Butyrate is one of major metabolites of periodontitis-associated bacteria and often detected in periodontal pockets. Butyrate has been considered to affect human gingival fibroblasts (HGFs); however, there was no information on its long-term effect as occurs in periodontitis. Therefore, this study aimed to evaluate the time-dependent effects of butyrate on HGFs.
Material and Methods
The effects of butyrate on HGF proliferation, apoptosis, cell morphology, glucose metabolic activity, butyrate metabolic activity, and cell migration ability were evaluated by cell counting, DNA electrophoresis, cell staining, pH-stat system, HPLC, and scratch test, respectively.
Results
HGF proliferation was temporarily inhibited by 5–10 mM butyrate (p < 0.05); however, it resumed at 24 h with morphological changes from spindle to slightly widened (p < 0.05). HGFs cultured with 10 mM butyrate for 12–24 h shifted the glucose metabolic pathway from oxidative phosphorylation to glycolysis (p < 0.05), and increased butyrate consumption, which returned to control levels over 24 h. HGF migration ability tended to decrease at 72 h.
Conclusions
HGF cell proliferation and glucose/butyrate metabolism were temporarily inhibited by butyrate and then recovered in a time-dependent manner, accompanied by changes in cell morphology. These time-dependent effects may help to understand the role of butyrate in the pathology of periodontitis.
{"title":"Time-Dependent Changes in Effects of Butyrate on Human Gingival Fibroblasts","authors":"Haruki Otani, Jumpei Washio, Aoi Kunitomi, Satoko Sato, Yuki Abiko, Shiori Sasaki, Kazumasa Ohashi, Satoru Yamada, Nobuhiro Takahashi","doi":"10.1002/cre2.70120","DOIUrl":"https://doi.org/10.1002/cre2.70120","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Butyrate is one of major metabolites of periodontitis-associated bacteria and often detected in periodontal pockets. Butyrate has been considered to affect human gingival fibroblasts (HGFs); however, there was no information on its long-term effect as occurs in periodontitis. Therefore, this study aimed to evaluate the time-dependent effects of butyrate on HGFs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>The effects of butyrate on HGF proliferation, apoptosis, cell morphology, glucose metabolic activity, butyrate metabolic activity, and cell migration ability were evaluated by cell counting, DNA electrophoresis, cell staining, pH-stat system, HPLC, and scratch test, respectively.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>HGF proliferation was temporarily inhibited by 5–10 mM butyrate (<i>p</i> < 0.05); however, it resumed at 24 h with morphological changes from spindle to slightly widened (<i>p</i> < 0.05). HGFs cultured with 10 mM butyrate for 12–24 h shifted the glucose metabolic pathway from oxidative phosphorylation to glycolysis (<i>p</i> < 0.05), and increased butyrate consumption, which returned to control levels over 24 h. HGF migration ability tended to decrease at 72 h.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>HGF cell proliferation and glucose/butyrate metabolism were temporarily inhibited by butyrate and then recovered in a time-dependent manner, accompanied by changes in cell morphology. These time-dependent effects may help to understand the role of butyrate in the pathology of periodontitis.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 3","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70120","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143871705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zahra Hashemzade, Mohammad Alihemmati, Seyed Mohammad Reza Hakimaneh, Sayed Shojaedin Shayegh, Mohammad Amin Bafandeh, Zahra Mohammadi
Objectives
Manufacturing temporary restorations is part of the treatment process in fixed prostheses, which is accomplished by different methods. The aim of this study is to compare the color stability and surface roughness of provisional crowns made by 3D printing, conventional and milling methods.
Materials and Methods
In this semi-experimental study, 60 provisional crowns were created by conventional, milling, and 3D printing methods (20 samples for each method). Half of the samples in each group were treated with BisCover surface sealant after construction. The surface roughness was checked using a laser profilometer. To determine the color stability, an evaluation was done using a spectrophotometer on the first day and the second and fourth weeks after exposure to the tea solution, and ∆E was calculated using L*a*b* values. Statistical analysis was performed at a significance level of 0.05.
