Chen He , Hua Wang , Li Fu , Qi Wang , Yingfang Zhong , Mimi Zeng , Xiaofeng Lin , Junyun Huang , Jun Xie , Qitong Huang , Min Yang
{"title":"用于体外监测γ-谷氨酰转肽酶活性的简单异构吡啶荧光探针:设计、合成和应用","authors":"Chen He , Hua Wang , Li Fu , Qi Wang , Yingfang Zhong , Mimi Zeng , Xiaofeng Lin , Junyun Huang , Jun Xie , Qitong Huang , Min Yang","doi":"10.1016/j.dyepig.2024.112252","DOIUrl":null,"url":null,"abstract":"<div><p>γ-Glutamyltranspeptidase (GGT) is a cell surface-associated enzyme, which has been proven to be closely related to many diseases. Thus, development of simple and effective GGT-activatable fluorescent probes for early diagnosis of diseases is of great significance. Herein, a series of simple isomeric pyridine-based GGT-activatable fluorescent probes has been successfully designed and synthesized. The preliminary experimental results indicate that <strong>Py-GGT-1</strong> has a significant fluorescence response to GGT via a rare fluorescence-off approach. Further fluorescence response experiments, such as the time-/dose-dependent fluorescence change and the selectivity/specificity estimation, reveal that <strong>Py-GGT-1</strong> has an excellent ability to monitor endogenous GGT activity. Mechanism studies through HRMS analysis, DFT calculations and molecular docking have theoretically verified the catalytic process between <strong>Py-GGT-1</strong> and GGT. To develop its practical application, live-cell imaging and serum-sample analysis were carried out, demonstrating that <strong>Py-GGT-1</strong> coud effectively track GGT within cells and organisms. Based its high selectivity and reasonable sensitivity, we expect that the probe will be applied in clinical diagnosis of GGT-related diseases in the future.</p></div>","PeriodicalId":302,"journal":{"name":"Dyes and Pigments","volume":null,"pages":null},"PeriodicalIF":4.1000,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Simple isomeric pyridine-based fluorescence-off probes for in vitro monitoring γ-glutamyltranspeptidase activity: Design, synthesis and application\",\"authors\":\"Chen He , Hua Wang , Li Fu , Qi Wang , Yingfang Zhong , Mimi Zeng , Xiaofeng Lin , Junyun Huang , Jun Xie , Qitong Huang , Min Yang\",\"doi\":\"10.1016/j.dyepig.2024.112252\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>γ-Glutamyltranspeptidase (GGT) is a cell surface-associated enzyme, which has been proven to be closely related to many diseases. Thus, development of simple and effective GGT-activatable fluorescent probes for early diagnosis of diseases is of great significance. Herein, a series of simple isomeric pyridine-based GGT-activatable fluorescent probes has been successfully designed and synthesized. The preliminary experimental results indicate that <strong>Py-GGT-1</strong> has a significant fluorescence response to GGT via a rare fluorescence-off approach. Further fluorescence response experiments, such as the time-/dose-dependent fluorescence change and the selectivity/specificity estimation, reveal that <strong>Py-GGT-1</strong> has an excellent ability to monitor endogenous GGT activity. Mechanism studies through HRMS analysis, DFT calculations and molecular docking have theoretically verified the catalytic process between <strong>Py-GGT-1</strong> and GGT. To develop its practical application, live-cell imaging and serum-sample analysis were carried out, demonstrating that <strong>Py-GGT-1</strong> coud effectively track GGT within cells and organisms. Based its high selectivity and reasonable sensitivity, we expect that the probe will be applied in clinical diagnosis of GGT-related diseases in the future.</p></div>\",\"PeriodicalId\":302,\"journal\":{\"name\":\"Dyes and Pigments\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.1000,\"publicationDate\":\"2024-05-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Dyes and Pigments\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0143720824003176\",\"RegionNum\":3,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, APPLIED\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Dyes and Pigments","FirstCategoryId":"88","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0143720824003176","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
Simple isomeric pyridine-based fluorescence-off probes for in vitro monitoring γ-glutamyltranspeptidase activity: Design, synthesis and application
γ-Glutamyltranspeptidase (GGT) is a cell surface-associated enzyme, which has been proven to be closely related to many diseases. Thus, development of simple and effective GGT-activatable fluorescent probes for early diagnosis of diseases is of great significance. Herein, a series of simple isomeric pyridine-based GGT-activatable fluorescent probes has been successfully designed and synthesized. The preliminary experimental results indicate that Py-GGT-1 has a significant fluorescence response to GGT via a rare fluorescence-off approach. Further fluorescence response experiments, such as the time-/dose-dependent fluorescence change and the selectivity/specificity estimation, reveal that Py-GGT-1 has an excellent ability to monitor endogenous GGT activity. Mechanism studies through HRMS analysis, DFT calculations and molecular docking have theoretically verified the catalytic process between Py-GGT-1 and GGT. To develop its practical application, live-cell imaging and serum-sample analysis were carried out, demonstrating that Py-GGT-1 coud effectively track GGT within cells and organisms. Based its high selectivity and reasonable sensitivity, we expect that the probe will be applied in clinical diagnosis of GGT-related diseases in the future.
期刊介绍:
Dyes and Pigments covers the scientific and technical aspects of the chemistry and physics of dyes, pigments and their intermediates. Emphasis is placed on the properties of the colouring matters themselves rather than on their applications or the system in which they may be applied.
Thus the journal accepts research and review papers on the synthesis of dyes, pigments and intermediates, their physical or chemical properties, e.g. spectroscopic, surface, solution or solid state characteristics, the physical aspects of their preparation, e.g. precipitation, nucleation and growth, crystal formation, liquid crystalline characteristics, their photochemical, ecological or biological properties and the relationship between colour and chemical constitution. However, papers are considered which deal with the more fundamental aspects of colourant application and of the interactions of colourants with substrates or media.
The journal will interest a wide variety of workers in a range of disciplines whose work involves dyes, pigments and their intermediates, and provides a platform for investigators with common interests but diverse fields of activity such as cosmetics, reprographics, dye and pigment synthesis, medical research, polymers, etc.