Ying-li Liu, Xuan-wei Chen, Si-qi Tian, Xiao-hua Tan and Bo Peng*,
{"title":"土霉素耐药后,巴尔达爱德华氏菌(Edwardsiella tarda)的毒性减弱。","authors":"Ying-li Liu, Xuan-wei Chen, Si-qi Tian, Xiao-hua Tan and Bo Peng*, ","doi":"10.1021/acs.jproteome.4c00303","DOIUrl":null,"url":null,"abstract":"<p >The relationship between antibiotic resistance and bacterial virulence has not yet been fully explored. Here, we use <i>Edwardsiella tarda</i> as the research model to investigate the proteomic change upon oxytetracycline resistance (LTB4-R<sub>OTC</sub>). Compared to oxytetracycline-sensitive <i>E. tarda</i> (LTB4-S), LTB4-R<sub>OTC</sub> has 234 differentially expressed proteins, of which the abundance of 84 proteins is downregulated and 15 proteins are enriched to the Type III secretion system, Type VI secretion system, and flagellum pathways. Functional analysis confirms virulent phenotypes, including autoaggregation, biofilm formation, hemolysis, swimming, and swarming, are impaired in LTB4-R<sub>OTC</sub>. Furthermore, the <i>in vivo</i> bacterial challenge in both tilapia and zebrafish infection models suggests that the virulence of LTB4-R<sub>OTC</sub> is attenuated. Analysis of immune gene expression shows that LTB4-R<sub>OTC</sub> induces a stronger immune response in the spleen but a weaker response in the head kidney than that induced by LTB4-S, suggesting it's a potential vaccine candidate. Zebrafish and tilapia were challenged with a sublethal dose of LTB4-R<sub>OTC</sub> as a live vaccine followed by LTB4-S challenge. The relative percentage of survival of zebrafish is 60% and that of tilapia is 75% after vaccination. Thus, our study suggests that bacteria that acquire antibiotic resistance may attenuate virulence, which can be explored as a potential live vaccine to tackle bacterial infection in aquaculture.</p>","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":null,"pages":null},"PeriodicalIF":3.8000,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Edwardsiella tarda Attenuates Virulence upon Oxytetracycline Resistance\",\"authors\":\"Ying-li Liu, Xuan-wei Chen, Si-qi Tian, Xiao-hua Tan and Bo Peng*, \",\"doi\":\"10.1021/acs.jproteome.4c00303\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >The relationship between antibiotic resistance and bacterial virulence has not yet been fully explored. Here, we use <i>Edwardsiella tarda</i> as the research model to investigate the proteomic change upon oxytetracycline resistance (LTB4-R<sub>OTC</sub>). Compared to oxytetracycline-sensitive <i>E. tarda</i> (LTB4-S), LTB4-R<sub>OTC</sub> has 234 differentially expressed proteins, of which the abundance of 84 proteins is downregulated and 15 proteins are enriched to the Type III secretion system, Type VI secretion system, and flagellum pathways. Functional analysis confirms virulent phenotypes, including autoaggregation, biofilm formation, hemolysis, swimming, and swarming, are impaired in LTB4-R<sub>OTC</sub>. Furthermore, the <i>in vivo</i> bacterial challenge in both tilapia and zebrafish infection models suggests that the virulence of LTB4-R<sub>OTC</sub> is attenuated. Analysis of immune gene expression shows that LTB4-R<sub>OTC</sub> induces a stronger immune response in the spleen but a weaker response in the head kidney than that induced by LTB4-S, suggesting it's a potential vaccine candidate. Zebrafish and tilapia were challenged with a sublethal dose of LTB4-R<sub>OTC</sub> as a live vaccine followed by LTB4-S challenge. The relative percentage of survival of zebrafish is 60% and that of tilapia is 75% after vaccination. Thus, our study suggests that bacteria that acquire antibiotic resistance may attenuate virulence, which can be explored as a potential live vaccine to tackle bacterial infection in aquaculture.</p>\",\"PeriodicalId\":48,\"journal\":{\"name\":\"Journal of Proteome Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2024-06-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Proteome Research\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://pubs.acs.org/doi/10.1021/acs.jproteome.4c00303\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Proteome Research","FirstCategoryId":"99","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acs.jproteome.4c00303","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Edwardsiella tarda Attenuates Virulence upon Oxytetracycline Resistance
The relationship between antibiotic resistance and bacterial virulence has not yet been fully explored. Here, we use Edwardsiella tarda as the research model to investigate the proteomic change upon oxytetracycline resistance (LTB4-ROTC). Compared to oxytetracycline-sensitive E. tarda (LTB4-S), LTB4-ROTC has 234 differentially expressed proteins, of which the abundance of 84 proteins is downregulated and 15 proteins are enriched to the Type III secretion system, Type VI secretion system, and flagellum pathways. Functional analysis confirms virulent phenotypes, including autoaggregation, biofilm formation, hemolysis, swimming, and swarming, are impaired in LTB4-ROTC. Furthermore, the in vivo bacterial challenge in both tilapia and zebrafish infection models suggests that the virulence of LTB4-ROTC is attenuated. Analysis of immune gene expression shows that LTB4-ROTC induces a stronger immune response in the spleen but a weaker response in the head kidney than that induced by LTB4-S, suggesting it's a potential vaccine candidate. Zebrafish and tilapia were challenged with a sublethal dose of LTB4-ROTC as a live vaccine followed by LTB4-S challenge. The relative percentage of survival of zebrafish is 60% and that of tilapia is 75% after vaccination. Thus, our study suggests that bacteria that acquire antibiotic resistance may attenuate virulence, which can be explored as a potential live vaccine to tackle bacterial infection in aquaculture.
期刊介绍:
Journal of Proteome Research publishes content encompassing all aspects of global protein analysis and function, including the dynamic aspects of genomics, spatio-temporal proteomics, metabonomics and metabolomics, clinical and agricultural proteomics, as well as advances in methodology including bioinformatics. The theme and emphasis is on a multidisciplinary approach to the life sciences through the synergy between the different types of "omics".