Results
The surface roughness in the conventional group was significantly higher than in the milling group (p < 0.05). The surface-treated samples had less surface roughness and more color stability than other samples (p < 0.05). ∆E in the 3D printing group was higher than other groups in all time intervals (p < 0.05).
Conclusion
The milling method can be considered the best method of making provisional crowns in terms of color stability and surface smoothness. Also, the use of sealing materials can have a significant effect on improving color stability and surface smoothness in provisional crowns made by any method.
{"title":"Comparison of Color Stability and Surface Roughness of Interim Crowns Fabricated by Conventional, Milling and 3D Printing Methods","authors":"Zahra Hashemzade, Mohammad Alihemmati, Seyed Mohammad Reza Hakimaneh, Sayed Shojaedin Shayegh, Mohammad Amin Bafandeh, Zahra Mohammadi","doi":"10.1002/cre2.70119","DOIUrl":"https://doi.org/10.1002/cre2.70119","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Manufacturing temporary restorations is part of the treatment process in fixed prostheses, which is accomplished by different methods. The aim of this study is to compare the color stability and surface roughness of provisional crowns made by 3D printing, conventional and milling methods.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>In this semi-experimental study, 60 provisional crowns were created by conventional, milling, and 3D printing methods (20 samples for each method). Half of the samples in each group were treated with BisCover surface sealant after construction. The surface roughness was checked using a laser profilometer. To determine the color stability, an evaluation was done using a spectrophotometer on the first day and the second and fourth weeks after exposure to the tea solution, and ∆<i>E</i> was calculated using L*a*b* values. Statistical analysis was performed at a significance level of 0.05.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The surface roughness in the conventional group was significantly higher than in the milling group (<i>p</i> < 0.05). The surface-treated samples had less surface roughness and more color stability than other samples (<i>p</i> < 0.05). ∆E in the 3D printing group was higher than other groups in all time intervals (<i>p</i> < 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The milling method can be considered the best method of making provisional crowns in terms of color stability and surface smoothness. Also, the use of sealing materials can have a significant effect on improving color stability and surface smoothness in provisional crowns made by any method.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 2","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70119","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Achieving optimal esthetics in dental restorations remains challenging, particularly with zirconia crowns, which, despite their durability, often exhibit less translucency than natural teeth. This study aimed to evaluate how varying thicknesses of monolithic zirconia crowns affect their translucency.
Materials and Methods
In this experimental laboratory study, an initial model (a brass die) was scanned, and two dies, one black (9005 RAL), and one white (9010 RAL), were created from Resin according to the scanned file. Next, 30 zirconia crowns (ZrO2, Arum, High Translucent) were fabricated in three thicknesses: 0.5, 0.8, and 1.5 mm. The color and translucency of the samples were assessed under standard lighting conditions (D65) using a spectroradiometer, and the color parameters L*, b*, and a* were recorded. The translucency of each sample was measured using the CIEDE2000 and CIE-Lab formulae. Data were analyzed using one-way ANOVA and the Tamhane test.
Results
Statistically significant changes in the color and translucency of the samples were observed with varying thicknesses (p < 0.001). Specifically, the translucency parameter (TP), L*, and b* values decreased as the thickness increased, whereas a* values remained relatively stable. The average TP values for CIE-Lab ranged from 11.51 at 0.5 mm to 6.54 at 1.5 mm, and for CIEDE2000, they ranged from 8.19 at 0.5 mm to 4.82 at 1.5 mm.
Conclusion
Within the limitations of this in vitro study, it can be concluded that reducing the thickness of monolithic zirconia restorations increases their translucency. Thinner zirconia restorations demonstrate a higher TP, which may offer improved esthetic integration in clinical applications.
