黄嘌呤对斑马鱼胚胎的发育毒性试验。

IF 3.9 3区 环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Comparative Biochemistry and Physiology C-toxicology & Pharmacology Pub Date : 2024-06-08 DOI:10.1016/j.cbpc.2024.109957
Liyan Xu , Yuxin Shi , Jing Huang , Lixin Feng , Yuxin Wang , Attila Gabor SIK , Xiqiang Chen , Kechun Liu , Rongchun Wang , Meng Jin
{"title":"黄嘌呤对斑马鱼胚胎的发育毒性试验。","authors":"Liyan Xu ,&nbsp;Yuxin Shi ,&nbsp;Jing Huang ,&nbsp;Lixin Feng ,&nbsp;Yuxin Wang ,&nbsp;Attila Gabor SIK ,&nbsp;Xiqiang Chen ,&nbsp;Kechun Liu ,&nbsp;Rongchun Wang ,&nbsp;Meng Jin","doi":"10.1016/j.cbpc.2024.109957","DOIUrl":null,"url":null,"abstract":"<div><p>Xanthatin (XAN), a xanthanolide sesquiterpene lactone, isolated from Chinese herb, <em>Xanthium strumarium L</em>, has various pharmacological activities, such as antitumor activity and anti-inflammatory. However, little is known about its potential toxicity and the mechanism. Here, zebrafish model was used to study the developmental toxicity <em>in vivo</em>. Our results indicated that xanthatin increased the mortality and led to the morphological abnormalities including pericardial edema, yolk sac edema, curved body shape and hatching delay. Furthermore, xanthatin damaged the normal structure and/or function of heart, liver, immune and nervous system. ROS elevation and much more apoptosis cells were observed after xanthatin exposure. Gene expression results showed that oxidative stress-related genes <em>nrf2</em> was inhibited, while oxidative stress-related genes (<em>keap1</em> and <em>nqo1</em>) and apoptotic genes (<em>caspase3</em>, <em>caspase9</em> and <em>p53</em>) were increased after xanthatin exposure. Mitophagy related genes <em>pink1</em> and <em>parkin</em>, and wnt pathway (<em>β-catenin</em>, <em>wnt8a</em> and <em>wnt11</em>) were significantly increased after xanthatin exposure. Taken together, our finding indicated that xanthatin induced developmental toxicity, and the ROS elevation, apoptosis activation, dysregulation of mitophagy and wnt pathways were involved in the toxicity caused by xanthatin.</p></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":null,"pages":null},"PeriodicalIF":3.9000,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Developmental toxicity assay of xanthatin in zebrafish embryos\",\"authors\":\"Liyan Xu ,&nbsp;Yuxin Shi ,&nbsp;Jing Huang ,&nbsp;Lixin Feng ,&nbsp;Yuxin Wang ,&nbsp;Attila Gabor SIK ,&nbsp;Xiqiang Chen ,&nbsp;Kechun Liu ,&nbsp;Rongchun Wang ,&nbsp;Meng Jin\",\"doi\":\"10.1016/j.cbpc.2024.109957\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Xanthatin (XAN), a xanthanolide sesquiterpene lactone, isolated from Chinese herb, <em>Xanthium strumarium L</em>, has various pharmacological activities, such as antitumor activity and anti-inflammatory. However, little is known about its potential toxicity and the mechanism. Here, zebrafish model was used to study the developmental toxicity <em>in vivo</em>. Our results indicated that xanthatin increased the mortality and led to the morphological abnormalities including pericardial edema, yolk sac edema, curved body shape and hatching delay. Furthermore, xanthatin damaged the normal structure and/or function of heart, liver, immune and nervous system. ROS elevation and much more apoptosis cells were observed after xanthatin exposure. Gene expression results showed that oxidative stress-related genes <em>nrf2</em> was inhibited, while oxidative stress-related genes (<em>keap1</em> and <em>nqo1</em>) and apoptotic genes (<em>caspase3</em>, <em>caspase9</em> and <em>p53</em>) were increased after xanthatin exposure. Mitophagy related genes <em>pink1</em> and <em>parkin</em>, and wnt pathway (<em>β-catenin</em>, <em>wnt8a</em> and <em>wnt11</em>) were significantly increased after xanthatin exposure. Taken together, our finding indicated that xanthatin induced developmental toxicity, and the ROS elevation, apoptosis activation, dysregulation of mitophagy and wnt pathways were involved in the toxicity caused by xanthatin.</p></div>\",\"PeriodicalId\":10602,\"journal\":{\"name\":\"Comparative Biochemistry and Physiology C-toxicology & Pharmacology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2024-06-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Comparative Biochemistry and Physiology C-toxicology & Pharmacology\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S153204562400125X\",\"RegionNum\":3,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S153204562400125X","RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

