{"title":"孟加拉国山羊肉市场上产谱β-内酰胺酶耐多药肠炎沙门氏菌血清型的探索","authors":"Jarin Al Naser , Hemayet Hossain , Md. Shahidur Rahman Chowdhury , Nasrin Akter Liza , Rayhan Mahmud Lasker , Asikur Rahman , Md. Ariful Haque , Md. Mukter Hossain , Md. Mahfujur Rahman","doi":"10.1016/j.vas.2024.100367","DOIUrl":null,"url":null,"abstract":"<div><p>The emergence of <em>Salmonella enterica</em> serovars that produce extended-spectrum beta-lactamase and exhibit multi-drug resistance (MDR) poses a substantial global threat, contributing to widespread foodborne illnesses and presenting an alarming issue for public health. This study specifically concentrated on the isolation and identification of ESBL-resistant genes (<em>bla</em><sub>TEM,</sub> <em>bla</em><sub>SHV,</sub> <em>bla</em><sub>CTX-M1,</sub> <em>bla</em><sub>CTX-M2,</sub> <em>bla</em><sub>CTX-M9,</sub> <em>MultiCase</em><sub>ACC,</sub> <em>MultiCase</em><sub>MOX,</sub> <em>MultiCase</em><sub>DHA,</sub> <em>bla</em><sub>OXA</sub>) and the antibiogram profiling of <em>Salmonella enterica</em> serovars found in goat meat samples procured from retail outlets in Bangladesh. During the research in the Sylhet district of Bangladesh, researchers gathered a total of 210 samples of goat meat from 13 different Upazilas. Primarily, cultural and biochemical methods were used for isolation of bacteria from the selected samples. <em>Salmonella enterica</em> serovars Typhimurium and Enteritidis, along with three ESBL-resistant genes, were identified through polymerase chain reactions (PCRs). The disk diffusion test was used to determine antimicrobial susceptibilities. Out of 210 samples analysed, <em>Salmonella</em> spp<em>.</em> was detected in 18.10 % (38 out of 210), with <em>S.</em> Enteritidis and <em>S.</em> Typhimurium found in 9.05 % (19 out of 210) and 5.24 % (11 out of 210) of the samples, respectively. A total of 72.73 % (8/11) of <em>S.</em> Enteritidis and 100 % (19/19) of <em>S.</em> Typhimurium isolates were positive by Multidrug-resistant patterns. The positive outcomes were found of <em>S.</em> Typhimurium tested 63.16 % (12 out of 19) for the <em>bla</em><sub>TEM</sub> gene and 21.05 % (4/19) for the <em>bla</em><sub>SHV,</sub> gene. The study proposes that the retail goat meat market channel could be a prominent transmission way of ESBL-producing MDR <em>Salmonella enterica</em> serovars, representing a significant public health hazard.</p></div>","PeriodicalId":37152,"journal":{"name":"Veterinary and Animal Science","volume":null,"pages":null},"PeriodicalIF":1.9000,"publicationDate":"2024-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2451943X24000346/pdfft?md5=0fda53a5833e587404ff5adb1fd2dcdb&pid=1-s2.0-S2451943X24000346-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Exploring of spectrum beta lactamase producing multidrug-resistant Salmonella enterica serovars in goat meat markets of Bangladesh\",\"authors\":\"Jarin Al Naser , Hemayet Hossain , Md. Shahidur Rahman Chowdhury , Nasrin Akter Liza , Rayhan Mahmud Lasker , Asikur Rahman , Md. Ariful Haque , Md. Mukter Hossain , Md. Mahfujur Rahman\",\"doi\":\"10.1016/j.vas.2024.100367\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The emergence of <em>Salmonella enterica</em> serovars that produce extended-spectrum beta-lactamase and exhibit multi-drug resistance (MDR) poses a substantial global threat, contributing to widespread foodborne illnesses and presenting an alarming issue for public health. This study specifically concentrated on the isolation and identification of ESBL-resistant genes (<em>bla</em><sub>TEM,</sub> <em>bla</em><sub>SHV,</sub> <em>bla</em><sub>CTX-M1,</sub> <em>bla</em><sub>CTX-M2,</sub> <em>bla</em><sub>CTX-M9,</sub> <em>MultiCase</em><sub>ACC,</sub> <em>MultiCase</em><sub>MOX,</sub> <em>MultiCase</em><sub>DHA,</sub> <em>bla</em><sub>OXA</sub>) and the antibiogram profiling of <em>Salmonella enterica</em> serovars