{"title":"Comparative Analysis of Translucency in Different Thicknesses of Monolithic Zirconia Crowns","authors":"Azita Mazaheri, Ezatollah Jalalian, Arash Zarbakhsh, Mahshad Mazidabadi Farahani, Maryam Sayyari","doi":"10.1002/cre2.70132","DOIUrl":"https://doi.org/10.1002/cre2.70132","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Achieving optimal esthetics in dental restorations remains challenging, particularly with zirconia crowns, which, despite their durability, often exhibit less translucency than natural teeth. This study aimed to evaluate how varying thicknesses of monolithic zirconia crowns affect their translucency.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>In this experimental laboratory study, an initial model (a brass die) was scanned, and two dies, one black (9005 RAL), and one white (9010 RAL), were created from Resin according to the scanned file. Next, 30 zirconia crowns (ZrO<sub>2</sub>, Arum, High Translucent) were fabricated in three thicknesses: 0.5, 0.8, and 1.5 mm. The color and translucency of the samples were assessed under standard lighting conditions (D65) using a spectroradiometer, and the color parameters <i>L</i>*, <i>b</i>*, and <i>a</i>* were recorded. The translucency of each sample was measured using the CIEDE2000 and CIE-Lab formulae. Data were analyzed using one-way ANOVA and the Tamhane test.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Statistically significant changes in the color and translucency of the samples were observed with varying thicknesses (<i>p</i> < 0.001). Specifically, the translucency parameter (TP), <i>L</i>*, and <i>b</i>* values decreased as the thickness increased, whereas <i>a</i>* values remained relatively stable. The average TP values for CIE-Lab ranged from 11.51 at 0.5 mm to 6.54 at 1.5 mm, and for CIEDE2000, they ranged from 8.19 at 0.5 mm to 4.82 at 1.5 mm.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Within the limitations of this in vitro study, it can be concluded that reducing the thickness of monolithic zirconia restorations increases their translucency. Thinner zirconia restorations demonstrate a higher TP, which may offer improved esthetic integration in clinical applications.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 2","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70132","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ali Nozari, Farnaz Haji Abbas Oghli, Fatemeh Parvizi, Zahra Jowkar, Maryam Pakniyat Jahromi, Seyed Ahmadreza Hamidi
Objectives
This study aimed to assess the impact of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) and proanthocyanidin (PA) on the microshear bond strength (μSBS) of universal adhesives to caries-affected dentin (CAD) in primary teeth.
Materials and Methods
160 human primary second molars with occlusal caries were utilized, with CAD-exposed dentin surfaces. The teeth were categorized into four groups based on CAD pretreatment: no pretreatment, CPP-ACP for 3 min, PA for 1 min, and PA for 1 min followed by CPP-ACP for 3 min. Each group subdivided into four based on adhesive system (Gluma Bond Universal or All-Bond Universal) and application mode (etch and rinse; E&R or self-etch; SE). Following composite resin restoration, μSBS measurements were taken after 24 h of water storage.
Results
PA pretreatment showed the highest μSBS compared to controls and other methods (p < 0.001). Conversely, CAD pretreatment with CPP-ACP + PA led to lower μSBS than the control (p = 0.009). Universal adhesive choice significantly influenced μSBS (p < 0.001), with Gluma Bond Universal outperforming All-Bond Universal (p < 0.001). The E&R method demonstrated superior bond strength over SE (p < 0.001).
Conclusion
CAD pretreatment, particularly with PA, significantly impacted bond strength, with Gluma Bond Universal and the E&R method proving optimal for enhancing μSBS to CAD. These findings offer valuable insights for refining adhesive protocols in pediatric dentistry, potentially improving clinical outcomes in restorative procedures.
