与正常能繁母牛相比,长期不孕重复配种母牛的子宫炎症微环境

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Veterinary and Animal Science Pub Date : 2024-06-01 DOI:10.1016/j.vas.2024.100369
Maho Taru , Taiga Katoh , Karen Koshimizu , Sohei Kuribayashi , Ryotaro Miura , Seizo Hamano , Koumei Shirasuna
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引用次数: 0

摘要

泌乳奶牛的繁殖性能正在逐渐下降,造成这一问题的原因之一是存在长期不孕的重复种牛(RBCs)。RBC的成因主要被认为是母体因素,包括子宫环境。本研究旨在利用子宫组织和子宫液准确调查 RBC 的子宫环境。通过冲洗子宫收集子宫液,并在发情周期第 7 天通过活检收集正常育成牛和 RBC 牛(每组 5 头)的子宫内膜组织。对子宫的综合分析表明,正常能繁母牛和红细胞的基因表达和改变途径存在差异。特别是在 RBC 的子宫组织中发现了自然杀伤细胞介导的细胞毒性、细胞周期和钙信号通路。在子宫液中,RBC 的脂多糖含量高于正常组(P = 0.08)。在体外实验中,用 RBC 子宫液处理会上调牛子宫内膜上皮细胞中与炎症相关的通路和分子,如白细胞介素-8(IL-8)。总之,研究发现 RBC 的子宫环境处于炎症状态,导致繁殖性能降低。有必要制定方法来改善 RBC 子宫环境的抗炎状态。
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Inflammatory uterine microenvironment in long-term infertility repeat breeder cows compared with normal fertile cows

The reproductive performance of lactating dairy cows is gradually declining, and one of the causes of this problem is the presence of long-term infertility repeat breeder cows (RBCs). The causes of RBCs are largely thought to be maternal factors, including the uterine environment. This study aimed to accurately investigate the uterine environment of RBCs using uterine tissue and fluid. Next, we investigated the effect of nobiletin in bovine endometrial epithelial cells to explore the possibility of improving the uterine environment of RBCs.

Uterine fluid was collected by flushing the uterus and endometrial tissues were collected by biopsy on day 7 of the estrous cycle from both normal fertile cows and RBCs (n = 5 in each group). A comprehensive analysis of the uterus revealed that gene expression and altered pathways differed between normal fertile cows and RBCs. Especially, pathways of natural killer cell-mediated cytotoxicity, cell cycle, and calcium signaling pathway were picked up in the uterine tissues of RBCs. In the uterine fluid, the levels of lipopolysaccharide were higher in the RBC than in normal group (P = 0.08). In in vitro experiment, treatment with the uterine fluid from RBCs upregulated inflammation-related pathways and molecules such as interleukin-8 (IL-8) in bovine endometrial epithelial cells. The treatment with nobiletin suppressed IL-8 induced by the treatment with uterine fluid.

In conclusion, the uterine environment of RBCs was found to be in inflammatory condition, causing the lower reproductive performance. It is necessary to develop methods to improve to the anti-inflammatory state in the uterine environment of RBCs.

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来源期刊
Veterinary and Animal Science
Veterinary and Animal Science Veterinary-Veterinary (all)
CiteScore
3.50
自引率
0.00%
发文量
43
审稿时长
47 days
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