CRISPR/Cas9诱导的LEAP2和GHSR1a基因敲除突变斑马鱼表现出生长异常和脂质代谢障碍。

IF 2.1 3区 医学 Q3 ENDOCRINOLOGY & METABOLISM General and comparative endocrinology Pub Date : 2024-06-01 DOI:10.1016/j.ygcen.2024.114563
Yueyue Fei , Zhonggui Bao , Qin Wang , Yihong Zhu , Jigang Lu , Linyue Ouyang , Quiqin Hu , Yan Zhou , Liangbiao Chen
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引用次数: 0

摘要

探究鱼类脂肪沉积的原理并开展相关研究是当前鱼类营养学的重点。本研究通过构建突变模型,探讨内分泌因子LEAP2及其受体GHSR1a对斑马鱼生长、摄食和肝脏脂肪沉积的影响,从而探索斑马鱼LEAP2和GHSR1a的内分泌调控。与野生型(WT)相比,LEAP2 的突变会导致斑马鱼摄食增加和游泳减少。这些研究结果表明,LEAP2 和 GHSR1a 在斑马鱼的摄食、生长和细胞内脂肪代谢中起着至关重要的作用。该研究首次在模式生物斑马鱼体内研究了LEAP2和GHSR1a的内分泌代谢调节功能,初步揭示了它们对斑马鱼脂肪代谢的影响和潜在机制。
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CRISPR/Cas9-induced LEAP2 and GHSR1a knockout mutant zebrafish displayed abnormal growth and impaired lipid metabolism

Investigating the principles of fish fat deposition and conducting related research are current focal points in fish nutrition. This study explores the endocrine regulation of LEAP2 and GHSR1a in zebrafish by constructing mutant models and examining the effects of the endocrine factors LEAP2 and its receptor GHSR1a on zebrafish growth, feeding, and liver fat deposition. Compared to the wild type (WT), the mutation of LEAP2 results in increased feeding and decreased swimming in zebrafish. The impact is more pronounced in adult female zebrafish, characterized by increased weight, length, width, and accumulation of lipid droplets in the liver. In contrast, deficiency in GHSR1a significantly reduces the growth of male zebrafish and markedly decreases liver fat deposition. These research findings indicate the crucial roles of LEAP2 and GHSR1a in zebrafish feeding, growth, and intracellular fat metabolism. This study, for the first time, investigated the endocrine metabolic regulation functions of LEAP2 and GHSR1a in the model organism zebrafish, providing initial insights into their effects and potential mechanisms on zebrafish fat metabolism.

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来源期刊
General and comparative endocrinology
General and comparative endocrinology 医学-内分泌学与代谢
CiteScore
5.60
自引率
7.40%
发文量
120
审稿时长
2 months
期刊介绍: General and Comparative Endocrinology publishes articles concerned with the many complexities of vertebrate and invertebrate endocrine systems at the sub-molecular, molecular, cellular and organismal levels of analysis.
期刊最新文献
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