{"title":"Impact of Casein Phosphopeptide Amorphous Calcium Phosphate and Proanthocyanidin on Bond Strength of Universal Adhesives to Caries-Affected Dentin in Primary Teeth: An In Vitro Study","authors":"Ali Nozari, Farnaz Haji Abbas Oghli, Fatemeh Parvizi, Zahra Jowkar, Maryam Pakniyat Jahromi, Seyed Ahmadreza Hamidi","doi":"10.1002/cre2.70131","DOIUrl":"https://doi.org/10.1002/cre2.70131","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>This study aimed to assess the impact of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) and proanthocyanidin (PA) on the microshear bond strength (μSBS) of universal adhesives to caries-affected dentin (CAD) in primary teeth.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>160 human primary second molars with occlusal caries were utilized, with CAD-exposed dentin surfaces. The teeth were categorized into four groups based on CAD pretreatment: no pretreatment, CPP-ACP for 3 min, PA for 1 min, and PA for 1 min followed by CPP-ACP for 3 min. Each group subdivided into four based on adhesive system (Gluma Bond Universal or All-Bond Universal) and application mode (etch and rinse; E&R or self-etch; SE). Following composite resin restoration, μSBS measurements were taken after 24 h of water storage.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>PA pretreatment showed the highest μSBS compared to controls and other methods (<i>p</i> < 0.001). Conversely, CAD pretreatment with CPP-ACP + PA led to lower μSBS than the control (<i>p</i> = 0.009). Universal adhesive choice significantly influenced μSBS (<i>p</i> < 0.001), with Gluma Bond Universal outperforming All-Bond Universal (<i>p</i> < 0.001). The E&R method demonstrated superior bond strength over SE (<i>p</i> < 0.001).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>CAD pretreatment, particularly with PA, significantly impacted bond strength, with Gluma Bond Universal and the E&R method proving optimal for enhancing μSBS to CAD. These findings offer valuable insights for refining adhesive protocols in pediatric dentistry, potentially improving clinical outcomes in restorative procedures.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 2","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70131","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Linda J. Dula, Kujtim Sh. Shala, David Stubljar, Andrej Starc, Shera Kosumi
Objectives
This study aimed to evaluate and compare the masticatory efficiency of removable partial dentures (RPDs) retained with clasps versus those retained with attachments.
Material and Methods
A total of 107 patients fitted with 138 RPDs participated in the study; 87 RPDs (63.0%) were clasp-retained, and 51 RPDs (37.0%) were attachment-retained. Subjects chewed 5.0 g of peanuts for 30 s, and masticatory efficiency was measured using a spectrophotometer at an absorption rate of 590 µm. Measurements were taken before insertion, immediately after, and 1 and 3 months post-insertion of RPDs.
Results
The analysis revealed progressive masticatory efficiency improvements for both clasp-retained and attachment-retained RPDs over time, with significant enhancements observed at the 3-month post-insertion mark. Initially, clasp-retained RPDs showed slightly higher efficiency than attachment-retained RPDs; however, attachment-retained RPDs demonstrated superior efficiency after 3 months (p = 0.001). Consistent improvements were noted across different denture support types, with Triangular and Quadrangular supports showing the most notable gains by 3 months (p ≤ 0.006). GLMM analysis underscored the significant impact of time on masticatory efficiency (F(3, 511) = 4.926, p = 0.002), with no significant effects attributed to RPD type or support type alone, nor any significant interaction effects, indicating a universal improvement in masticatory function over time regardless of RPD design.
Conclusions
RPD insertion significantly improves masticatory efficiency, particularly evident 3 months post-insertion, with attachment-retained RPDs outperforming clasp-retained types. Improvements in masticatory function over time are consistent across all RPD designs, unaffected by denture type or support structure. This emphasizes the role of denture design in both immediate adaptation and long-term treatment success, suggesting that time significantly contributes to enhanced masticatory efficiency regardless of RPD design, highlighting the importance of tailored prosthetic rehabilitation.
