{"title":"卡累利阿桦木[Betula pendula Roth var. carelica (Mercl.) Hämet-Ahti]不同木质部发生情况下的 UPBEAT1-ROS-POD-PAL 系统。","authors":"Kseniya Mihajlovna Nikerova, Nataliya Alekseevna Galibina, Irina Nikolaevna Sofronova, Yuliya Leonidovna Moshchenskaya, Maksim Anatol'evich Korzhenevskij, Anna Vladimirovna Klimova, Tatiana Vladimirovna Tarelkina","doi":"10.2174/0109298665291781240529044444","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>We studied <i>UPBEAT1 (UPB1)</i> which regulated superoxide radical / hydrogen peroxide ratio together with peroxidase (POD) activity and <i>PAL</i> genes expression under different ways of apical meristem development during the xylem structural elements' formation in unique woody plants <i>B. pendula</i> var. pendula with straight-grained wood and <i>B. pendula</i> var. carelica with figured wood. The differentiation process predominanced in straight-grained wood (<i>B. pendula</i> var. <i>pendula</i>) or proliferation - in the figured wood. The investigation was conducted in the radial row (cambial zone - differentiating xylem - mature xylem) during the active cambial growth period.</p><p><strong>Objective: </strong>The study aimed to study the xylogenesis processes occurring in the 16-year-old straight-grained silver birch (<i>Betula pendula</i> Roth) and Karelian birch (<i>Betula pendula</i> Roth var. carelica (Mercl.) Hämet-Ahti) with figured wood.</p><p><strong>Methods: </strong>Hydrogen peroxide and superoxide radical contents and peroxidase activity were determined spectrophotometrically. Gene expression for <i>PAL</i> family genes and the <i>UPBEAT1</i> gene was assessed using qRT-PCR.</p><p><strong>Results: </strong>Principal component analysis has confirmed trees with straight-grained and figured wood to be different according to UPBEAT1-ROS-POD-<i>PAL</i> system functioning.</p><p><strong>Conclusion: </strong>The higher superoxide radical/hydrogen peroxide ratio in figured Karelian birch, along with <i>UPBEAT1</i> transcription factor and <i>PAL</i> genes upregulation, distinguished it from straight-grained silver birch. This metabolic picture confirmed the shift of Karelian birch xylogenesis towards proliferation processes, accompanied by ROS and phenolic compounds' flow and POD activity.</p>","PeriodicalId":20736,"journal":{"name":"Protein and Peptide Letters","volume":" ","pages":"375-385"},"PeriodicalIF":1.0000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"<i>UPBEAT1</i>-ROS-POD-<i>PAL</i> System under Different Xylogenesis Scenarios in Karelian Birch <i>(Betula pendula</i> Roth var. carelica (Mercl.) Hämet-Ahti).\",\"authors\":\"Kseniya Mihajlovna Nikerova, Nataliya Alekseevna Galibina, Irina Nikolaevna Sofronova, Yuliya Leonidovna Moshchenskaya, Maksim Anatol'evich Korzhenevskij, Anna Vladimirovna Klimova, Tatiana Vladimirovna Tarelkina\",\"doi\":\"10.2174/0109298665291781240529044444\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>We studied <i>UPBEAT1 (UPB1)</i> which regulated superoxide radical / hydrogen peroxide ratio together with peroxidase (POD) activity and <i>PAL</i> genes expression under different ways of apical meristem development during the xylem structural elements' formation in unique woody plants <i>B. pendula</i> var. pendula with straight-grained wood and <i>B. pendula</i> var. carelica with figured wood. The differentiation process predominanced in straight-grained wood (<i>B. pendula</i> var. <i>pendula</i>) or proliferation - in the figured wood. The investigation was conducted in the radial row (cambial zone - differentiating xylem - mature xylem) during the active cambial growth period.</p><p><strong>Objective: </strong>The study aimed to study the xylogenesis processes occurring in the 16-year-old straight-grained silver birch (<i>Betula pendula</i> Roth) and Karelian birch (<i>Betula pendula</i> Roth var. carelica (Mercl.) Hämet-Ahti) with figured wood.</p><p><strong>Methods: </strong>Hydrogen peroxide and superoxide radical contents and peroxidase activity were determined spectrophotometrically. Gene expression for <i>PAL</i> family genes and the <i>UPBEAT1</i> gene was assessed using qRT-PCR.</p><p><strong>Results: </strong>Principal component analysis has confirmed trees with straight-grained and figured wood to be different according to UPBEAT1-ROS-POD-<i>PAL</i> system functioning.