用阿联酋法和高效液相色谱分析法测定十种不同多酚化合物的化学含量

Levent Nuralın
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摘要

Shilajit(木犀草)是一种天然生物活性化合物复合物,历史上曾被用作治疗多种皮肤病的膏药,在传统医学中也适用于治疗糖尿病、老年痴呆症和癌症等疾病。在这项研究中,为了确定夏拉吉特的多酚含量,采用高效液相色谱-紫外法(HPLC-UV)对在 45 度下获得的提取物进行了分析,对 10 种不同的多酚化合物进行了定量分析。实验结果表明,常规含量为 28.99±1.23 µg/g,阿魏酸含量为 25.47±1.67 µg/g,白藜芦醇含量为 41.49±0.41 µg/g,紫杉醇含量为 532.19±8.21 µg/g。这项研究的结果将对生物活性物质的提取、规模研究及其作为食品补充剂和医药应用等领域做出重大贡献。
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Determination of the Chemical Content of Shilajit in Terms of Ten Different Polyphenolic Compounds by UAE Method and HPLC Analysis
Shilajit (Mumio) is a complex of natural bioactive compounds that has historically been used as a therapeutic cream for many skin diseases and is suitable in traditional medicine for diseases such as diabetes, Alzheimer's and cancer. In this study, to determine the polyphenolic content of Shilajit, extracts obtained at 45 degrees were analyzed by High Performance Liquid Chromatography-Ultraviolet (HPLC-UV) for the quantitative analysis of ten different polyphenolic compounds. As a result of the experimental study, 28.99±1.23 µg/g routine, 25.47±1.67 µg/g ferulic acid, 41.49±0.41 µg/g resveratrol and 532.19±8.21 µg/g taxifolin was obtained. This study's results will significantly contribute to sectors such as the extraction of bioactive substances, scaling studies, and their use as food supplements and pharmaceutical applications.
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