作为种植体周围健康或疾病的生物标志物,种植体周围龈沟液中的 aMMP-8 和 azurocidin 具有诊断价值。

IF 1.7 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Clinical and Experimental Dental Research Pub Date : 2024-06-09 DOI:10.1002/cre2.883
Vithleem Xanthopoulou, Ismo T. Räisänen, Timo Sorsa, Dimitrios Tortopidis, Dimitra Sakellari
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引用次数: 0

摘要

研究目的本研究的目的是探讨通过定量床旁侧流免疫测定(PoC)检测种植体周围龈沟液(PISF)中的活性基质金属蛋白酶-8(aMMP-8)和氮芥作为生物标志物检测种植体周围疾病存在与否的有效性:背景:目前的研究表明,促炎细胞因子和细胞外基质降解酶可能对诊断和预测种植体周围疾病的发生和发展有价值,但还需要更多的数据:方法:招募了 80 名种植体患者。收集 PISF 样本,并用 ELISA 对 aMMP-8(椅旁)和azurocidin 进行定量分析。取样后记录放射学评估和临床指标(探诊深度、探诊附着水平、探诊出血和牙菌斑)。采用 Kruskal-Wallis 检验和配对后 Dunn-Bonferroni 检验将 aMMP-8 和 Azurocidin 水平与临床指标联系起来。通过接收者运算曲线分析法分析了aMMP-8(纳克/毫升)和氮芥苷的诊断能力。计算了曲线下面积(AUC),并使用斯皮尔曼rho和判定系数(R2)计算了aMMP-8、氮芥和牙周参数之间的相关性:结果:在健康种植体、粘膜炎种植体和种植体周围炎种植体之间,aMMP-8 水平有统计学意义的差异(分别为 13.65 ± 7.18、32.33 ± 21.20 和 73.07 ± 43.93 ng/mL),而氮杂络氨酸水平无统计学意义的差异(Kruskall-Wallis 检验 p 结论:综上所述,本数据表明 aMMP-8 PoC 侧流免疫测定是一种有益的辅助诊断定量工具,可用于实时筛查种植体周围疾病。
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Diagnostic value of aMMP-8 and azurocidin in peri-implant sulcular fluid as biomarkers of peri-implant health or disease

Objective

The objective of this study was to investigate the effectiveness of testing for active matrix metalloproteinase-8 (aMMP-8) by a quantitative point-of-care (PoC), chairside lateral flow immunotest and azurocidin, in the peri-implant sulcular fluid (PISF), as biomarkers for the presence or absence of peri-implant diseases.

Background

Current research indicates that proinflammatory cytokines and extracellular matrix-degrading enzymes may be of value to diagnose and predict peri-implant disease initiation and progression, but more data are needed.

Methods

Eighty patients with implants were recruited. PISF samples were collected and quantitatively analyzed for aMMP-8 (chairside) and azurocidin with ELISA. Radiographic assessments and clinical indices (probing depth, probing attachment level, bleeding on probing, and plaque) were recorded after sampling. Kruskal-Wallis test and pairwise post hoc Dunn-Bonferroni test were used to relate aMMP-8 levels and azurocidin levels to clinical parameters. The diagnostic ability of aMMP-8 (ng/mL) and azurocidin was analyzed by receiver operator curve analysis. Area under the curve (AUC) was calculated and the Spearman's rho, and the coefficient of determination (R2) were used to calculate the correlations between aMMP-8, azurocidin, and periodontal parameters.

Results

Statistically significant differences were observed for aMMP-8 levels but not for azurocidin between healthy implants, implants with mucositis, and those with peri-implantitis (13.65 ± 7.18, 32.33 ± 21.20, and 73.07 ± 43.93 ng/mL, respectively), (Kruskall–Wallis test p < .05). The aMMP-8 test with a threshold of 20 ng/mL has a sensitivity of 71.7% and a specificity of 77.8% to identify peri-implantitis and healthy implants, respectively. AUC was found to be 0.814, and the accuracy of the method reaches 73.8%. Above a cutoff value of 33.7 ng/mL of aMMP-8, the accuracy of the test to detect peri-implantitis reaches 77.5% in relation to 62.5% of BoP from the same site.

Conclusion

Taken collectively, present data indicate that the aMMP-8 PoC lateral flow immunotest can be a beneficial, adjunctive diagnostic quantitative tool for real-time screening for peri-implant diseases.

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来源期刊
Clinical and Experimental Dental Research
Clinical and Experimental Dental Research DENTISTRY, ORAL SURGERY & MEDICINE-
CiteScore
3.30
自引率
5.60%
发文量
165
审稿时长
26 weeks
期刊介绍: Clinical and Experimental Dental Research aims to provide open access peer-reviewed publications of high scientific quality representing original clinical, diagnostic or experimental work within all disciplines and fields of oral medicine and dentistry. The scope of Clinical and Experimental Dental Research comprises original research material on the anatomy, physiology and pathology of oro-facial, oro-pharyngeal and maxillofacial tissues, and functions and dysfunctions within the stomatognathic system, and the epidemiology, aetiology, prevention, diagnosis, prognosis and therapy of diseases and conditions that have an effect on the homeostasis of the mouth, jaws, and closely associated structures, as well as the healing and regeneration and the clinical aspects of replacement of hard and soft tissues with biomaterials, and the rehabilitation of stomatognathic functions. Studies that bring new knowledge on how to advance health on the individual or public health levels, including interactions between oral and general health and ill-health are welcome.
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