软组织愈合过程中抗氧化凝胶与洗必泰的效果比较:一项动物研究。

IF 4.2 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Journal of periodontology Pub Date : 2024-06-03 DOI:10.1002/JPER.23-0794
Kelechi Ukaegbu, Deborah Foyle, Xianghong Luan, Emet Schneiderman, Edward P Allen, Jacqueline Plemons, Kathy K H Svoboda
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引用次数: 0

摘要

背景:长期的炎症和氧化应激会阻碍伤口愈合。为了提高愈合效率,人们采用了许多解决方案。这是一项比较洗必泰(CHX)和商用抗氧化凝胶(AO)的体内研究:方法:在 60 只 Sprague-Dawley 大鼠的下门牙牙龈区域制作包膜瓣,并插入非细胞真皮基质 (ADM)。动物被随机分配到手术后使用 AO 凝胶或 0.12% CHX 的治疗组,每天两次。对照组不接受术后治疗。收集的数据(手术前、24 小时和 72 小时)包括手术图像、组织样本和体重。双盲评分员使用伤口愈合量表对图像进行评估。实时聚合酶链反应(RT-PCR)用于检测肿瘤坏死因子-α(TNFα)、白细胞介素-1(IL-1)、髓过氧化物酶(MPO)和超氧化物歧化酶(SOD)的基因表达:在临床评估中,AO 组的得分高于 CHX 组和对照组(p 结论:用 AO 治疗的动物痊愈了:接受 AO 治疗的动物痊愈得更快。AO 导致 TNFα 和抗氧化酶 SOD 提前上调。我们推测,AO 在通过 SOD 增加抗氧化反应来抵消氧化应激的同时,还促进了炎症进程的提前。
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The effect of an antioxidant gel compared to chlorhexidine during the soft tissue healing process: An animal study.

Background: Prolonged inflammation and oxidative stress can impede healing. To enhance healing efficiency, many solutions have been employed. This is an in vivo study comparing chlorhexidine (CHX) to a commercial antioxidant gel (AO).

Methods: Envelope flaps were created in the lower incisor gingival region of 60 Sprague-Dawley rats, and acellular dermal matrix (ADM) was inserted. Animals were randomly assigned to postsurgical treatment application of AO gel or 0.12% CHX twice daily. A control group received no postsurgical treatment. Data collected (before surgery, 24 h, and 72 h) included surgical images, tissue samples, and weights. Blinded scorers assessed images using a wound healing scale. Real-time polymerase chain reaction (RT-PCR) was used for gene expression of tumor necrosis factor-alpha (TNFα), interleukin-1 (IL-1), myeloperoxidase (MPO), and superoxide dismutase (SOD).

Results: The AO group scored higher than the CHX and control groups in clinical evaluation (p < 0.05). At 24 h, TNFα expression was upregulated in the AO group compared to CHX (p = 0.027) and controls (p = 0.018). The AO group had significantly higher expression of antioxidant enzyme (SOD) at 24 h compared to CHX (p = 0.021). All animals lost weight in the first 24 h. Animals treated with AO or CHX regained more weight at 72 h than control animals (p = 0.034 and 0.003, respectively).

Conclusion: Animals treated with AO healed faster. AO led to earlier upregulation of TNFα and antioxidant enzyme SOD. We hypothesized that AO promoted an earlier inflammatory process while counteracting oxidative stress by increasing antioxidant responses via SOD.

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来源期刊
Journal of periodontology
Journal of periodontology 医学-牙科与口腔外科
CiteScore
9.10
自引率
7.00%
发文量
290
审稿时长
3-8 weeks
期刊介绍: The Journal of Periodontology publishes articles relevant to the science and practice of periodontics and related areas.
期刊最新文献
Appraising the life-course impact of Epstein-Barr virus exposure and its genetic signature on periodontitis. Effectiveness of nonsurgical re‐instrumentation: Tooth‐related factors ISSUE INFORMATION Gingival inflammation and leukocyte-endothelium cell interactions in women with polycystic ovary syndrome. Clinical outcomes of using operating microscope for alveolar ridge preservation: A randomized controlled trial.
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