NF-kappaB p52 亚基二聚化结构域的骨架三重共振分配。

IF 0.8 4区 生物学 Q4 BIOPHYSICS Biomolecular NMR Assignments Pub Date : 2024-06-10 DOI:10.1007/s12104-024-10179-1
Sunirmala Sahoo, Nitin Dhaka, Sulakshana P. Mukherjee
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引用次数: 0

摘要

NF-kappaB 是一个可诱导的转录因子家族,在脊椎动物的免疫反应中发挥着重要作用。该家族的五个成员以不同的组合形式发挥二聚体的功能。尽管所有家族成员都能识别并结合到相似的 DNA 元件上以调节其目标基因的转录,但二聚体的组成会导致不同的转录结果。在这里,我们报告了 NF-kB 家族 p52 亚基 24.2 kDa 同源二聚体的骨架共振分配。NF-kB 的 p52 亚基是非经典 NF-kB 通路中的一个重要角色,它的失调在导致各种炎症性疾病和癌症的免疫反应中显示出有害影响。虽然本研究中利用骨架化学位移预测的 β 链与现有晶体结构基本吻合,但在我们的研究结果中却没有观察到晶体结构中存在的螺旋转折。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Backbone triple resonance assignments of the dimerization domain of NF-kappaB p52 subunit

NF-kappaB is a family of inducible transcription factors playing an important role in immune response in vertebrates. All the five members of the family function as dimers in various combinations. Though all the family members recognize and bind to similar DNA elements to regulate the transcription of its target genes, the dimer composition can lead to differential transcriptional outcomes. Here we report the backbone resonance assignment of the 24.2 kDa homodimer of p52 subunit of the NF-kB family. The p52 subunit of NF-kB is a crucial player in the non-canonical NF-kB pathway and its dysregulation has shown detrimental effects in immune response leading to various inflammatory diseases and cancers. While the β-strands predicted using the backbone chemical shifts in this study largely conform with the available crystal structure, the helical turns present in the crystal structure are not observed in our results.

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来源期刊
Biomolecular NMR Assignments
Biomolecular NMR Assignments 生物-光谱学
CiteScore
1.70
自引率
11.10%
发文量
59
审稿时长
6-12 weeks
期刊介绍: Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties. Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.
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