found in goat meat samples procured from retail outlets in Bangladesh. During the research in the Sylhet district of Bangladesh, researchers gathered a total of 210 samples of goat meat from 13 different Upazilas. Primarily, cultural and biochemical methods were used for isolation of bacteria from the selected samples. <em>Salmonella enterica</em> serovars Typhimurium and Enteritidis, along with three ESBL-resistant genes, were identified through polymerase chain reactions (PCRs). The disk diffusion test was used to determine antimicrobial susceptibilities. Out of 210 samples analysed, <em>Salmonella</em> spp<em>.</em> was detected in 18.10 % (38 out of 210), with <em>S.</em> Enteritidis and <em>S.</em> Typhimurium found in 9.05 % (19 out of 210) and 5.24 % (11 out of 210) of the samples, respectively. A total of 72.73 % (8/11) of <em>S.</em> Enteritidis and 100 % (19/19) of <em>S.</em> Typhimurium isolates were positive by Multidrug-resistant patterns. The positive outcomes were found of <em>S.</em> Typhimurium tested 63.16 % (12 out of 19) for the <em>bla</em><sub>TEM</sub> gene and 21.05 % (4/19) for the <em>bla</em><sub>SHV,</sub> gene. The study proposes that the retail goat meat market channel could be a prominent transmission way of ESBL-producing MDR <em>Salmonella enterica</em> serovars, representing a significant public health hazard.</p></div>\",\"PeriodicalId\":37152,\"journal\":{\"name\":\"Veterinary and Animal Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2024-06-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2451943X24000346/pdfft?md5=0fda53a5833e587404ff5adb1fd2dcdb&pid=1-s2.0-S2451943X24000346-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary and Animal Science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2451943X24000346\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary and Animal Science","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2451943X24000346","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
Exploring of spectrum beta lactamase producing multidrug-resistant Salmonella enterica serovars in goat meat markets of Bangladesh
The emergence of Salmonella enterica serovars that produce extended-spectrum beta-lactamase and exhibit multi-drug resistance (MDR) poses a substantial global threat, contributing to widespread foodborne illnesses and presenting an alarming issue for public health. This study specifically concentrated on the isolation and identification of ESBL-resistant genes (blaTEM,blaSHV,blaCTX-M1,blaCTX-M2,blaCTX-M9,MultiCaseACC,MultiCaseMOX,MultiCaseDHA,blaOXA) and the antibiogram profiling of Salmonella enterica serovars found in goat meat samples procured from retail outlets in Bangladesh. During the research in the Sylhet district of Bangladesh, researchers gathered a total of 210 samples of goat meat from 13 different Upazilas. Primarily, cultural and biochemical methods were used for isolation of bacteria from the selected samples. Salmonella enterica serovars Typhimurium and Enteritidis, along with three ESBL-resistant genes, were identified through polymerase chain reactions (PCRs). The disk diffusion test was used to determine antimicrobial susceptibilities. Out of 210 samples analysed, Salmonella spp. was detected in 18.10 % (38 out of 210), with S. Enteritidis and S. Typhimurium found in 9.05 % (19 out of 210) and 5.24 % (11 out of 210) of the samples, respectively. A total of 72.73 % (8/11) of S. Enteritidis and 100 % (19/19) of S. Typhimurium isolates were positive by Multidrug-resistant patterns. The positive outcomes were found of S. Typhimurium tested 63.16 % (12 out of 19) for the blaTEM gene and 21.05 % (4/19) for the blaSHV, gene. The study proposes that the retail goat meat market channel could be a prominent transmission way of ESBL-producing MDR Salmonella enterica serovars, representing a significant public health hazard.