Si-Zhe Chen, Jing-Shuai Chen, Xing-Pei Liu, Chang-Jie Mao and Bao-Kang Jin
{"title":"基于 Ru(bpy)32+ 敏化 In2S3 的夹层式光电化学生物传感器,用于检测黄曲霉毒素 B1。","authors":"Si-Zhe Chen, Jing-Shuai Chen, Xing-Pei Liu, Chang-Jie Mao and Bao-Kang Jin","doi":"10.1039/D4AN00612G","DOIUrl":null,"url":null,"abstract":"<p >Aflatoxin B<small><sub>1</sub></small> (AFB<small><sub>1</sub></small>), classified as a class I carcinogen, is a widespread mycotoxin that poses a serious threat to public health and economic development, and the food safety problems caused by AFB<small><sub>1</sub></small> have aroused worldwide concern. The development of accurate and sensitive methods for the detection of AFB<small><sub>1</sub></small> is significant for food safety monitoring. In this work, a sandwich-type photoelectrochemical (PEC) biosensor for AFB<small><sub>1</sub></small> detection was constructed on the basis of an aptamer–antibody structure. A good photocurrent response was obtained due to the sensitization of In<small><sub>2</sub></small>S<small><sub>3</sub></small> by Ru(bpy)<small><sub>3</sub></small><small><sup>2+</sup></small>. In addition, this sandwich-type sensor constructed by modification with the antibody, target detector, and aptamer layer by layer attenuated the migration hindering effect of photogenerated carriers caused by the double antibody structure. The aptamer and antibody synergistically recognized and captured the target analyte, resulting in more reliable PEC response signals. CdSe@CdS QDs-Apt were modified as a signal-off probe onto the sensor platform to quantitatively detect AFB<small><sub>1</sub></small> with a “signal-off” response, which enhanced the sensitivity of the sensor. The PEC biosensor showed a linear response range from 10<small><sup>−12</sup></small> to 10<small><sup>−6</sup></small> g mL<small><sup>−1</sup></small> with a detection limit of 0.023 pg mL<small><sup>−1</sup></small>, providing a feasible approach for the quantitative detection of AFB<small><sub>1</sub></small> in food samples.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":null,"pages":null},"PeriodicalIF":3.6000,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A sandwich-type photoelectrochemical biosensor based on Ru(bpy)32+ sensitized In2S3 for aflatoxin B1 detection†\",\"authors\":\"Si-Zhe Chen, Jing-Shuai Chen, Xing-Pei Liu, Chang-Jie Mao and Bao-Kang Jin\",\"doi\":\"10.1039/D4AN00612G\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Aflatoxin B<small><sub>1</sub></small> (AFB<small><sub>1</sub></small>), classified as a class I carcinogen, is a widespread mycotoxin that poses a serious threat to public health and economic development, and the food safety problems caused by AFB<small><sub>1</sub></small> have aroused worldwide concern. The development of accurate and sensitive methods for the detection of AFB<small><sub>1</sub></small> is significant for food safety monitoring. In this work, a sandwich-type photoelectrochemical (PEC) biosensor for AFB<small><sub>1</sub></small> detection was constructed on the basis of an aptamer–antibody structure. A good photocurrent response was obtained due to the sensitization of In<small><sub>2</sub></small>S<small><sub>3</sub></small> by Ru(bpy)<small><sub>3</sub></small><small><sup>2+</sup></small>. In addition, this sandwich-type sensor constructed by modification with the antibody, target detector, and aptamer layer by layer attenuated the migration hindering effect of photogenerated carriers caused by the double antibody structure. The aptamer and antibody synergistically recognized and captured the target analyte, resulting in more reliable PEC response signals. CdSe@CdS QDs-Apt were modified as a signal-off probe onto the sensor platform to quantitatively detect AFB<small><sub>1</sub></small> with a “signal-off” response, which enhanced the sensitivity of the sensor. The PEC biosensor showed a linear response range from 10<small><sup>−12</sup></small> to 10<small><sup>−6</sup></small> g mL<small><sup>−1</sup></small> with a detection limit of 0.023 pg mL<small><sup>−1</sup></small>, providing a feasible approach for the quantitative detection of AFB<small><sub>1</sub></small> in food samples.</p>\",\"PeriodicalId\":63,\"journal\":{\"name\":\"Analyst\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.6000,\"publicationDate\":\"2024-06-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analyst\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://pubs.rsc.org/en/content/articlelanding/2024/an/d4an00612g\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analyst","FirstCategoryId":"92","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2024/an/d4an00612g","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
A sandwich-type photoelectrochemical biosensor based on Ru(bpy)32+ sensitized In2S3 for aflatoxin B1 detection†
Aflatoxin B1 (AFB1), classified as a class I carcinogen, is a widespread mycotoxin that poses a serious threat to public health and economic development, and the food safety problems caused by AFB1 have aroused worldwide concern. The development of accurate and sensitive methods for the detection of AFB1 is significant for food safety monitoring. In this work, a sandwich-type photoelectrochemical (PEC) biosensor for AFB1 detection was constructed on the basis of an aptamer–antibody structure. A good photocurrent response was obtained due to the sensitization of In2S3 by Ru(bpy)32+. In addition, this sandwich-type sensor constructed by modification with the antibody, target detector, and aptamer layer by layer attenuated the migration hindering effect of photogenerated carriers caused by the double antibody structure. The aptamer and antibody synergistically recognized and captured the target analyte, resulting in more reliable PEC response signals. CdSe@CdS QDs-Apt were modified as a signal-off probe onto the sensor platform to quantitatively detect AFB1 with a “signal-off” response, which enhanced the sensitivity of the sensor. The PEC biosensor showed a linear response range from 10−12 to 10−6 g mL−1 with a detection limit of 0.023 pg mL−1, providing a feasible approach for the quantitative detection of AFB1 in food samples.