大脑皮层星形胶质细胞的层状位置、形态和基因表达谱受脑室/脑室下祖细胞出生时间的影响。

IF 5.4 2区 医学 Q1 NEUROSCIENCES Glia Pub Date : 2024-06-09 DOI:10.1002/glia.24578
Daniela Lozano Casasbuenas, Ines Kortebi, Charles Gora, Erica Y. Scott, Celeste Gomes, Markley Silva Oliveira Jr, Tanvi Sharma, Emerson Daniele, Arman Olfat, Rachel Gibbs, Scott A. Yuzwa, Emily A. Gilbert, Patrick Küry, Aaron R. Wheeler, Martin Lévesque, Maryam Faiz
{"title":"大脑皮层星形胶质细胞的层状位置、形态和基因表达谱受脑室/脑室下祖细胞出生时间的影响。","authors":"Daniela Lozano Casasbuenas,&nbsp;Ines Kortebi,&nbsp;Charles Gora,&nbsp;Erica Y. Scott,&nbsp;Celeste Gomes,&nbsp;Markley Silva Oliveira Jr,&nbsp;Tanvi Sharma,&nbsp;Emerson Daniele,&nbsp;Arman Olfat,&nbsp;Rachel Gibbs,&nbsp;Scott A. Yuzwa,&nbsp;Emily A. Gilbert,&nbsp;Patrick Küry,&nbsp;Aaron R. Wheeler,&nbsp;Martin Lévesque,&nbsp;Maryam Faiz","doi":"10.1002/glia.24578","DOIUrl":null,"url":null,"abstract":"<p>Astrocytes that reside in superficial (SL) and deep cortical layers have distinct molecular profiles and morphologies, which may underlie specific functions. Here, we demonstrate that the production of SL and deep layer (DL) astrocyte populations from neural progenitor cells in the mouse is temporally regulated. Lineage tracking following <i>in utero</i> and postnatal electroporation with <i>PiggyBac</i> (<i>PB</i>) EGFP and birth dating with EdU and FlashTag, showed that apical progenitors produce astrocytes during late embryogenesis (E16.5) that are biased to the SL, while postnatally labeled (P0) astrocytes are biased to the DL. In contrast, astrocytes born during the predominantly neurogenic window (E14.5) showed a random distribution in the SL and DL. Of interest, E13.5 astrocytes birth dated at E13.5 with EdU showed a lower layer bias, while FT labeling of apical progenitors showed no bias. Finally, examination of the morphologies of “biased” E16.5- and P0-labeled astrocytes demonstrated that E16.5-labeled astrocytes exhibit different morphologies in different layers, while P0-labeled astrocytes do not. Differences based on time of birth are also observed in the molecular profiles of E16.5 versus P0-labeled astrocytes. Altogether, these results suggest that the morphological, molecular, and positional diversity of cortical astrocytes is related to their time of birth from ventricular/subventricular zone progenitors.</p>","PeriodicalId":174,"journal":{"name":"Glia","volume":"72 9","pages":"1693-1706"},"PeriodicalIF":5.4000,"publicationDate":"2024-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/glia.24578","citationCount":"0","resultStr":"{\"title\":\"The laminar position, morphology, and gene expression profiles of cortical astrocytes are influenced by time of birth from ventricular/subventricular progenitors\",\"authors\":\"Daniela Lozano Casasbuenas,&nbsp;Ines Kortebi,&nbsp;Charles Gora,&nbsp;Erica Y. Scott,&nbsp;Celeste Gomes,&nbsp;Markley Silva Oliveira Jr,&nbsp;Tanvi Sharma,&nbsp;Emerson Daniele,&nbsp;Arman Olfat,&nbsp;Rachel Gibbs,&nbsp;Scott A. Yuzwa,&nbsp;Emily A. Gilbert,&nbsp;Patrick Küry,&nbsp;Aaron R. Wheeler,&nbsp;Martin Lévesque,&nbsp;Maryam Faiz\",\"doi\":\"10.1002/glia.24578\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Astrocytes that reside in superficial (SL) and deep cortical layers have distinct molecular profiles and morphologies, which may underlie specific functions. Here, we demonstrate that the production of SL and deep layer (DL) astrocyte populations from neural progenitor cells in the mouse is temporally regulated. Lineage tracking following <i>in utero</i> and postnatal electroporation with <i>PiggyBac</i> (<i>PB</i>) EGFP and birth dating with EdU and FlashTag, showed that apical progenitors produce astrocytes during late embryogenesis (E16.5) that are biased to the SL, while postnatally labeled (P0) astrocytes are biased to the DL. In contrast, astrocytes born during the predominantly neurogenic window (E14.5) showed a random distribution in the SL and DL. Of interest, E13.5 astrocytes birth dated at E13.5 with EdU showed a lower layer bias, while FT labeling of apical progenitors showed no bias. Finally, examination of the morphologies of “biased” E16.5- and P0-labeled astrocytes demonstrated that E16.5-labeled astrocytes exhibit different morphologies in different layers, while P0-labeled astrocytes do not. Differences based on time of birth are also observed in the molecular profiles of E16.5 versus P0-labeled astrocytes. Altogether, these results suggest that the morphological, molecular, and positional diversity of cortical astrocytes is related to their time of birth from ventricular/subventricular zone progenitors.</p>\",\"PeriodicalId\":174,\"journal\":{\"name\":\"Glia\",\"volume\":\"72 9\",\"pages\":\"1693-1706\"},\"PeriodicalIF\":5.4000,\"publicationDate\":\"2024-06-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/glia.24578\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Glia\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/glia.24578\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"NEUROSCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Glia","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/glia.24578","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"NEUROSCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