{"title":"Comparison of Increase in Masticatory Efficiency Between Removable Partial Dentures Retained With Clasps and Retained With Attachments","authors":"Linda J. Dula, Kujtim Sh. Shala, David Stubljar, Andrej Starc, Shera Kosumi","doi":"10.1002/cre2.70130","DOIUrl":"https://doi.org/10.1002/cre2.70130","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>This study aimed to evaluate and compare the masticatory efficiency of removable partial dentures (RPDs) retained with clasps versus those retained with attachments.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>A total of 107 patients fitted with 138 RPDs participated in the study; 87 RPDs (63.0%) were clasp-retained, and 51 RPDs (37.0%) were attachment-retained. Subjects chewed 5.0 g of peanuts for 30 s, and masticatory efficiency was measured using a spectrophotometer at an absorption rate of 590 µm. Measurements were taken before insertion, immediately after, and 1 and 3 months post-insertion of RPDs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The analysis revealed progressive masticatory efficiency improvements for both clasp-retained and attachment-retained RPDs over time, with significant enhancements observed at the 3-month post-insertion mark. Initially, clasp-retained RPDs showed slightly higher efficiency than attachment-retained RPDs; however, attachment-retained RPDs demonstrated superior efficiency after 3 months (<i>p</i> = 0.001). Consistent improvements were noted across different denture support types, with Triangular and Quadrangular supports showing the most notable gains by 3 months (<i>p</i> ≤ 0.006). GLMM analysis underscored the significant impact of time on masticatory efficiency (<i>F</i>(3, 511) = 4.926, <i>p</i> = 0.002), with no significant effects attributed to RPD type or support type alone, nor any significant interaction effects, indicating a universal improvement in masticatory function over time regardless of RPD design.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>RPD insertion significantly improves masticatory efficiency, particularly evident 3 months post-insertion, with attachment-retained RPDs outperforming clasp-retained types. Improvements in masticatory function over time are consistent across all RPD designs, unaffected by denture type or support structure. This emphasizes the role of denture design in both immediate adaptation and long-term treatment success, suggesting that time significantly contributes to enhanced masticatory efficiency regardless of RPD design, highlighting the importance of tailored prosthetic rehabilitation.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 2","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70130","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shabnam Ajami, Seyyed Hadi Hosseini, Neda Babanouri, Zahra Hashemi
Objectives
This study aimed to compare the analgesic efficacy of Bromelain, Ibuprofen, and Bromelain plus turmeric for pain control after orthodontic separator placement.
Material and Methods
This triple-blind randomized clinical trial included 135 patients over the age of 15 who required orthodontic treatment. The patients were randomly assigned to three groups: 400 mg Ibuprofen (Group A), 200 mg Bromelain (ANAHEAL; Group B), and 150 mg Bromelain + 300 mg Turmeric (ANAHEAL PLUS; Group C). The patients took one tablet immediately after the separator was placed. Four separators were placed at the mesial and distal regions of both maxillary first molars. The degree of pain and discomfort was measured using a visual analog scale (VAS) at the following time points: Immediately after the separator placement (T0), 2 h posttreatment (T1), 6 h posttreatment (T2), 24 h posttreatment (T3), and 48 h after separator placement (T4). The data were analyzed using SPSS (version 15.0). Different statistical tests including ANOVA, Tukey's test, and paired t-test were utilized. p < 0.05 was considered statistically significant.
Results
Pain scores at 2 h (T1) and 6 h (T2) after separator placement differed significantly among the three groups (p = 0.006 and p = 0.025, respectively), with the Ibuprofen group experiencing significantly higher pain levels than the Bromelain and Bromelain Plus Turmeric groups. At 48 h (T4), a significant difference was observed only in chewing function (p = 0.024), where patients in the Bromelain group reported higher pain scores than those in the Ibuprofen group. Sex had no significant effect on pain perception (P < 0.05).
Conclusions
Bromelain with or without turmeric might be a useful alternative to Ibuprofen for pain control following orthodontic separator placement.
{"title":"Analgesic Efficacy of Bromelain and Bromelain Plus Turmeric for Pain Control After Orthodontic Separator Placement: A Triple-Blind Randomized Clinical Trial","authors":"Shabnam Ajami, Seyyed Hadi Hosseini, Neda Babanouri, Zahra Hashemi","doi":"10.1002/cre2.70124","DOIUrl":"https://doi.org/10.1002/cre2.70124","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>This study aimed to compare the analgesic efficacy of Bromelain, Ibuprofen, and Bromelain plus turmeric for pain control after orthodontic separator placement.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>This triple-blind randomized clinical trial included 135 patients over the age of 15 who required orthodontic treatment. The patients were randomly assigned to three groups: 400 mg Ibuprofen (Group A), 200 mg Bromelain (ANAHEAL; Group B), and 150 mg Bromelain + 300 mg Turmeric (ANAHEAL PLUS; Group C). The patients took one tablet immediately after the separator was placed. Four separators were placed at the mesial and distal regions of both maxillary first molars. The degree of pain and discomfort was measured using a visual analog scale (VAS) at the following time points: Immediately after the separator placement (T<sub>0</sub>), 2 h posttreatment (T<sub>1</sub>), 6 h posttreatment (T<sub>2</sub>), 24 h posttreatment (T<sub>3</sub>), and 48 h after separator placement (T<sub>4</sub>). The data were analyzed using SPSS (version 15.0). Different statistical tests including ANOVA, Tukey's test, and paired <i>t</i>-test were utilized. <i>p</i> < 0.05 was considered statistically significant.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Pain scores at 2 h (T1) and 6 h (T2) after separator placement differed significantly among the three groups (<i>p</i> = 0.006 and <i>p</i> = 0.025, respectively), with the Ibuprofen group experiencing significantly higher pain levels than the Bromelain and Bromelain Plus Turmeric groups. At 48 h (T4), a significant difference was observed only in chewing function (<i>p</i> = 0.024), where patients in the Bromelain group reported higher pain scores than those in the Ibuprofen group. Sex had no significant effect on pain perception (<i>P</i> < <i>0.05</i>).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Bromelain with or without turmeric might be a useful alternative to Ibuprofen for pain control following orthodontic separator placement.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 2","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70124","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ioanna Politi, Bahman Honari, Lewis Winning, Ioannis Polyzois
Objectives
To compare the implant stability quotient values (ISQ) of implants placed using implant-specific drills (CD) and osseodensification drills (OD) at three different time points and to determine the effect of both drilling techniques on marginal bone levels.
