Iliani Patinho , Daniel S. Antonelo , Eduardo F. Delgado , Laura Alessandroni , Júlio C.C. Balieiro , Carmen J. Contreras Castillo , Mohammed Gagaoua
{"title":"深入探索高pH值和正常pH值牛肉蛋白质组:首次深入了解内洛尔牧场饲养公牛胸长肌蛋白质在不同宰后时间的动态变化","authors":"Iliani Patinho , Daniel S. Antonelo , Eduardo F. Delgado , Laura Alessandroni , Júlio C.C. Balieiro , Carmen J. Contreras Castillo , Mohammed Gagaoua","doi":"10.1016/j.meatsci.2024.109557","DOIUrl":null,"url":null,"abstract":"<div><p>This study aimed to evaluate for the first time the temporal dynamic changes in early <em>postmortem</em> proteome of normal and high ultimate pH (pHu) beef samples from the same cattle using a shotgun proteomics approach. Ten selected carcasses classified as normal (pHu < 5.8; <em>n</em> = 5) or high (pHu ≥ 6.2; n = 5) pHu beef from pasture-finished Nellore (<em>Bos taurus indicus</em>) bulls were sampled from <em>Longissimus thoracis</em> muscle at 30 min, 9 h and 44 h <em>postmortem</em> for proteome comparison. The temporal proteomics profiling quantified 863 proteins, from which 251 were differentially abundant (DAPs) between high and normal pHu at 30 min (<em>n</em> = 33), 9 h (<em>n</em> = 181) and 44 h (<em>n</em> = 37). Among the myriad interconnected pathways regulating pH decline during <em>postmortem</em> metabolism, this study revealed the pivotal role of energy metabolism, cellular response to stress, oxidoreductase activity and muscle system process pathways throughout the early <em>postmortem</em>. Twenty-three proteins overlap among <em>postmortem</em> times and may be suggested as candidate biomarkers to the dark-cutting condition development. The study further evidenced for the first time the central role of ribosomal proteins and histones in the first minutes after animal bleeding. Moreover, this study revealed the disparity in the mechanisms underpinning the development of dark-cutting beef condition among <em>postmortem</em> times, emphasizing multiple dynamic changes in the muscle proteome. Therefore, this study revealed important insights regarding the temporal dynamic changes that occur in early <em>postmortem</em> of high and normal muscle pHu beef, proposing specific pathways to determine the biological mechanisms behind dark-cutting determination.</p></div>","PeriodicalId":389,"journal":{"name":"Meat Science","volume":null,"pages":null},"PeriodicalIF":7.1000,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"In-depth exploration of the high and normal pH beef proteome: First insights emphasizing the dynamic protein changes in Longissimus thoracis muscle from pasture-finished Nellore bulls over different postmortem times\",\"authors\":\"Iliani Patinho , Daniel S. Antonelo , Eduardo F. Delgado , Laura Alessandroni , Júlio C.C. Balieiro , Carmen J. Contreras Castillo , Mohammed Gagaoua\",\"doi\":\"10.1016/j.meatsci.2024.109557\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>This study aimed to evaluate for the first time the temporal dynamic changes in early <em>postmortem</em> proteome of normal and high ultimate pH (pHu) beef samples from the same cattle using a shotgun proteomics approach. Ten selected carcasses classified as normal (pHu < 5.8; <em>n</em> = 5) or high (pHu ≥ 6.2; n = 5) pHu beef from pasture-finished Nellore (<em>Bos taurus indicus</em>) bulls were sampled from <em>Longissimus thoracis</em> muscle at 30 min, 9 h and 44 h <em>postmortem</em> for proteome comparison. The temporal proteomics profiling quantified 863 proteins, from which 251 were differentially abundant (DAPs) between high and normal pHu at 30 min (<em>n</em> = 33), 9 h (<em>n</em> = 181) and 44 h (<em>n</em> = 37). Among the myriad interconnected pathways regulating pH decline during <em>postmortem</em> metabolism, this study revealed the pivotal role of energy metabolism, cellular response to stress, oxidoreductase activity and muscle system process pathways throughout the early <em>postmortem</em>. Twenty-three proteins overlap among <em>postmortem</em> times and may be suggested as candidate biomarkers to the dark-cutting condition development. The study further evidenced for the first time the central role of ribosomal proteins and histones in the first minutes after animal bleeding. Moreover, this study revealed the disparity in the mechanisms underpinning the development of dark-cutting beef condition among <em>postmortem</em> times, emphasizing multiple dynamic changes in the muscle proteome. Therefore, this study revealed important insights regarding the temporal dynamic changes that occur in early <em>postmortem</em> of high and normal muscle pHu beef, proposing specific pathways to determine the biological mechanisms behind dark-cutting determination.</p></div>\",\"PeriodicalId\":389,\"journal\":{\"name\":\"Meat Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":7.1000,\"publicationDate\":\"2024-06-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Meat Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0309174024001347\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Meat Science","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0309174024001347","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
In-depth exploration of the high and normal pH beef proteome: First insights emphasizing the dynamic protein changes in Longissimus thoracis muscle from pasture-finished Nellore bulls over different postmortem times
This study aimed to evaluate for the first time the temporal dynamic changes in early postmortem proteome of normal and high ultimate pH (pHu) beef samples from the same cattle using a shotgun proteomics approach. Ten selected carcasses classified as normal (pHu < 5.8; n = 5) or high (pHu ≥ 6.2; n = 5) pHu beef from pasture-finished Nellore (Bos taurus indicus) bulls were sampled from Longissimus thoracis muscle at 30 min, 9 h and 44 h postmortem for proteome comparison. The temporal proteomics profiling quantified 863 proteins, from which 251 were differentially abundant (DAPs) between high and normal pHu at 30 min (n = 33), 9 h (n = 181) and 44 h (n = 37). Among the myriad interconnected pathways regulating pH decline during postmortem metabolism, this study revealed the pivotal role of energy metabolism, cellular response to stress, oxidoreductase activity and muscle system process pathways throughout the early postmortem. Twenty-three proteins overlap among postmortem times and may be suggested as candidate biomarkers to the dark-cutting condition development. The study further evidenced for the first time the central role of ribosomal proteins and histones in the first minutes after animal bleeding. Moreover, this study revealed the disparity in the mechanisms underpinning the development of dark-cutting beef condition among postmortem times, emphasizing multiple dynamic changes in the muscle proteome. Therefore, this study revealed important insights regarding the temporal dynamic changes that occur in early postmortem of high and normal muscle pHu beef, proposing specific pathways to determine the biological mechanisms behind dark-cutting determination.
期刊介绍:
The aim of Meat Science is to serve as a suitable platform for the dissemination of interdisciplinary and international knowledge on all factors influencing the properties of meat. While the journal primarily focuses on the flesh of mammals, contributions related to poultry will be considered if they enhance the overall understanding of the relationship between muscle nature and meat quality post mortem. Additionally, papers on large birds (e.g., emus, ostriches) as well as wild-captured mammals and crocodiles will be welcomed.