors of PC cells. There is a target relationship between miR-124 and BECN1 in\n PC. miR-124-loaded nanoparticles incorporated with BECN1 inhibitor restrained autophagy through down-regulation of BECN1, P62, and LC3 and suppressed PC cell invasion and migration. These findings provide a novel insight into targeted therapy for PC.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9000,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Application of MicroRNA-124-Loaded Liposome Nanoparticles for Suppressing Pancreatic Cancer Cell Progression and Restraining Autophagy Through Targeting BECN1\",\"authors\":\"Weizhong Yang, Lu Xu, Xiaohong Qin\",\"doi\":\"10.1166/jbn.2024.3856\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This study determines the efficacy of microRNA (miR)-124-loaded liposome nanoparticles on pancreatic cancer (PC). Herein, pancreatic cells were co-cultured with miR-124-loaded nanoparticles, pure liposome nanoparticles (empty vector group) or cultured alone (control group). The cells\\n were administered with BECN1 inhibitor, and negative controls. The expression of autophagy-related factors (BECN1, P62, LC3) was determined by Western blot and cancer cell migration capacity was assessed by Transwell assay. The relation of miR-124 with BECN1 was assessed by bioinformatics\\n analysis and dual-luciferase reporter gene assay. Compared with control group and the empty vector group, treatment with miR-124-loaded nanoparticles resulted in reduced number of migrated cells, scratch rate, and decreased expression of BECN1, P62, and LC3 (P < 0.05) without difference\\n between control group and empty vector group (P > 0.05). Additional administration of BECN1 inhibitor further decreased migration and invasion of PC cells and obtained lower level of BECN1, P62, and LC3 protein, which was significantly lower than control group and miR-124+BECN1 NC\\n group (P < 0.05). miR-124+BECN1 NC group exhibited lower expressions of BECN1, P62, and LC3 than control group (P < 0.05). Mechanistically, miR-124 targeted BECN1 to influence biological behaviors of PC cells. There is a target relationship between miR-124 and BECN1 in\\n PC. miR-124-loaded nanoparticles incorporated with BECN1 inhibitor restrained autophagy through down-regulation of BECN1, P62, and LC3 and suppressed PC cell invasion and migration. These findings provide a novel insight into targeted therapy for PC.\",\"PeriodicalId\":15260,\"journal\":{\"name\":\"Journal of biomedical nanotechnology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2024-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of biomedical nanotechnology\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1166/jbn.2024.3856\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of biomedical nanotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1166/jbn.2024.3856","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0

摘要

本研究确定了载入微RNA(miR)-124的脂质体纳米颗粒对胰腺癌(PC)的疗效。在本研究中,胰腺细胞与miR-124负载纳米颗粒、纯脂质体纳米颗粒(空载体组)或单独培养(对照组)共同培养。细胞加入 BECN1 抑制剂和阴性对照组。用 Western 印迹法测定自噬相关因子(BECN1、P62、LC3)的表达,用 Transwell 试验评估癌细胞迁移能力。通过生物信息学分析和双荧光素酶报告基因检测评估了 miR-124 与 BECN1 的关系。与对照组和空载体组相比,miR-124负载纳米颗粒处理后,迁移细胞数量减少,划痕率降低,BECN1、P62和LC3的表达减少(P < 0.05),而对照组和空载体组之间无差异(P > 0.05)。miR-124+BECN1 NC组的BECN1、P62和LC3蛋白表达低于对照组和空载体组(P < 0.05)。从机制上看,miR-124靶向BECN1影响了PC细胞的生物学行为。miR-124与BECN1在PC中存在靶向关系。miR-124纳米颗粒与BECN1抑制剂结合,通过下调BECN1、P62和LC3抑制自噬,并抑制PC细胞的侵袭和迁移。这些发现为PC的靶向治疗提供了新的视角。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Application of MicroRNA-124-Loaded Liposome Nanoparticles for Suppressing Pancreatic Cancer Cell Progression and Restraining Autophagy Through Targeting BECN1
This study determines the efficacy of microRNA (miR)-124-loaded liposome nanoparticles on pancreatic cancer (PC). Herein, pancreatic cells were co-cultured with miR-124-loaded nanoparticles, pure liposome nanoparticles (empty vector group) or cultured alone (control group). The cells were administered with BECN1 inhibitor, and negative controls. The expression of autophagy-related factors (BECN1, P62, LC3) was determined by Western blot and cancer cell migration capacity was assessed by Transwell assay. The relation of miR-124 with BECN1 was assessed by bioinformatics analysis and dual-luciferase reporter gene assay. Compared with control group and the empty vector group, treatment with miR-124-loaded nanoparticles resulted in reduced number of migrated cells, scratch rate, and decreased expression of BECN1, P62, and LC3 (P < 0.05) without difference between control group and empty vector group (P > 0.05). Additional administration of BECN1 inhibitor further decreased migration and invasion of PC cells and obtained lower level of BECN1, P62, and LC3 protein, which was significantly lower than control group and miR-124+BECN1 NC group (P < 0.05). miR-124+BECN1 NC group exhibited lower expressions of BECN1, P62, and LC3 than control group (P < 0.05). Mechanistically, miR-124 targeted BECN1 to influence biological behaviors of PC cells. There is a target relationship between miR-124 and BECN1 in PC. miR-124-loaded nanoparticles incorporated with BECN1 inhibitor restrained autophagy through down-regulation of BECN1, P62, and LC3 and suppressed PC cell invasion and migration. These findings provide a novel insight into targeted therapy for PC.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
4.30
自引率
17.20%
发文量
145
审稿时长
2.3 months
期刊介绍: Information not localized
期刊最新文献
Mechanism of miR-126 Loaded in Albumin Nanoparticles for Reversing the Multidrug Resistance in Breast Carcinoma Cells Application of 20(S)-Protopanaxadiol-Loaded Nanostructured Lipid Carriers for Diabetic Wound Healing and Vascular Regeneration LncRNA NEAT1 Promotes the Cancer Stem Cell-Like Properties of HCC by miR-128-3p/GP73 Axis Pterostilbene-Loaded Polydopamine Nanoparticles Down-Regulate Tumor Necrosis Factor-α and Improve Myocardial Function in Mice with Acute Myocardial Infarction Phacoemulsification Plus Intraocular Lens Implantation with Gold Nanoparticles for Complicated Cataract Secondary to Uveitis: Efficacy Analysis
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1