Material and Methods
38 subjects were recruited and ISQ values and radiographic marginal bone levels were recorded after surgery (T1), and also at 3 (T2) and 4–5 months (T3). Clinical and radiographic marginal bone levels were also recorded.
Results
At T1, mean ISQ values ranged from 65.5 to 81 for the CD group and 29 to 80 for the OD group. For the CD group, ISQ values were 72.20 ± 2.6 (95% CI) at T1, 75.0 ± 2.0 at T2, and 74.8 ± 2.3 at T3. The corresponding ISQ values for the OD group were 68.1 ± 5.6, 71.9 ± 1.6, and 72.2 ± 2.4, respectively. Implants placed using CD drills showed greater stability at 3 months but not at placement or at 4–5 months. No statistically significant differences were identified regarding marginal bone levels between the two groups.
Conclusions
There was a notable increase in implant stability over time for both treatment modalities. At T2, implants inserted into osteotomies made with standard drills exhibited significantly greater stability compared to those placed using OD drills. However, the clinical relevance of this difference is questionable, as it was not observed at T3. Marginal bone levels were comparable for both groups over all time points.
Trial Registration
ClinicalTrials.gov identifier: NCT05376020
{"title":"The Effect of Osseodensification on Implant Stability and Marginal Bone Levels: A Randomized Control Clinical Trial","authors":"Ioanna Politi, Bahman Honari, Lewis Winning, Ioannis Polyzois","doi":"10.1002/cre2.70126","DOIUrl":"https://doi.org/10.1002/cre2.70126","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>To compare the implant stability quotient values (ISQ) of implants placed using implant-specific drills (CD) and osseodensification drills (OD) at three different time points and to determine the effect of both drilling techniques on marginal bone levels.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>38 subjects were recruited and ISQ values and radiographic marginal bone levels were recorded after surgery (T1), and also at 3 (T2) and 4–5 months (T3). Clinical and radiographic marginal bone levels were also recorded.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>At T1, mean ISQ values ranged from 65.5 to 81 for the CD group and 29 to 80 for the OD group. For the CD group, ISQ values were 72.20 ± 2.6 (95% CI) at T1, 75.0 ± 2.0 at T2, and 74.8 ± 2.3 at T3. The corresponding ISQ values for the OD group were 68.1 ± 5.6, 71.9 ± 1.6, and 72.2 ± 2.4, respectively. Implants placed using CD drills showed greater stability at 3 months but not at placement or at 4–5 months. No statistically significant differences were identified regarding marginal bone levels between the two groups.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>There was a notable increase in implant stability over time for both treatment modalities. At T2, implants inserted into osteotomies made with standard drills exhibited significantly greater stability compared to those placed using OD drills. However, the clinical relevance of this difference is questionable, as it was not observed at T3. Marginal bone levels were comparable for both groups over all time points.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Trial Registration</h3>\u0000 \u0000 <p>ClinicalTrials.gov identifier: NCT05376020</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70126","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143809470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
David, S., Hüfner, H., Rauch, N., Kerberger, R., Drescher, D., Brunello, G., and Becker B. “Impact of Autoclaving on the Dimensional Stability of 3D-Printed Guides for Orthodontic Mini-Implant Insertion—An In Vitro Study.” Clinical and Experimental Dental Research 11, 2025: e70111.
