J. Martin de los Santos-Ramirez, Carlos A. Mendiola-Escobedo, Jose M. Cotera-Sarabia, Roberto C. Gallo-Villanueva, Rodrigo Martinez-Duarte and Victor H. Perez-Gonzalez
{"title":"利用低电压鉴定粒子的非线性电动特性","authors":"J. Martin de los Santos-Ramirez, Carlos A. Mendiola-Escobedo, Jose M. Cotera-Sarabia, Roberto C. Gallo-Villanueva, Rodrigo Martinez-Duarte and Victor H. Perez-Gonzalez","doi":"10.1039/D4AN00538D","DOIUrl":null,"url":null,"abstract":"<p >Insulator-based electrokinetically driven microfluidic devices stimulated with direct current (DC) voltages are an attractive solution for particle separation, concentration, or isolation. However, to design successful particle manipulation protocols, it is mandatory to know the mobilities of electroosmosis, and linear and nonlinear electrophoresis of the microchannel/liquid/particle system. Several techniques exist to characterize the mobilities of electroosmosis and linear electrophoresis. However, only one method to characterize the mobility of nonlinear electrophoresis has been thoroughly assessed, which generally requires DC voltages larger than 1000 V and measuring particle velocity in a straight microchannel. Under such conditions, Joule heating, electrolysis, and the DC power source cost become a concern. Also, measuring particle velocity at high voltages is noisy, limiting characterization quality. Here we present a protocol—tested on 2 μm polystyrene particles—for characterizing the mobility of nonlinear electrophoresis of the liquid/particle system using a DC voltage of only 30 V and visual inspection of particle dynamics in a microchannel featuring insulating obstacles. Multiphysics numerical modelling was used to guide microchannel design and to correlate particle location during an experiment with electric field intensity. The method was validated against the conventional characterization protocol, exhibiting excellent agreement while significantly reducing measurement noise and experimental complexity.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":null,"pages":null},"PeriodicalIF":3.6000,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Enabling the characterization of the nonlinear electrokinetic properties of particles using low voltage†\",\"authors\":\"J. Martin de los Santos-Ramirez, Carlos A. Mendiola-Escobedo, Jose M. Cotera-Sarabia, Roberto C. Gallo-Villanueva, Rodrigo Martinez-Duarte and Victor H. Perez-Gonzalez\",\"doi\":\"10.1039/D4AN00538D\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Insulator-based electrokinetically driven microfluidic devices stimulated with direct current (DC) voltages are an attractive solution for particle separation, concentration, or isolation. However, to design successful particle manipulation protocols, it is mandatory to know the mobilities of electroosmosis, and linear and nonlinear electrophoresis of the microchannel/liquid/particle system. Several techniques exist to characterize the mobilities of electroosmosis and linear electrophoresis. However, only one method to characterize the mobility of nonlinear electrophoresis has been thoroughly assessed, which generally requires DC voltages larger than 1000 V and measuring particle velocity in a straight microchannel. Under such conditions, Joule heating, electrolysis, and the DC power source cost become a concern. Also, measuring particle velocity at high voltages is noisy, limiting characterization quality. Here we present a protocol—tested on 2 μm polystyrene particles—for characterizing the mobility of nonlinear electrophoresis of the liquid/particle system using a DC voltage of only 30 V and visual inspection of particle dynamics in a microchannel featuring insulating obstacles. Multiphysics numerical modelling was used to guide microchannel design and to correlate particle location during an experiment with electric field intensity. The method was validated against the conventional characterization protocol, exhibiting excellent agreement while significantly reducing measurement noise and experimental complexity.</p>\",\"PeriodicalId\":63,\"journal\":{\"name\":\"Analyst\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.6000,\"publicationDate\":\"2024-06-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analyst\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://pubs.rsc.org/en/content/articlelanding/2024/an/d4an00538d\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analyst","FirstCategoryId":"92","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2024/an/d4an00538d","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
摘要
在直流电压的刺激下,基于绝缘体的电动微流控装置是一种极具吸引力的颗粒分离、浓缩或隔离解决方案。然而,要设计出成功的粒子操作方案,必须了解微通道/液体/粒子系统的电渗、线性和非线性电泳的流动性。有几种技术可以表征电渗和线性电泳的流动性。然而,只有一种表征非线性电泳流动性的方法经过了全面评估,这种方法通常需要大于 1000 V 的直流电压,并测量直微通道中粒子的速度。在这种条件下,焦耳热、电解和直流电源成本都会成为问题。此外,在高电压下测量粒子速度会产生噪声,从而限制了表征质量。在此,我们介绍了一种在 2 µm 聚苯乙烯颗粒上进行测试的方案,该方案利用仅 30 V 的直流电压和对微通道(具有绝缘障碍物)中颗粒动态的目测,来表征液体/颗粒系统非线性电泳的流动性。多物理场数值建模用于指导微通道设计,并将实验过程中粒子的位置与电场强度相关联。该方法与传统的表征规程进行了验证,显示出极佳的一致性,同时显著降低了测量噪声和实验复杂性。
Enabling the characterization of the nonlinear electrokinetic properties of particles using low voltage†
Insulator-based electrokinetically driven microfluidic devices stimulated with direct current (DC) voltages are an attractive solution for particle separation, concentration, or isolation. However, to design successful particle manipulation protocols, it is mandatory to know the mobilities of electroosmosis, and linear and nonlinear electrophoresis of the microchannel/liquid/particle system. Several techniques exist to characterize the mobilities of electroosmosis and linear electrophoresis. However, only one method to characterize the mobility of nonlinear electrophoresis has been thoroughly assessed, which generally requires DC voltages larger than 1000 V and measuring particle velocity in a straight microchannel. Under such conditions, Joule heating, electrolysis, and the DC power source cost become a concern. Also, measuring particle velocity at high voltages is noisy, limiting characterization quality. Here we present a protocol—tested on 2 μm polystyrene particles—for characterizing the mobility of nonlinear electrophoresis of the liquid/particle system using a DC voltage of only 30 V and visual inspection of particle dynamics in a microchannel featuring insulating obstacles. Multiphysics numerical modelling was used to guide microchannel design and to correlate particle location during an experiment with electric field intensity. The method was validated against the conventional characterization protocol, exhibiting excellent agreement while significantly reducing measurement noise and experimental complexity.