</p><p><strong>Conclusion: </strong>The higher superoxide radical/hydrogen peroxide ratio in figured Karelian birch, along with <i>UPBEAT1</i> transcription factor and <i>PAL</i> genes upregulation, distinguished it from straight-grained silver birch. This metabolic picture confirmed the shift of Karelian birch xylogenesis towards proliferation processes, accompanied by ROS and phenolic compounds' flow and POD activity.</p>\",\"PeriodicalId\":20736,\"journal\":{\"name\":\"Protein and Peptide Letters\",\"volume\":\" \",\"pages\":\"375-385\"},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2024-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Protein and Peptide Letters\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.2174/0109298665291781240529044444\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Protein and Peptide Letters","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.2174/0109298665291781240529044444","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
研究背景本研究对具有直纹木质部的B. pendula变种和具有花纹木质部的B. pendula变种进行了研究，研究发现UPBEAT1（UPB1）在直纹木质部（B. pendula var.研究发现，分化过程在直纹木（B. pendula var.调查是在木质部生长活跃期对径向行（木质部分化区-木质部分化-成熟木质部）进行的：本研究旨在研究 16 年生直纹银桦（Betula pendula Roth）和卡累利阿桦（Betula pendula Roth var. carelica (Mercl.) Hämet-Ahti）花纹木的木质部生成过程：方法：用分光光度法测定过氧化氢和超氧自由基的含量以及过氧化物酶的活性。使用 qRT-PCR 评估 PAL 家族基因和 UPBEAT1 基因的表达：结果：主成分分析证实，根据 UPBEAT1-ROS-POD-PAL 系统的功能，直纹木和花纹木的树木是不同的：结论：花纹卡累利阿桦木的超氧自由基/过氧化氢比率较高，同时 UPBEAT1 转录因子和 PAL 基因上调，使其与直纹银桦区分开来。这种新陈代谢情况证实了卡累利阿桦木木质化过程转向增殖过程，同时伴随着 ROS 和酚类化合物的流动以及 POD 活性。
UPBEAT1-ROS-POD-PAL System under Different Xylogenesis Scenarios in Karelian Birch (Betula pendula Roth var. carelica (Mercl.) Hämet-Ahti).
Background: We studied UPBEAT1 (UPB1) which regulated superoxide radical / hydrogen peroxide ratio together with peroxidase (POD) activity and PAL genes expression under different ways of apical meristem development during the xylem structural elements' formation in unique woody plants B. pendula var. pendula with straight-grained wood and B. pendula var. carelica with figured wood. The differentiation process predominanced in straight-grained wood (B. pendula var. pendula) or proliferation - in the figured wood. The investigation was conducted in the radial row (cambial zone - differentiating xylem - mature xylem) during the active cambial growth period.
Objective: The study aimed to study the xylogenesis processes occurring in the 16-year-old straight-grained silver birch (Betula pendula Roth) and Karelian birch (Betula pendula Roth var. carelica (Mercl.) Hämet-Ahti) with figured wood.
Methods: Hydrogen peroxide and superoxide radical contents and peroxidase activity were determined spectrophotometrically. Gene expression for PAL family genes and the UPBEAT1 gene was assessed using qRT-PCR.
Results: Principal component analysis has confirmed trees with straight-grained and figured wood to be different according to UPBEAT1-ROS-POD-PAL system functioning.
Conclusion: The higher superoxide radical/hydrogen peroxide ratio in figured Karelian birch, along with UPBEAT1 transcription factor and PAL genes upregulation, distinguished it from straight-grained silver birch. This metabolic picture confirmed the shift of Karelian birch xylogenesis towards proliferation processes, accompanied by ROS and phenolic compounds' flow and POD activity.
期刊介绍：
Protein & Peptide Letters publishes letters, original research papers, mini-reviews and guest edited issues in all important aspects of protein and peptide research, including structural studies, advances in recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, and drug design. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallization and preliminary structure determination of biologically important proteins are considered only if they include significant new approaches or deal with proteins of immediate importance, and preliminary structure determinations of biologically important proteins. Purely theoretical/review papers should provide new insight into the principles of protein/peptide structure and function. Manuscripts describing computational work should include some experimental data to provide confirmation of the results of calculations.
Protein & Peptide Letters focuses on:
Structure Studies
Advances in Recombinant Expression
Drug Design
Chemical Synthesis
Function
Pharmacology
Enzymology
Conformational Analysis
Immunology
Biotechnology
Protein Engineering
Protein Folding
Sequencing
Molecular Recognition
Purification and Analysis
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