On page 1, the text “Samuel David and Mira Hüfner contributed equally to this article.” is incorrect and should not have appeared. Samuel David is the first author and Mira Hüfner is the second author, with no shared first authorship.
We apologize for this error.
{"title":"Correction to “Impact of Autoclaving on the Dimensional Stability of 3D-Printed Guides for Orthodontic Mini-Implant Insertion—An In Vitro Study”","authors":"","doi":"10.1002/cre2.70125","DOIUrl":"https://doi.org/10.1002/cre2.70125","url":null,"abstract":"<p>David, S., Hüfner, H., Rauch, N., Kerberger, R., Drescher, D., Brunello, G., and Becker B. “Impact of Autoclaving on the Dimensional Stability of 3D-Printed Guides for Orthodontic Mini-Implant Insertion—An In Vitro Study.” <i>Clinical and Experimental Dental Research</i> 11, 2025: e70111.</p><p>On page 1, the text “Samuel David and Mira Hüfner contributed equally to this article.” is incorrect and should not have appeared. Samuel David is the first author and Mira Hüfner is the second author, with no shared first authorship.</p><p>We apologize for this error.</p>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70125","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143801385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gray H. Li, Vidya Mudaliar, Andrew B. Cameron, John M. Aarts, Joanne J. E. Choi
Objectives
To evaluate the effect of the size of stainless steel mesh and universal adhesive primer on the flexural strength of repaired polymethylmethacrylate (PMMA) denture base resin.
Materials and Methods
A total of 120 heat-cured PMMA specimens with dimensions of 5 × 50 × 30 mm were prepared and repaired with two different sizes of stainless steel mesh reinforcement, one group with med-fine mesh (size 0.42 mm2) and the second group with fine mesh (size 0.09 mm2). One subgroup was primed with a universal adhesive primer (Scotchbond Universal). Half of the specimens from each subgroup were subjected to artificial aging. The flexural strength was obtained by three-point bend testing. Data were statistically analyzed using ANOVA and post hoc analysis (SPSS V28). The probability of failure was calculated using Weibull analysis. Scanning electron microscopy analysis was used to identify the mode of failure.
Results
A significantly higher mean flexural strength (p < 0.05) was recorded in primed groups non-thermocycled with fine mesh (174.80 ± 50.27 MPa) and medium mesh (160.87 ± 41.50 MPa) compared to non-primed groups. Non-primed specimens with fine mesh exhibited the highest Weibull modulus (5.86), whereas that of primed medium mesh had the lowest Weibull modulus (2.64). Adhesive failure was identified at the interface of the stainless steel mesh and the self-cure acrylic resin.
Conclusion
Application of the universal adhesive primer to both mid-fine and fine stainless steel mesh significantly improved the flexural strength of the repaired PMMA heat-cured acrylic resin, and reinforcement with primed fine stainless steel mesh resulted in significantly higher flexural strength of repaired PMMA heat-cured an acrylic resin.