驻留在大脑皮层浅层(SL)和深层的星形胶质细胞具有不同的分子特征和形态,这可能是其特定功能的基础。在这里,我们证明了小鼠神经祖细胞产生的浅层和深层(DL)星形胶质细胞群是受时间调控的。用 PiggyBac (PB) EGFP 在子宫内和出生后进行电穿孔并用 EdU 和 FlashTag 进行出生测序后进行的系谱追踪显示,顶端祖细胞在胚胎发生晚期(E16.5)产生的星形胶质细胞偏向于 SL 层,而出生后标记(P0)的星形胶质细胞偏向于 DL 层。相反,在主要神经源窗口期(E14.5)出生的星形胶质细胞在 SL 和 DL 呈随机分布。值得注意的是,E13.5 出生的星形胶质细胞用 EdU 标记显示偏向下层,而顶端祖细胞的 FT 标记则没有显示偏向。最后,对 E16.5 和 P0 标记的 "偏倚 "星形胶质细胞形态的研究表明,E16.5 标记的星形胶质细胞在不同层表现出不同的形态,而 P0 标记的星形胶质细胞则没有。根据出生时间的不同,E16.5 和 P0 标记的星形胶质细胞的分子特征也有所不同。总之,这些结果表明,大脑皮层星形胶质细胞的形态、分子和位置多样性与它们从室管膜/室管膜下区祖细胞中诞生的时间有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
The laminar position, morphology, and gene expression profiles of cortical astrocytes are influenced by time of birth from ventricular/subventricular progenitors

Astrocytes that reside in superficial (SL) and deep cortical layers have distinct molecular profiles and morphologies, which may underlie specific functions. Here, we demonstrate that the production of SL and deep layer (DL) astrocyte populations from neural progenitor cells in the mouse is temporally regulated. Lineage tracking following in utero and postnatal electroporation with PiggyBac (PB) EGFP and birth dating with EdU and FlashTag, showed that apical progenitors produce astrocytes during late embryogenesis (E16.5) that are biased to the SL, while postnatally labeled (P0) astrocytes are biased to the DL. In contrast, astrocytes born during the predominantly neurogenic window (E14.5) showed a random distribution in the SL and DL. Of interest, E13.5 astrocytes birth dated at E13.5 with EdU showed a lower layer bias, while FT labeling of apical progenitors showed no bias. Finally, examination of the morphologies of “biased” E16.5- and P0-labeled astrocytes demonstrated that E16.5-labeled astrocytes exhibit different morphologies in different layers, while P0-labeled astrocytes do not. Differences based on time of birth are also observed in the molecular profiles of E16.5 versus P0-labeled astrocytes. Altogether, these results suggest that the morphological, molecular, and positional diversity of cortical astrocytes is related to their time of birth from ventricular/subventricular zone progenitors.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Glia
Glia 医学-神经科学
CiteScore
13.10
自引率
4.80%
发文量
162
审稿时长
3-8 weeks
期刊介绍: GLIA is a peer-reviewed journal, which publishes articles dealing with all aspects of glial structure and function. This includes all aspects of glial cell biology in health and disease.
期刊最新文献
Microglia and Astrocytes in Postnatal Neural Circuit Formation. Astrocytic GAT-3 Regulates Synaptic Transmission and Memory Formation in the Dentate Gyrus. All the single cells: Single-cell transcriptomics/epigenomics experimental design and analysis considerations for glial biologists. R-Ras1 and R-Ras2 regulate mature oligodendrocyte subpopulations. Astrocytic NHERF-1 Increases Seizure Susceptibility by Inhibiting Surface Expression of TREK-1.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1