Xanthatin(XAN)是从中草药Xanthium strumarium L中分离出来的一种黄烷内酯倍半萜内酯,具有多种药理活性,如抗肿瘤活性和抗炎作用。然而,人们对其潜在的毒性和机制知之甚少。在这里,我们利用斑马鱼模型来研究其体内发育毒性。研究结果表明,黄铂会增加斑马鱼的死亡率,并导致其形态异常,包括心包水肿、卵黄囊水肿、体形弯曲和孵化延迟。此外,黄嘌呤还损害了心脏、肝脏、免疫和神经系统的正常结构和/或功能。暴露于黄嘌呤后,观察到 ROS 升高和更多的细胞凋亡。基因表达结果显示,与氧化应激相关的基因nrf2受到抑制,而与氧化应激相关的基因(keap1和nqo1)和凋亡基因(caspase3、caspase9和p53)在接触黄嘌呤后增加。与丝裂噬相关的基因pink1和parkin以及wnt通路(β-catenin、wnt8a和wnt11)在接触黄嘌呤后显著增加。总之,我们的研究结果表明,黄嘌呤诱导发育毒性,ROS升高、细胞凋亡激活、有丝分裂和wnt通路失调参与了黄嘌呤的毒性作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Developmental toxicity assay of xanthatin in zebrafish embryos

Xanthatin (XAN), a xanthanolide sesquiterpene lactone, isolated from Chinese herb, Xanthium strumarium L, has various pharmacological activities, such as antitumor activity and anti-inflammatory. However, little is known about its potential toxicity and the mechanism. Here, zebrafish model was used to study the developmental toxicity in vivo. Our results indicated that xanthatin increased the mortality and led to the morphological abnormalities including pericardial edema, yolk sac edema, curved body shape and hatching delay. Furthermore, xanthatin damaged the normal structure and/or function of heart, liver, immune and nervous system. ROS elevation and much more apoptosis cells were observed after xanthatin exposure. Gene expression results showed that oxidative stress-related genes nrf2 was inhibited, while oxidative stress-related genes (keap1 and nqo1) and apoptotic genes (caspase3, caspase9 and p53) were increased after xanthatin exposure. Mitophagy related genes pink1 and parkin, and wnt pathway (β-catenin, wnt8a and wnt11) were significantly increased after xanthatin exposure. Taken together, our finding indicated that xanthatin induced developmental toxicity, and the ROS elevation, apoptosis activation, dysregulation of mitophagy and wnt pathways were involved in the toxicity caused by xanthatin.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
7.50
自引率
5.10%
发文量
206
审稿时长
30 days
期刊介绍: Part C: Toxicology and Pharmacology. This journal is concerned with chemical and drug action at different levels of organization, biotransformation of xenobiotics, mechanisms of toxicity, including reactive oxygen species and carcinogenesis, endocrine disruptors, natural products chemistry, and signal transduction with a molecular approach to these fields.
期刊最新文献
Assessing antioxidant responses in C6 and U-87 MG cell lines exposed to high copper levels. Deficiency of PvDRAM2 increased the nitrite sensitivity of Pacific white shrimp (Penaeus vannamei) by inhibiting autophagy. Effect of lead on photosynthetic pigments, antioxidant responses, metabolomics, thalli morphology and cell ultrastructure of Iridaea cordata (Rhodophyta) from Antarctica Tire rubber-derived contaminant 6PPD had the potential to induce metabolism disorder in early developmental stage of zebrafish Effects of water immersion on immune, intestinal flora and metabolome of Chinese mitten crab (Eriocheir sinensis) after air exposure
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1