{"title":"Influence of Stainless Steel Mesh Size and Universal Adhesive Primer on Flexural Strength of Repaired PMMA Denture Base Resin","authors":"Gray H. Li, Vidya Mudaliar, Andrew B. Cameron, John M. Aarts, Joanne J. E. Choi","doi":"10.1002/cre2.70127","DOIUrl":"https://doi.org/10.1002/cre2.70127","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>To evaluate the effect of the size of stainless steel mesh and universal adhesive primer on the flexural strength of repaired polymethylmethacrylate (PMMA) denture base resin.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>A total of 120 heat-cured PMMA specimens with dimensions of 5 × 50 × 30 mm were prepared and repaired with two different sizes of stainless steel mesh reinforcement, one group with med-fine mesh (size 0.42 mm<sup>2</sup>) and the second group with fine mesh (size 0.09 mm<sup>2</sup>). One subgroup was primed with a universal adhesive primer (Scotchbond Universal). Half of the specimens from each subgroup were subjected to artificial aging. The flexural strength was obtained by three-point bend testing. Data were statistically analyzed using ANOVA and post hoc analysis (SPSS V28). The probability of failure was calculated using Weibull analysis. Scanning electron microscopy analysis was used to identify the mode of failure.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A significantly higher mean flexural strength (<i>p</i> < 0.05) was recorded in primed groups non-thermocycled with fine mesh (174.80 ± 50.27 MPa) and medium mesh (160.87 ± 41.50 MPa) compared to non-primed groups. Non-primed specimens with fine mesh exhibited the highest Weibull modulus (5.86), whereas that of primed medium mesh had the lowest Weibull modulus (2.64). Adhesive failure was identified at the interface of the stainless steel mesh and the self-cure acrylic resin.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Application of the universal adhesive primer to both mid-fine and fine stainless steel mesh significantly improved the flexural strength of the repaired PMMA heat-cured acrylic resin, and reinforcement with primed fine stainless steel mesh resulted in significantly higher flexural strength of repaired PMMA heat-cured an acrylic resin.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70127","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143786637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Survivin is used to determine the prognosis and clinical features of premalignant and malignant lesions. The aim of this study was to determine the correlation between the expression of survivin isoforms and clinical outcomes in oral lichen planus and oral squamous cell carcinoma.
Materials and Methods
This cross-sectional study examined 119 cases, including oral squamous cell carcinoma (SCC), oral lichen planus (OLP), and healthy margins of lesions. For all lesions, survivin expression was assessed quantitatively and qualitatively using real-time polymerase chain reaction. The data were analyzed using SPSS 20.
Results
The expression of survivin-∆EX3 and survivin-2B were quantitatively and qualitatively higher in SCC and OLP cases than in healthy mucosa (p < 0.05). The mean expression of survivin-∆EX3 in erosive OLP (4.95 ± 4.41) was higher than that in nonerosive OLP (2.13 ± 3.32, p < 0.05). Moreover, the mean expression of both genes was significantly higher in different grades of SCC compared to healthy mucosa (p < 0.05). There was also a significant correlation between gene expressions (p < 0.001).
Conclusion
The increased expression of survivin-∆EX3 and survivin-2B in OSCC correlates with tumor progression and advanced clinical stages, suggesting a potential prognostic role.
{"title":"Comparative Study of the Expressions of Nuclear (∆EX3) and Cytoplasmic (2B) Survivins in Oral Squamous Cell Carcinoma and Oral Lichen Planus Using Real-Time PCR","authors":"Maryam Amirchaghmaghi, Atessa Pakfetrat, Nooshin Mohtasham, Farnaz Mohajertehran, Mohammad Taghi shakeri, Elahe Vazavandi","doi":"10.1002/cre2.70123","DOIUrl":"https://doi.org/10.1002/cre2.70123","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>Survivin is used to determine the prognosis and clinical features of premalignant and malignant lesions. The aim of this study was to determine the correlation between the expression of survivin isoforms and clinical outcomes in oral lichen planus and oral squamous cell carcinoma.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>This cross-sectional study examined 119 cases, including oral squamous cell carcinoma (SCC), oral lichen planus (OLP), and healthy margins of lesions. For all lesions, survivin expression was assessed quantitatively and qualitatively using real-time polymerase chain reaction. The data were analyzed using SPSS 20.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The expression of survivin-∆EX3 and survivin-2B were quantitatively and qualitatively higher in SCC and OLP cases than in healthy mucosa (<i>p</i> < 0.05). The mean expression of survivin-∆EX3 in erosive OLP (4.95 ± 4.41) was higher than that in nonerosive OLP (2.13 ± 3.32, <i>p</i> < 0.05). Moreover, the mean expression of both genes was significantly higher in different grades of SCC compared to healthy mucosa (<i>p</i> < 0.05). There was also a significant correlation between gene expressions (<i>p</i> < 0.001).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The increased expression of survivin-∆EX3 and survivin-2B in OSCC correlates with tumor progression and advanced clinical stages, suggesting a potential prognostic role.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70123","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143749355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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