孟加拉国山羊肉市场上产谱β-内酰胺酶耐多药肠炎沙门氏菌血清型的探索

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Veterinary and Animal Science Pub Date : 2024-06-02 DOI:10.1016/j.vas.2024.100367
Jarin Al Naser , Hemayet Hossain , Md. Shahidur Rahman Chowdhury , Nasrin Akter Liza , Rayhan Mahmud Lasker , Asikur Rahman , Md. Ariful Haque , Md. Mukter Hossain , Md. Mahfujur Rahman
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引用次数: 0

摘要

能产生广谱β-内酰胺酶并表现出多重耐药性(MDR)的肠炎沙门氏菌血清型的出现对全球构成了巨大威胁,导致了广泛的食源性疾病,给公共卫生带来了令人担忧的问题。本研究特别集中于分离和鉴定 ESBL 耐药基因(blaTEM、blaSHV、blaCTX-M1、blaCTX-M2、blaCTX-M9、MultiCaseACC、MultiCaseMOX、MultiCaseDHA、blaOXA),以及对孟加拉国零售店山羊肉样本中发现的肠炎沙门氏菌血清型进行抗生素谱分析。在孟加拉国锡尔赫特地区进行研究期间,研究人员从 13 个不同的乡收集了共计 210 份山羊肉样本。研究人员主要采用培养和生化方法从所选样本中分离细菌。通过聚合酶链式反应 (PCR) 鉴定出了肠炎沙门氏菌血清 Typhimurium 和 Enteritidis 以及三种耐 ESBL 基因。采用盘扩散试验确定抗菌药敏感性。在分析的 210 个样本中,18.10%(210 个样本中有 38 个)检测到沙门氏菌属,9.05%(210 个样本中有 19 个)和 5.24%(210 个样本中有 11 个)的样本中分别检测到肠炎沙门氏菌和鼠伤寒沙门氏菌。共有 72.73 %(8/11)的肠炎双球菌和 100 %(19/19)的伤寒杆菌分离物呈耐多药模式阳性。伤寒杆菌的 blaTEM 基因检测结果呈阳性的占 63.16%(19 个中有 12 个),blaSHV 基因检测结果呈阳性的占 21.05%(4/19)。该研究认为,山羊肉零售市场渠道可能是产ESBL MDR肠炎沙门氏菌血清型的主要传播途径,对公共健康构成重大危害。
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Exploring of spectrum beta lactamase producing multidrug-resistant Salmonella enterica serovars in goat meat markets of Bangladesh

The emergence of Salmonella enterica serovars that produce extended-spectrum beta-lactamase and exhibit multi-drug resistance (MDR) poses a substantial global threat, contributing to widespread foodborne illnesses and presenting an alarming issue for public health. This study specifically concentrated on the isolation and identification of ESBL-resistant genes (blaTEM, blaSHV, blaCTX-M1, blaCTX-M2, blaCTX-M9, MultiCaseACC, MultiCaseMOX, MultiCaseDHA, blaOXA) and the antibiogram profiling of Salmonella enterica serovars found in goat meat samples procured from retail outlets in Bangladesh. During the research in the Sylhet district of Bangladesh, researchers gathered a total of 210 samples of goat meat from 13 different Upazilas. Primarily, cultural and biochemical methods were used for isolation of bacteria from the selected samples. Salmonella enterica serovars Typhimurium and Enteritidis, along with three ESBL-resistant genes, were identified through polymerase chain reactions (PCRs). The disk diffusion test was used to determine antimicrobial susceptibilities. Out of 210 samples analysed, Salmonella spp. was detected in 18.10 % (38 out of 210), with S. Enteritidis and S. Typhimurium found in 9.05 % (19 out of 210) and 5.24 % (11 out of 210) of the samples, respectively. A total of 72.73 % (8/11) of S. Enteritidis and 100 % (19/19) of S. Typhimurium isolates were positive by Multidrug-resistant patterns. The positive outcomes were found of S. Typhimurium tested 63.16 % (12 out of 19) for the blaTEM gene and 21.05 % (4/19) for the blaSHV, gene. The study proposes that the retail goat meat market channel could be a prominent transmission way of ESBL-producing MDR Salmonella enterica serovars, representing a significant public health hazard.

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来源期刊
Veterinary and Animal Science
Veterinary and Animal Science Veterinary-Veterinary (all)
CiteScore
3.50
自引率
0.00%
发文量
43
审稿时长
47 days
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