{"title":"外源脯氨酸调节果胶脱甲基化，从六价铬毒性中拯救细胞壁果胶甲基酯酶功能的水稻植株","authors":"Abid Ullah, Yu-Juan Lin, Peng Tian, Xiao-Zhang Yu","doi":"10.1186/s40538-024-00603-y","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Plants are equipped with several sophisticated mechanisms to deal with heavy metals (HMs) toxicity. Cell walls, which are rich in pectin, are important in the sequestration and compartmentalization of HMs. Pectin demethylation is carried out by pectin methylesterase (PME), which is a crucial activity in cell walls for the adsorption of HMs. This study focused on the factors that contribute to chromium (Cr) adsorption in rice plants exposed to Cr(VI) treatments without proline (Pro) “Cr(VI)” and with Pro “Pro + Cr(VI)” application.</p><h3>Results</h3><p>The results exhibited that when rice plants were treated with Cr(VI), their PME activity decreased, because Cr(VI) was bound to certain isoforms of PME and prevented the demethylation of pectin. The application of Pro increased PME activity by promoting the transcription of several PME-related genes. These genes were recognized on the basis of their similarity with PME genes in <i>Arabidopsis</i>. Gene expression variation factors (<i>GEVFs</i>) between the “Cr(VI)” and “Pro + Cr(VI)” treatments revealed that <i>OsPME7</i> and <i>OsPME9</i> have the highest positive <i>GEVF</i> values than other <i>OsPME</i> genes of rice. In addition, Pro application increased pectin content significantly in rice plants exposed to Cr(VI) stress. Proline application also leads to an increased concentration of Cr in rice roots compared with “Cr(VI)” treatments alone.</p><h3>Conclusions</h3><p>These findings suggest that Pro increased Cr(VI) adsorption in cell walls of rice plants by enhancing the PME activity and pectin content when exposed to “Cr(VI)” treatments, mainly regulated by <i>OsPME7</i> and <i>OsPME9</i>.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":512,"journal":{"name":"Chemical and Biological Technologies in Agriculture","volume":null,"pages":null},"PeriodicalIF":5.2000,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://chembioagro.springeropen.com/counter/pdf/10.1186/s40538-024-00603-y","citationCount":"0","resultStr":"{\"title\":\"Exogenous proline regulates pectin demethylation by rescuing pectin methylesterase functioning of cell wall from Cr(VI) toxicity in rice plants\",\"authors\":\"Abid Ullah, Yu-Juan Lin, Peng Tian, Xiao-Zhang Yu\",\"doi\":\"10.1186/s40538-024-00603-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Plants are equipped with several sophisticated mechanisms to deal with heavy metals (HMs) toxicity. Cell walls, which are rich in pectin, are important in the sequestration and compartmentalization of HMs. Pectin demethylation is carried out by pectin methylesterase (PME), which is a crucial activity in cell walls for the adsorption of HMs. This study focused on the factors that contribute to chromium (Cr) adsorption in rice plants exposed to Cr(VI) treatments without proline (Pro) “Cr(VI)” and with Pro “Pro + Cr(VI)” application.</p><h3>Results</h3><p>The results exhibited that when rice plants were treated with Cr(VI), their PME activity decreased, because Cr(VI) was bound to certain isoforms of PME and prevented the demethylation of pectin. The application of Pro increased PME activity by promoting the transcription of several PME-related genes. These genes were recognized on the basis of their similarity with PME genes in <i>Arabidopsis</i>. Gene expression variation factors (<i>GEVFs</i>) between the “Cr(VI)” and “Pro + Cr(VI)” treatments revealed that <i>OsPME7</i> and <i>OsPME9</i> have the highest positive <i>GEVF</i> values than other <i>OsPME</i> genes of rice. In addition, Pro application increased pectin content significantly in rice plants exposed to Cr(VI) stress. Proline application also leads to an increased concentration of Cr in rice roots compared with “Cr(VI)” treatments alone.</p><h3>Conclusions</h3><p>These findings suggest that Pro increased Cr(VI) adsorption in cell walls of rice plants by enhancing the PME activity and pectin content when exposed to “Cr(VI)” treatments, mainly regulated by <i>OsPME7</i> and <i>OsPME9</i>.</p><h3>Graphical Abstract</h3>\\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":512,\"journal\":{\"name\":\"Chemical and Biological Technologies in Agriculture\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.2000,\"publicationDate\":\"2024-06-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://chembioagro.springeropen.com/counter/pdf/10.1186/s40538-024-00603-y\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chemical and Biological Technologies in Agriculture\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://link.springer.com/article/10.1186/s40538-024-00603-y\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical and Biological Technologies in Agriculture","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1186/s40538-024-00603-y","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
摘要
背景植物具有多种复杂的机制来应对重金属（HMs）的毒性。富含果胶的细胞壁对重金属的封存和分隔非常重要。果胶去甲基化由果胶甲基酯酶（PME）完成，这是细胞壁吸附 HMs 的关键活动。结果表明，当水稻植株受到六价铬处理时，其果胶甲基化酶活性降低，这是因为六价铬与果胶甲基化酶的某些异构体结合，阻止了果胶的去甲基化。施用 Pro 可促进几个与 PME 相关基因的转录，从而提高 PME 的活性。这些基因与拟南芥中的 PME 基因相似。在 "Cr(VI) "和 "Pro + Cr(VI) "处理之间的基因表达变异系数（GEVFs）显示，OsPME7 和 OsPME9 的正 GEVF 值高于水稻的其他 OsPME 基因。此外，施用 Pro 能显著增加暴露于六价铬胁迫的水稻植株的果胶含量。结论 这些研究结果表明，当水稻植物暴露于 "六价铬 "胁迫时，施用 Pro 可通过提高 PME 活性和果胶含量来增加其细胞壁对六价铬的吸附，这主要是受 OsPME7 和 OsPME9 的调控。
Exogenous proline regulates pectin demethylation by rescuing pectin methylesterase functioning of cell wall from Cr(VI) toxicity in rice plants
Background
Plants are equipped with several sophisticated mechanisms to deal with heavy metals (HMs) toxicity. Cell walls, which are rich in pectin, are important in the sequestration and compartmentalization of HMs. Pectin demethylation is carried out by pectin methylesterase (PME), which is a crucial activity in cell walls for the adsorption of HMs. This study focused on the factors that contribute to chromium (Cr) adsorption in rice plants exposed to Cr(VI) treatments without proline (Pro) “Cr(VI)” and with Pro “Pro + Cr(VI)” application.
Results
The results exhibited that when rice plants were treated with Cr(VI), their PME activity decreased, because Cr(VI) was bound to certain isoforms of PME and prevented the demethylation of pectin. The application of Pro increased PME activity by promoting the transcription of several PME-related genes. These genes were recognized on the basis of their similarity with PME genes in Arabidopsis. Gene expression variation factors (GEVFs) between the “Cr(VI)” and “Pro + Cr(VI)” treatments revealed that OsPME7 and OsPME9 have the highest positive GEVF values than other OsPME genes of rice. In addition, Pro application increased pectin content significantly in rice plants exposed to Cr(VI) stress. Proline application also leads to an increased concentration of Cr in rice roots compared with “Cr(VI)” treatments alone.
Conclusions
These findings suggest that Pro increased Cr(VI) adsorption in cell walls of rice plants by enhancing the PME activity and pectin content when exposed to “Cr(VI)” treatments, mainly regulated by OsPME7 and OsPME9.
期刊介绍：
Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture.
This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population.
Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.
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