Okoroike C. Ozoemena, Emmanuel Boateng and Aicheng Chen
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引用次数: 0
摘要
心血管疾病是导致全球过早死亡的主要原因之一,而测定人体血清中的 C 反应蛋白(CRP)对疾病的诊断至关重要。在这项研究中,我们开发了一种基于洋葱样碳@聚丙烯腈(OLC-PAN)的电化学免疫传感器,用于检测 CRP 抗原。这是通过将 CRP 抗体固定在改性玻璃碳电极(GCE)上实现的。为了评估 CRP 抗原的电化学检测效果,研究人员采用了多种电化学技术,如循环伏安法(CV)、方波伏安法(SWV)和电化学阻抗谱法(EIS)。这种超灵敏的 CRP 抗原检测方法在 -4.52 至 -12.05 g mL-1 范围内表现出非常好的对数曲线,检出限(LOD)为 0.9 fg mL-1。所开发的电化学免疫传感器具有高选择性、高灵敏度和高稳定性,有助于微型化的护理点应用和心血管疾病的高效诊断。
Ultrasensitive electrochemical immunosensor for the detection of C-reactive protein antigen†
Cardiovascular disease is one of the leading causes of premature death worldwide, and the determination of C-reactive protein (CRP) from human serum is of vital importance for the diagnosis of the disease. For this study, we have developed an electrochemical immunosensor based on onion-like carbon@polyacrylonitrile (OLC-PAN) for the detection of CRP antigens. This was accomplished by immobilizing CRP antibodies on a modified glassy carbon electrode (GCE). Several electrochemical techniques such as cyclic voltammetry (CV), square wave voltammetry (SWV), and electrochemical impedance spectroscopy (EIS) were employed to evaluate the electrochemical detection of the CRP antigen. This ultrasensitive method for CRP antigen detection exhibited a very good logarithmic plot from −4.52 to −12.05 g mL−1 and a limit of detection (LOD) of 0.9 fg mL−1. The high selectivity, sensitivity, and stability of the developed electrochemical immunosensor would facilitate miniaturization for point-of-care applications and the efficient diagnosis of cardiovascular diseases.